Development of low-phytate maize inbred lines through marker-assisted introgression of lpa1
K. R. Yathish
A B , Chikkappa G. Karjagi B * , Shivraj S. Gangoliya B C , Raveendra N. Gadag A , M. G. Mallikarjuna A , Javaji C. Sekhar B , Abhijit K. Das B , P. Lakshmi Soujanya B , Ramesh Kumar B , Alla Singh B , Shyam Bir Singh B and Sujay Rakshit B
+ Author Affiliations
- Author Affiliations
A ICAR-Indian Agricultural Research Institute, Pusa Campus New Delhi, Delhi 110012, India.
B ICAR-Indian Institute of Maize Research, PAU Campus, Ludhiana, Punjab 141004, India.
C Maharana Pratap Government Post Graduate College, Gadarwara, Madhya Pradesh 487551, India.
Crop & Pasture Science 74(9) 843-855 https://doi.org/10.1071/CP22238
Submitted: 11 July 2022 Accepted: 4 February 2023 Published: 14 March 2023
© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Context: Phytic acid is the major storage form of phosphorus in cereals and is considered an anti-nutritional factor because it chelates major mineral micronutrient cations, resulting in micronutrient malnutrition in humans. For monogastric animals fed maize (Zea mays L.) grains, the stored phosphorus does not release into the digestive tract, leading to phosphorus deficiency and environmental pollution.
Aims: The aim of the study was to develop maize lines with a lower level of phytic acid that might substantially enhance the nutritional value of maize.
Methods: The lpa1 mutant allele conferring low phytic acid was transferred into the parental lines of popular maize hybrid DMH 121 (i.e. BML 6 and BML 45) through marker-assisted backcross breeding. Foreground selection was performed using a co-dominant single nucleotide polymorphism marker through a high-resolution melting approach, and background selection was undertaken using 50–55 polymorphic sequence-tagged microsatellite site markers.
Key results: Near-isogeneic lines were produced with >90% recurrent parental genome and reduction of phytic acid content by up to 44–56% compared with the original lines.
Conclusions: The near-isogeneic lines carrying lpa1 can be used to reconstitute DHM 121 with low phytate content.
Implications: The low-phytate maize hybrids produced can be useful in reducing micronutrient malnutrition in humans, as well as environmental pollution.
Keywords: biofortification, high-resolution melting, inorganic phosphorus, maize, malnutrition, marker-assisted backcross breeding, near isogenic lines, phytic acid.
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