Intake and excretion of arsenicals in green (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata)
Tetsuro Agusa A B , Kozue Takagi B , Todd W. Miller B , Reiji Kubota C , Yasumi Anan D , Hisato Iwata B and Shinsuke Tanabe B E
+ Author Affiliations
- Author Affiliations
A Department of Legal Medicine, Shimane University Faculty of Medicine, Enya 89-1, Izumo, 693-8501, Japan.
B Center for Marine Environmental Studies (CMES), Ehime University, Bunkyo-cho 2-5, Matsuyama, 790-8577, Japan.
C Division of Environmental Chemistry, National Institute of Health Sciences, Kamiyoga 1-18-1, Setagaya-ku, Tokyo, 158-8501, Japan.
D Laboratory of Chemical Toxicology and Environmental Health, Showa Pharmaceutical University, 3-3165 Higashi-Tamagawagakuen, Machida, Tokyo, 194-8543, Japan.
E Corresponding author. Email: shinsuke@agr.ehime-u.ac.jp
We dedicate this paper to the memory of the late Professor Kaise in recognition of his lifelong work on environmental arsenic chemistry.
Environmental Chemistry 8(1) 19-29 https://doi.org/10.1071/EN10091
Submitted: 15 August 2010 Accepted: 27 October 2010 Published: 28 February 2011
Environmental context. Although among higher marine animals, relatively high concentration of arsenic and unique distribution of arsenic compounds are found in green (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata), the accumulation mechanism remains unknown. We examined the accumulation of arsenicals in two turtles from the standpoint of short- and long-term intake and excretion and found that prey items might be important for the arsenic accumulation. This study can provide useful information on the accumulation pattern of arsenic speciation in sea turtles.
Abstract. We analysed arsenic (As) compounds in the stomach and intestine contents, bile and urine of green (Chelonia mydas) and hawksbill turtles (Eretmochelys imbricata) to understand As accumulation through intake and excretion. Stable isotopes of δ15N and δ13C were also measured for understanding the feeding behaviour dependent accumulation of As. Major As species in gastrointestinal contents were unknown water-soluble As, followed by unextracted As. Concentrations of AB and DMA in the tissues were higher than those in the stomach contents (prey items), indicating high bioaccumulation of these arsenicals. In green turtles, AB concentration was high in bile and increased throughout the gastrointestinal tract, suggesting significant biliary excretion of AB. δ15N was positively correlated with AB level in green turtles, whereas a negative relationship between residual As and δ15N was observed in hawksbill turtles. This study indicates feeding behaviour-dependent accumulation of As compounds in both turtle species for the first time.
Additional keywords: arsenic compounds, bile, feeding habit, gastrointestinal contents, stable isotope ratios, urine.
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