First report of Tuberose mild mosaic potyvirus from tuberose (Polianthes tuberosa L.) in India
S. K. Raj A C , S. K. Snehi A , S. Kumar A , T. Ram B and A. K. Goel BA Plant Molecular Virology, National Botanical Research Institute, Lucknow 226 001, U.P., India.
B Botanic Gardens, National Botanical Research Institute, Lucknow 226 001, U.P., India.
C Corresponding author. Email: skraj2@rediffmail.com
Australasian Plant Disease Notes 4(1) 93-95 https://doi.org/10.1071/DN09040
Submitted: 18 February 2009 Accepted: 28 August 2009 Published: 9 September 2009
Abstract
A potyvirus was detected molecularly by reverse-transcription polymerase chain reaction (RT-PCR) and serologically by Ouchterlony’s double diffusion test from a tuberose (Polianthes tuberosa L.) plant with mild mosaic disease. Potyvirus group-degenerate primers specific to the core region of coat protein gene were used in RT-PCR and the subsequent amplicon was sequenced. The sequence shared highest 98% and 99% identities at nucleotide and amino acid level, respectively and had a close phylogenetic relationship with the sequence of Tuberose mild mosaic potyvirus (TuMMV). This is the first report of TuMMV being present in India.
Acknowledgements
The authors would like to thank the Director, N. B. R. I., Lucknow, for facilities and Council of Scientific and Industrial Research, New Delhi, for fellowship to S. Kumar.
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