Synthesis of heat-shock proteins HSP-70 and HSP-90 in flowers of common buckwheat (Fagopyrum esculentum) under thermal stress
A. Płażek
A , K. Hura A , T. Hura B D , A. Słomka C , M. Hornyák A and K. Sychta C
+ Author Affiliations
- Author Affiliations
A Department of Plant Breeding, Physiology and Seed Science, University of Agriculture, Podłużna 3, 30-239 Kraków, Poland.
B Polish Academy of Sciences, The Franciszek Górski Institute of Plant Physiology, Niezapominajek 21, 30-239 Kraków, Poland.
C Institute of Botany, Department of Plant Cytology and Embryology, Jagiellonian University, Gronostajowa 9, 30-387 Kraków, Poland.
D Corresponding author. Email: t.hura@ifr-pan.edu.pl
Crop and Pasture Science 71(8) 760-767 https://doi.org/10.1071/CP20011
Submitted: 14 January 2020 Accepted: 19 June 2020 Published: 16 July 2020
Abstract
Common buckwheat (Fagopyrum esculentum Moench) is a valuable crop plant with cereal-like seed chemical composition; however, it is susceptible to thermal stress. The aim of the study was to determine whether heat-shock proteins HSP-90 and HSP-70 can protect common buckwheat against thermal stress during development of microspores and embryo sacs. The study was performed on two accessions of common buckwheat, Panda and PA15, which differed in their tolerance to thermal stress. Accumulation of these proteins was determined in buds, open and wilted flowers, and donor leaves of plants grown at 20°C (control) and 30°C (thermal stress). Photochemical efficiency of donor leaves, closest to the inflorescences, based on chlorophyll a fluorescence (ChlF) was also analysed. All plants demonstrated higher values of ChlF at 30°C than at 20°C, which suggests that this 30°C temperature is more conducive to their vegetative growth. Pollen grains of both accessions demonstrated normal development at 30°C, whereas embryo sacs showed many developmental disturbances. Panda was more sensitive to thermal stress than PA15, as manifested in a higher percentage of degenerated embryo sacs at the flower bud phase. Moreover, a decrease in both HSPs in the studied organs of Panda was found relative to the control. At 30°C, both accessions accumulated more HSP-70 than HSP-90. These results suggest that, under heat stress, HSP-70 plays a protective role for flowers of common buckwheat. The analyses indicated that the donor leaf closest to the flower cluster may be a reliable indicator of temperature sensitivity in buckwheat flowers.
Additional keywords: chlorophyll fluorescence, embryological analyses, stress tolerance, western-blot.
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