RNA-Seq analysis of resistant and susceptible soybean genotypes in response to stress from soybean cyst nematode (Heterodera glycines) HG type 1.2.3.5.7
Haipeng Jiang A , Fanshan Bu A , Shixin Yan A , Yi Li A , Tong Wu A , RuiYao Bai A , Aitong Xu A , Xue Zhao
A and Yingpeng Han A *
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Soybean Biology in Chinese Ministry of Education (Northeastern Key Laboratory of Soybean Biology and Genetics and Breeding in Chinese Ministry of Agriculture), Northeast Agricultural University, Harbin 150030, China.
Crop & Pasture Science 73(3) 238-248 https://doi.org/10.1071/CP21447
Submitted: 25 June 2021 Accepted: 7 September 2021 Published: 22 December 2021
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
The soybean cyst nematode (SCN, Heterodera glycine Ichinohe) is a major disease affecting soybean (Glycine max (L.) Merr.) production and yield. Breeding of new SCN-resistant cultivars and understanding their resistance mechanisms are valuable for improving SCN resistance of soybean. However, we still know little about resistance mechanisms to SCN. The purpose of our analysis was to understand the different resistance mechanisms of resistant and susceptible lines to SCN by dissecting their transcriptional changes during infection by SCN HG type 1.2.3.5.7. In this study, 119 recombinant inbred lines derived from a cross of cv. Dongnong L-204 (SCN resistant) and cv. Heinong 37 (SCN susceptible) were identified for resistance status to SCN HG type 1.2.3.5.7. Roots of the lines identified as extremely resistant or susceptible (L5, L89) were sequenced by transcriptome, and we obtained 66.61 Gb of data. Compared with the untreated control, there were 8394 and 6899 differentially expressed genes in SCN-infected roots of L5 and L89, respectively. Genes of metabolic pathways, biosynthesis of secondary metabolites, plant hormone signal transduction, and plant–pathogen interaction pathway were significantly expressed in both resistant and susceptible genotypes. The expression of genes of phenylpropanoid biosynthesis, flavonoid biosynthesis, thiamine metabolism, cutin, suberin and wax biosynthesis, and endocytosis pathway was significantly higher in the resistant line than the susceptible line. Transcription factor analysis showed that 88 transcription factors from 18 transcription factor families responded to SCN stress. Nine genes were identified by reverse transcriptase qPCR to be associated with SCN resistance. This study helps us to understand better the mechanism of soybean resistance to SCN.
Keywords: DEGs, HG type 1.2.3.5.7 (race 4), metabolic pathways, qPCR, resistance mechanisms, soybean cyst nematode, transcription factor, transcriptome.
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