Identification of new resistance sources to powdery mildew, and the genetic characterisation of resistance in three common bean genotypes
Ester Murube A , Ana Campa A and Juan José Ferreira A B
+ Author Affiliations
- Author Affiliations
A Plant Genetic Group, Horticultural and Forest Crops, SERIDA, Asturias, Spain.
B Corresponding author. Email: jjferreira@serida.org
Crop and Pasture Science 68(11) 1006-1012 https://doi.org/10.1071/CP16460
Submitted: 16 December 2016 Accepted: 4 April 2017 Published: 5 June 2017
Abstract
Powdery mildew (PM) is a devastating disease of many legume species, including common bean. In this work, we assessed the responses of 108 dry and snap bean accessions to PM, and characterised the genetic control of the resistance in three bean genotypes. Resistance tests under controlled conditions led to the identification of 11 dry bean accessions with total resistance. However, no snap bean accessions showed total resistance, although two cultivars showed mixed phenotypes including seedlings with total resistance. The inheritance of resistance was analysed in three F2:3 populations involving the resistant bean genotypes BelNeb, G19833 and BGE003161. In the three populations, the segregation for PM resistance fit the expected ratio for one dominant gene. The resistance loci were mapped to the beginning of the linkage group Pv04. The physical positions of the flanking markers indicated that the three resistance genes were located between the physical positions 0 and 1.09 Mb. This work provides new PM-resistance sources and markers linked to resistance genes, which will be very useful in common bean breeding programs focussed on protecting bean crops against this disease.
Additional keywords: genetic linkage map, marker-assisted selection, Phaseolus vulgaris L., plant breeding, resistance sources.
References
Almeida AMR, Binneck E, Piuga FF, Marin SRR, do Ribeiro Valle PRZ, Silveira CZ (2008) Characterization of powdery mildews strains from soybean, bean, sunflower, and weeds in Brazil using rDNA-ITS sequences. Tropical Plant Pathology 33, 20–26.| Characterization of powdery mildews strains from soybean, bean, sunflower, and weeds in Brazil using rDNA-ITS sequences.Crossref | GoogleScholarGoogle Scholar |
Bai Y, Pavan S, Zheng Z, Zappel NF, Reinstädler A, Lotti C, De Giovanni C, Ricciardi L, Lindhout P, Visser R, Theres K, Panstruga R (2008) Naturally occurring broad-spectrum powdery mildew resistance in a central American tomato accession is caused by loss of Mlo function. Molecular Plant-Microbe Interactions 21, 30–39.
| Naturally occurring broad-spectrum powdery mildew resistance in a central American tomato accession is caused by loss of Mlo function.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXhsVOrtr3J&md5=b73590030b7fbefddfdb7277b043e94fCAS |
Bett KE, Michaels TE (1995) A two-gene model for powdery mildew resistance in common bean. Annual Report of the Bean Improvement Cooperative 38, 145–146.
Blair MW, Pedraza F, Buendia HF, Gaitán-Solis E, Beebe S, Gepts P, Tohme J (2003) Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 107, 1362–1374.
| Development of a genome-wide anchored microsatellite map for common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXovVOhu7g%3D&md5=c11180dda89aa67b132faca31c0779f5CAS |
Büschges R, Hollricher K, Panstruga R, Simons G, Wolter M, Frijters A, van Daelen R, van der Lee T, Diergaarde P, Groenendijk J, Töpsch S, Vos P, Salamini F, Schulze-Lefert P (1997) The barley Mlo gene: a novel control element of plant pathogen resistance. Cell 88, 695–705.
| The barley Mlo gene: a novel control element of plant pathogen resistance.Crossref | GoogleScholarGoogle Scholar |
Campa A, Ferreira JJ (2017) Gene codifying an elongation factor is involved in the resistance against powdery mildew in common bean. Theoretical and Applied Genetics 130, 849–860.
| Gene codifying an elongation factor is involved in the resistance against powdery mildew in common bean.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2sXjsFGjsLg%3D&md5=cbd5b607d6ab57670c71d625b8e058f7CAS |
Consonni C, Humphry ME, Hartmann HA, Livaja M, Durner J, Westphal L, Vogel J, Lipka V, Kemmerling B, Schulze-Lefert P, Somerville SC, Panstruga R (2006) Conserved requirements for a plant host cell protein in powdery mildew pathogenesis. Nature Genetics 38, 716–720.
| Conserved requirements for a plant host cell protein in powdery mildew pathogenesis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XltVOhurs%3D&md5=8677bc5bf9ee89ac360a72e7a72d0360CAS |
Dundas B (1936) Inheritance of resistance to powdery mildew in beans. Hilgardia 10, 243–253.
| Inheritance of resistance to powdery mildew in beans.Crossref | GoogleScholarGoogle Scholar |
Ferreira RV, Ramalho MAP, Corte HR (1999) Genetic control of common bean (Phaseolus vulgaris L.) resistance to powdery mildew (Erysiphe polygoni). Genetics and Molecular Biology 22, 233–236.
| Genetic control of common bean (Phaseolus vulgaris L.) resistance to powdery mildew (Erysiphe polygoni).Crossref | GoogleScholarGoogle Scholar |
Ferreira RV, Bosco dos Santos J, Patto MA, Furtado D (2001) Agronomical characters and RAPD markers associated with the resistant allele to the Erysiphe polygoni in common bean. Crop Breeding and Applied Biotechnology 1, 11–21.
| Agronomical characters and RAPD markers associated with the resistant allele to the Erysiphe polygoni in common bean.Crossref | GoogleScholarGoogle Scholar |
Ferreira JJ, Murube E, Campa A (2017) Introgressed genomic regions in a set of near-isogenic lines of common bean revealed by genotyping-by-sequencing. The Plant Genome 10,
| Introgressed genomic regions in a set of near-isogenic lines of common bean revealed by genotyping-by-sequencing.Crossref | GoogleScholarGoogle Scholar |
Fondevilla S, Rubiales D (2012) Powdery mildew control in pea. A review. Agronomy for Sustainable Development 32, 401–409.
| Powdery mildew control in pea. A review.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XksVOgsbg%3D&md5=6521ea2ba3e4da671224545ba4a71a4fCAS |
Glawe DA (2008) The powdery mildews. A review of the world’s most familiar (yet poorly known) plant pathogens. Annual Review of Phytopathology 46, 27–51.
| The powdery mildews. A review of the world’s most familiar (yet poorly known) plant pathogens.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtFCqt77I&md5=263f3727690ad729a2d6ed2476529693CAS |
Hückelhoven R, Panstruga R (2011) Cell biology of the plant–powdery mildew interaction. Current Opinion in Plant Biology 14, 738–746.
| Cell biology of the plant–powdery mildew interaction.Crossref | GoogleScholarGoogle Scholar |
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.
| MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXhsVCksrk%3D&md5=43cd77505e3099d64a30eacf12d66855CAS |
Leitão ST, Almeida NF, Moral A, Rubiales D, Vaz Patto C (2013) Identification of resistance to rust (Uromyces appendiculatus) and powdery mildew (Erysiphe diffusa) in Portuguese common bean germplasm. Plant Breeding 132, 654–657.
| Identification of resistance to rust (Uromyces appendiculatus) and powdery mildew (Erysiphe diffusa) in Portuguese common bean germplasm.Crossref | GoogleScholarGoogle Scholar |
Mains EB, Dietz SM (1930) Physiologic forms of barley mildew, Erysiphe graminis Hordei Marchal. Phytopathology 20, 229–239.
Meziadi C, Richard MMS, Derquennes A, Thareau V, Blanchet S, Gratias A, Pflieger S, Geffroy V (2016) Development of molecular markers linked to disease resistance genes in common bean based on whole genome sequence. Plant Science 242, 351–357.
| Development of molecular markers linked to disease resistance genes in common bean based on whole genome sequence.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhsFaru7nF&md5=2d0c4d801ad9159593bfb0ee67c4ac78CAS |
Moghaddam SM, Song Q, Mamidi S, Schmutz J, Lee R, Cregan P, Osorno JM, McClean PE (2013) Developing market class specific InDel markers from next generation sequence data in Phaseolus vulgaris L. Frontiers in Plant Science 4, 251
| Developing market class specific InDel markers from next generation sequence data in Phaseolus vulgaris L.Crossref | GoogleScholarGoogle Scholar |
Nodari RO, Tsai SM, Gilbertson RL, Gepts P (1993) Towards an integrated linkage map of common bean 2. Development of an RFLP-based linkage map. Theoretical and Applied Genetics 85, 513–520.
| Towards an integrated linkage map of common bean 2. Development of an RFLP-based linkage map.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXitlSgu7g%3D&md5=32b555b8fdbfaa6e8b936f6ccfa5f063CAS |
Pavan S, Schiavulli A, Appiano M, Marcotrigiano A, Cillo F, Visser R, Bai Y, Lotti C, Ricciardi L (2011) Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus. Theoretical and Applied Genetics 123, 1425–1431.
| Pea powdery mildew er1 resistance is associated to loss-of-function mutations at a MLO homologous locus.Crossref | GoogleScholarGoogle Scholar |
Pérez-Vega E, Campa A, De la Rosa L, Giraldez R, Ferreira JJ (2009) Genetic diversity in a core collection established from the main bean genebank in Spain. Crop Science 49, 1377–1386.
| Genetic diversity in a core collection established from the main bean genebank in Spain.Crossref | GoogleScholarGoogle Scholar |
Pérez-Vega E, Trabanco N, Campa A, Ferreira JJ (2013) Genetic mapping of two genes conferring resistance to powdery mildew in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 126, 1503–1512.
| Genetic mapping of two genes conferring resistance to powdery mildew in common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Puerta Romero J (1962) ‘Enfermedades y plagas de judía.’ Hoja Divulgativa 11–12. pp. 28. (INIA: Madrid)
Rispail N, Rubiales D (2016) Genome-wide identification and comparison of legume MLO gene family. Scientific Reports 6, 32673
| Genome-wide identification and comparison of legume MLO gene family.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28XhsVymtr7F&md5=fff17bf9536022651c867129323539d2CAS |
Schmutz J, McClean PE, Mamidi S, Wu GA, Cannon SB, Grimwood J, Jenkins J, Shu S, Song Q, Chavarro C, Torres , Torres M, Geffroy V, Moghaddam SM, Gao D, Abernathy B, Barry K, Blair M, Brick MA, Chovatia M, Gepts P, Goodstein D, Gonzales M, Hellsten U, Hyten D, Jia G, Kelly JD, Kudrna D, Lee R, Richard M, Miklas PN, Osorno JM, Rodrigues J, Thareau V, Urrea CA, Wang M, Yu Y, Zhang M, Wing RA, Cregan PB, Rokhsar DS, Jackson SA (2014) A reference genome for common bean and genome-wide analysis of dual domestications. Nature Genetics 46, 707–713.
| A reference genome for common bean and genome-wide analysis of dual domestications.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXpsVamtr4%3D&md5=efd23e5d5464a0cf50f5d5a495ec248dCAS |
Schwartz HF, Katherman MJ, Thung MDT (1981) Yield response and resistance of dry beans to powdery mildew in Colombia. Plant Disease 65, 737–738.
| Yield response and resistance of dry beans to powdery mildew in Colombia.Crossref | GoogleScholarGoogle Scholar |
Schwartz HF, Steadman JR, Hall R, Forster RL (Eds) (2005) ‘Compendium of bean diseases.’ (The American Phytopathological Society: St Paul, MN, USA)
Stavely JR, Steadman JR, Coyne DP, Lindgre DT (1989) BelNeb rust resistance 1 and 2 great northern dry bean germplasm. HortSci 24, 400–401.
Trabanco N, Pérez-Vega E, Campa A, Rubiales D, Ferreira JJ (2012) Genetic resistance to powdery mildew in common bean. Euphytica 186, 875–882.
| Genetic resistance to powdery mildew in common bean.Crossref | GoogleScholarGoogle Scholar |
Vlasova A, Capella-Gutiérrez S, Rendón-Anaya M, Hernández-Oñate M, Minoche AE, Erb I, Câmara F, Prieto-Barja P, Corvelo A, Sanseverino W, Westergaard G, Dohm JC, Pappas GJ, Saburido-Alvarez S, Kedra D, Gonzalez I, Cozzuto L, Gómez-Garrido J, Aguilar-Morón MA, Andreu N, Aguilar M, Garcia-Mas J, Zehnsdorf M, Vázquez MP, Delgado-Salinas A, Delaye L, Lowy E, Mentaberry A, Vianello-Brondani RP, García JL, Alioto T, Sánchez F, Himmelbauer H, Santalla M, Notredame C, Gabaldón T, Herrera-Estrella A, Guigó R (2016) Genome and transcriptome analysis of the Mesoamerican common bean and the role of gene duplications in establishing tissue and temporal specialization of genes. Genome Biology 17, 32
| Genome and transcriptome analysis of the Mesoamerican common bean and the role of gene duplications in establishing tissue and temporal specialization of genes.Crossref | GoogleScholarGoogle Scholar |
Voysest O (2000) ‘Mejoramiento genético del frijol (Phaseolus vulgaris L.): Legado de variedades de América Latina 1930–1999.’ (Centro Internacional de Agricultura Tropical, CIAT: Cali, CO)
