Molecular diversity and nutriment studies of common bean (Phaseolus vulgaris L.) from the two hot-spots of Western Himalayas of Jammu and Kashmir
Neeraj Choudhary A , Anjali
B , Moni Gupta B , Safoora Shafi C , Sofora Jan C , Asma Hamid Mir C , Bikram Singh A and Reyazul Rouf Mir C *
+ Author Affiliations
- Author Affiliations
A Division of Plant Breeding and Genetics, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (SKUAST-J), Chatha, Jammu, Jammu & Kashmir, India.
B Division of Biochemistry, Sher-e-Kashmir University of Agricultural Sciences & Technology of Jammu (SKUAST-J), Chatha, Jammu, Jammu & Kashmir, India.
C Division of Genetics and Plant Breeding, Faculty of Agriculture (FoA), Sher-e-Kashmir University of Agricultural Sciences & Technology of Kashmir (SKUAST-K), Wadura Campus, Sopore, Jammu & Kashmir, India.
Crop & Pasture Science 73(3) 249-262 https://doi.org/10.1071/CP21347
Submitted: 19 May 2021 Accepted: 22 October 2021 Published: 12 January 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
The diverse microclimatic belts of the Western Himalayan region of India are considered hot spots for genetic diversity of common bean (Phaseolus vulgaris L.). Western Himalayan beans are known for various agronomically superior/important traits including unique aroma, taste and cooking quality. In the present study, 25 unlinked genomic simple sequence repeat (SSR) markers distributed across the common bean genome were used to assess the genetic/allelic diversity among and within populations belonging to the Jammu and Kashmir regions of the Western Himalayas. These two regions are considered most important hot-spots for common bean diversity in western-Himalayas. The analysis of genotypic data of SSR markers revealed a total of 263 alleles with an average of 10.52 alleles per locus. The genetic diversity analysis revealed higher variability in bean landraces belonging to Jammu region (He = 0.73) as compared to genotypes from Kashmir region (He = 0.647) and some exotic genotypes (0.71). The genotypes were also phenotyped for four important nutritional traits and the analysis of trait data revealed that sugar content was highest in common bean genotypes from Jammu region, while protein, starch and phenol content were highest in exotic common bean genotypes. Therefore, the superiority of common bean germplasm from Jammu region may be due to a higher level of allelic diversity, more private alleles and higher sugar content. The diverse genotypes based on genotypic data and trait performance will prove useful in future breeding programs aimed at enhancing nutritional contents of common bean varieties.
Keywords: common bean, genetic diversity, Jammu, Kashmir, nutritional traits, simple sequence repeats (SSRs), sugar content, Western Himalayas.
References
Angioi SA, Rau D, Rodriguez M, Logozzo G, Desiderio F, Papa R, Attene G (2009) Nuclear and chloroplast microsatellite diversity in Phaseolus vulgaris L. from Sardinia (Italy). Molecular Breeding 23, 413–429.| Nuclear and chloroplast microsatellite diversity in Phaseolus vulgaris L. from Sardinia (Italy).Crossref | GoogleScholarGoogle Scholar |
Bellucci E, Bitocchi E, Rau D, Rodriguez M, Biagetti E, Giardini A, Attene G, Nanni L, Papa R (2014) Genomics of origin, domestication and evolution of Phaseolus vulgaris. In ‘Genomics of plant genetic resources’. (Eds R Tuberosa, A Graner, E Frison) pp. 483–507. (Springer: Dordrecht).
| Crossref |
Bitocchi E, Bellucci E, Giardini A, Rau D, Rodriguez M, Biagetti E, Santilocchi R, Spagnoletti Zeuli P, Gioia T, Logozzo G, Attene G, Nanni L, Papa R (2013) Molecular analysis of the parallel domestication of the common bean (Phaseolus vulgaris) in Mesoamerica and the Andes. New Phytologist 197, 300–313.
| Molecular analysis of the parallel domestication of the common bean (Phaseolus vulgaris) in Mesoamerica and the Andes.Crossref | GoogleScholarGoogle Scholar |
Blair MW, Giraldo MC, Buendía HF, Tovar E, Duque MC, Beebe SE (2006) Microsatellite marker diversity in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 113, 100–109.
| Microsatellite marker diversity in common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar | 16614831PubMed |
Blair MW, Torres MM, Giraldo MC, Pedraza F (2009) Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.). BMC Plant Biology 9, 100
| Development and diversity of Andean-derived, gene-based microsatellites for common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar | 19646251PubMed |
Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72, 248–254.
| A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.Crossref | GoogleScholarGoogle Scholar | 942051PubMed |
Bray HG, Thorpe WV (1954) Analysis of phenolic compounds of interest in metabolism. Methods of Biochemical Analysis 1, 27–52.
| Analysis of phenolic compounds of interest in metabolism.Crossref | GoogleScholarGoogle Scholar | 13193524PubMed |
Broughton WJ, Hernandez G, Blair MW, Beebe S, Gepts P, Vanderleyden J (2003) Beans (Phaseolus spp.) – model food legumes. Plant and Soil 252, 55–128.
| Beans (Phaseolus spp.) – model food legumes.Crossref | GoogleScholarGoogle Scholar |
Buah S, Buruchara R, Okori P (2017) Molecular characterization of common bean (Phaseolus vulgaris L.) accessions from Southwestern Uganda reveal high levels of genetic diversity. Genetic Resources and Crop Evolution 64, 1985–1998.
| Molecular characterization of common bean (Phaseolus vulgaris L.) accessions from Southwestern Uganda reveal high levels of genetic diversity.Crossref | GoogleScholarGoogle Scholar |
Burle ML, Fonseca JR, Kami JA, Gepts P (2010) Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity. Theoretical and Applied Genetics 121, 801–813.
| Microsatellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity.Crossref | GoogleScholarGoogle Scholar | 20502861PubMed |
Caicedo AL, Gaitan E, Duque MC, Toro Chica O, Debouck DG, Thome J (1999) AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America. Crop Science 39, 1497–1507.
| AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America.Crossref | GoogleScholarGoogle Scholar |
Carović-Stanko K, Liber Z, Vidak M, Barešić A, Grdiša M, Lazarević B, Šatović Z (2017) Genetic diversity of Croatian common bean landraces. Frontiers in Plant Science 8, 604
| Genetic diversity of Croatian common bean landraces.Crossref | GoogleScholarGoogle Scholar | 28473842PubMed |
Castro-Guerrero NA, Isidra-Arellano MC, Mendoza-Cozatl DG, Valdés-López O (2016) Common bean: a legume model on the rise for unraveling responses and adaptations to iron, zinc, and phosphate deficiencies. Frontiers in Plant Science 7, 600
| Common bean: a legume model on the rise for unraveling responses and adaptations to iron, zinc, and phosphate deficiencies.Crossref | GoogleScholarGoogle Scholar | 27200068PubMed |
Choudhary N, Hamid A, Singh B, Khandy I, Sofi PA, Bhat MA, Mir RR (2018a) Insight into the origin of common bean (Phaseolus vulgaris L.) grown in the state of Jammu and Kashmir of north-western Himalayas. Genetic Resources and Crop Evolution 65, 963–977.
| Insight into the origin of common bean (Phaseolus vulgaris L.) grown in the state of Jammu and Kashmir of north-western Himalayas.Crossref | GoogleScholarGoogle Scholar |
Choudhary N, Bawa V, Paliwal R, Singh B, Bhat MA, Mir JI, Gupta M, Sofi PA, Thudi M, Varshney RK, Mir RR (2018b) Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas. PLoS ONE 13, e0191700
| Gene/QTL discovery for Anthracnose in common bean (Phaseolus vulgaris L.) from North-western Himalayas.Crossref | GoogleScholarGoogle Scholar | 29389971PubMed |
Chung HJ, Liu Q, Peter Pauls K, Fan MZ, Yada R (2008) In vitro starch digestibility, expected glycemic index and some physicochemical properties of starch and flour from common bean (Phaseolus vulgaris L.) varieties grown in Canada. Food Research International 41, 869–875.
| In vitro starch digestibility, expected glycemic index and some physicochemical properties of starch and flour from common bean (Phaseolus vulgaris L.) varieties grown in Canada.Crossref | GoogleScholarGoogle Scholar |
Cortes AJ, Chavarro MC, Blair MW (2011) SNP marker diversity in common bean (Phaseolus vulgaris L.). Theoretical and Applied Genetics 123, 827–845.
| SNP marker diversity in common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar | 21785951PubMed |
Diapari M, Sindhu A, Bett K, Deokar A, Warkentin TD, Tar’an B (2014) Genetic diversity and association mapping of iron and zinc concentrations in chickpea (Cicer arietinum L.). Genome 57, 459–468.
| Genetic diversity and association mapping of iron and zinc concentrations in chickpea (Cicer arietinum L.).Crossref | GoogleScholarGoogle Scholar | 25434748PubMed |
Díaz LM, Blair MW (2006) Race structure within the Mesoamerican gene pool of common bean (Phaseolus vulgaris L.) as determined by microsatellite markers. Theoretical and Applied Genetics 114, 143–154.
| Race structure within the Mesoamerican gene pool of common bean (Phaseolus vulgaris L.) as determined by microsatellite markers.Crossref | GoogleScholarGoogle Scholar | 17047911PubMed |
Díaz LM, Buendía HF, Duque MC, Blair MW (2011) Genetic diversity of Colombian landraces of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites. Plant Genetic Resources 9, 86–96.
| Genetic diversity of Colombian landraces of common bean as detected through the use of silver-stained and fluorescently labelled microsatellites.Crossref | GoogleScholarGoogle Scholar |
Duarte JM, Santos JBD, Melo LC (1999) Genetic divergence among common bean cultivars from different races based on RAPD markers. Genetics and Molecular Biology 22, 419–426.
| Genetic divergence among common bean cultivars from different races based on RAPD markers.Crossref | GoogleScholarGoogle Scholar |
DuBois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Analytical Chemistry 28, 350–356.
| Colorimetric method for determination of sugars and related substances.Crossref | GoogleScholarGoogle Scholar |
Fan G, Beta T (2016) Proximate composition, phenolic profiles and antioxidant capacity of three common bean varieties (Phaseolus vulgaris L.). Journal of Food Chemistry and Nutrition 2, 147–152.
| Proximate composition, phenolic profiles and antioxidant capacity of three common bean varieties (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Fayaz H, Mir AH, Tyagi S, Wani AA, Jan N, Yasin M, Mir JI, Mondal B, Khan MA, Mir RR (2021) Assessment of molecular genetic diversity of 384 chickpea genotypes anddevelopment of core set of 192 genotypes for chickpea improvementprograms. Genetic Resources and Crop Evolution
| Assessment of molecular genetic diversity of 384 chickpea genotypes anddevelopment of core set of 192 genotypes for chickpea improvementprograms.Crossref | GoogleScholarGoogle Scholar |
Galván MZ, Menéndez-Sevillano MC, De Ron AM, Santalla M, Balatti PA (2006) Genetic diversity among wild common beans from northwestern Argentina based on morpho agronomic and RAPD data. Genetic Resources and Crop Evolution 53, 891–900.
| Genetic diversity among wild common beans from northwestern Argentina based on morpho agronomic and RAPD data.Crossref | GoogleScholarGoogle Scholar |
Gepts P (1998) Origin and evolution of common bean: past events and recent trends. HortScience 33, 1124–1130.
| Origin and evolution of common bean: past events and recent trends.Crossref | GoogleScholarGoogle Scholar |
Gepts P, Bliss FA (1985) F1-hybrid weakness in the common bean: differential geographic origin suggests two gene pools in cultivated bean germplasm. Journal of Heredity 76, 447–450.
| F1-hybrid weakness in the common bean: differential geographic origin suggests two gene pools in cultivated bean germplasm.Crossref | GoogleScholarGoogle Scholar |
Gepts P, Debouck D (1991) Origin, domestication, and evolution of the common bean (Phaseolus vulgaris L.). In ‘Common beans: research for crop improvement’. (Eds A van Schoonhoven, O Voysest) pp. 7–537. (CAB, CIAT: Wallingford, UK, Cali, Colombia)
Gepts P, Osborn TC, Rashka K, Bliss FA (1986) Phaseolin-protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple centers of domestication. Economic Botany 40, 451–468.
| Phaseolin-protein variability in wild forms and landraces of the common bean (Phaseolus vulgaris): evidence for multiple centers of domestication.Crossref | GoogleScholarGoogle Scholar |
Gioia T, Logozzo G, Marzario S, Spagnoletti Zeuli P, Gepts P (2019) Evolution of SSR diversity from wild types to US advanced cultivars in the Andean and Mesoamerican domestications of common bean (Phaseolus vulgaris). PLoS ONE 14, e0211342
| Evolution of SSR diversity from wild types to US advanced cultivars in the Andean and Mesoamerican domestications of common bean (Phaseolus vulgaris).Crossref | GoogleScholarGoogle Scholar | 30703134PubMed |
Göl Ş, Doğanlar S, Frary A (2017) Relationship between geographical origin, seed size and genetic diversity in faba bean (Vicia faba L.) as revealed by SSR markers. Molecular Genetics and Genomics 292, 991–999.
| Relationship between geographical origin, seed size and genetic diversity in faba bean (Vicia faba L.) as revealed by SSR markers.Crossref | GoogleScholarGoogle Scholar | 28497168PubMed |
Gómez O, Blair MW, Frankow-Lindberg B, Gullberg U (2004) Molecular and phenotypic diversity of common bean landraces from Nicaragua. Crop Science 44, 1412–1418.
| Molecular and phenotypic diversity of common bean landraces from Nicaragua.Crossref | GoogleScholarGoogle Scholar |
Gouveia CSS, Freitas G, Pinheiro de Carvalho MAA (2011) Nutritional analysis of regional varieties of common bean (Phaseolus vulgaris L.) produced under low input conditions. Actas Portuguesas de Horticultura 17, 131–137.
Gupta PK, Rustgi S, Mir RR (2008) Array-based high-throughput DNA markers for crop improvement. Heredity 101, 5–18.
| Array-based high-throughput DNA markers for crop improvement.Crossref | GoogleScholarGoogle Scholar | 18461083PubMed |
Gupta PK, Rustgi S, Mir RR (2013) Array-based high-throughput DNA markers and genotyping platforms for cereal genetics and genomics. In ‘Cereal genomics II’. (Eds PK Gupta, RK Varshney). (Springer: Dordrecht, The Netherlands).
| Crossref |
Hodge JE, Hofreiter BT (1962) Determination of reducing sugars and carbohydrates. In ‘Methods in carbohydrate chemistry’. (Eds RL Whistler, ML Wolfrom) pp. 380–394. (Academic Press, New York)
Hughes T, Hoover R, Liu Q, Donner E, Chibbar R, Jaiswal S (2009) Composition, morphology, molecular structure, and physicochemical properties of starches from newly released chickpea (Cicer arietinum L.) cultivars grown in Canada. Food Research International 42, 627–635.
| Composition, morphology, molecular structure, and physicochemical properties of starches from newly released chickpea (Cicer arietinum L.) cultivars grown in Canada.Crossref | GoogleScholarGoogle Scholar |
Jan S, Khan MN, Jan S, Zaffar A, Rashid R, Khan MA, Sheikh FA, Bhat MA, Mir RR (2021a) Trait phenotyping and molecular marker characterization of barley (Hordeum vulgare L.) germplasm from Western Himalayas. Genetic Resources and Crop Evolution
| Trait phenotyping and molecular marker characterization of barley (Hordeum vulgare L.) germplasm from Western Himalayas.Crossref | GoogleScholarGoogle Scholar |
Jan S, Rather IA, Sofi PA, Wani MA, Sheikh FA, Bhat MA, Mir RR (2021b) Characterization of common bean (Phaseolus vulgaris L.) germplasm for morphological and seed nutrient traits from Western Himalayas. Legume Science 3, e86
| Characterization of common bean (Phaseolus vulgaris L.) germplasm for morphological and seed nutrient traits from Western Himalayas.Crossref | GoogleScholarGoogle Scholar |
Jha AB, Ashok Kumar K, Diapari M, Ambrose SJ, Zhang H, Tar’an B, Bett KE, Vandenberg A, Warkentin TD, Purves RW (2015) Genetic diversity of folate profiles in seeds of common bean, lentil, chickpea and pea. Journal of Food Composition and Analysis 42, 134–140.
| Genetic diversity of folate profiles in seeds of common bean, lentil, chickpea and pea.Crossref | GoogleScholarGoogle Scholar |
Kigel J (1999) Culinary and nutritional quality of Phaseolus vulgaris seeds as affected by environmental factors. Biotechnology, Agronomy, Society and Environment 3, 205–209.
Kozlowska H (2001) Nutrition. In ‘Carbohydrates in grain legume seeds’. (Ed. CL Hedley) pp. 61–67. (CAB International)
Kumar S, Kumar M, Mir RR, Kumar R, Kumar S (2021) Advances in molecular markers and their use in genetic improvement of wheat. In ‘Physiological, molecular, and genetic perspectives of wheat improvement’. (Eds SH Wani, A Mohan, GP Singh) pp. 139–174. (Springer Nature)
Kumar V, Sharma S, Sharma AK, Sharma S, Bhat KV (2009) Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean. Euphytica 170, 249–262.
| Comparative analysis of diversity based on morpho-agronomic traits and microsatellite markers in common bean.Crossref | GoogleScholarGoogle Scholar |
Kwak M, Gepts P (2009) Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae). Theoretical and Applied Genetics 118, 979–992.
| Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L., Fabaceae).Crossref | GoogleScholarGoogle Scholar | 19130029PubMed |
Maciel FL, Echeverrigaray S, Gerald LTS, Grazziotin FG (2003) Genetic relationships and diversity among Brazilian cultivars and land races of common beans (Phaseolus vulgaris L.) revealed by AFLP markers. Genetic Resources and Crop Evolution 50, 887–893.
| Genetic relationships and diversity among Brazilian cultivars and land races of common beans (Phaseolus vulgaris L.) revealed by AFLP markers.Crossref | GoogleScholarGoogle Scholar |
Maciel FL, Gerald LTS, Echeverrigaray S (2001) Random amplified polymorphic DNA (RAPD) markers variability among cultivars and landraces of common beans (Phaseolus vulgaris L.) of south-Brazil. Euphytica 120, 257–263.
| Random amplified polymorphic DNA (RAPD) markers variability among cultivars and landraces of common beans (Phaseolus vulgaris L.) of south-Brazil.Crossref | GoogleScholarGoogle Scholar |
Mahadevamma S, Shamala TR, Tharanathan RN (2004) Resistant starch derived from processed legumes: in vitro and in vivo fermentation characteristics. International Journal of Food Science and Nutrition 55, 399–405.
| Resistant starch derived from processed legumes: in vitro and in vivo fermentation characteristics.Crossref | GoogleScholarGoogle Scholar |
Maras M, Sušnik S, Šuštar-Vozlič J, Meglič V (2006) Temporal changes in genetic diversity of common bean (Phaseolus vulgaris L.) accessions cultivated between 1800 and 2000. Russian Journal of Genetics 42, 775–782.
| Temporal changes in genetic diversity of common bean (Phaseolus vulgaris L.) accessions cultivated between 1800 and 2000.Crossref | GoogleScholarGoogle Scholar |
Masi P, Spagnoletti Zeuli PL, Donini P (2003) Development and analysis of multiplex microsatellite markers sets in common bean (Phaseolus vulgaris L.). Molecular Breeding 11, 303–313.
| Development and analysis of multiplex microsatellite markers sets in common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Mir RR, Hiremath PJ, Riera-Lizarazu O, Varshney RK (2013) Evolving molecular marker technologies in plants: from RFLPs to GBS. In ‘Diagnostics in plant breeding’. (Eds T Lübberstedt, RK Varshney) pp. 229–247. (Springer: New York)
Mir RR, Varshney RK (2013) Future prospects of molecular markers in plants. In ‘Molecular markers in plants’. (Ed. RJ Henry) pp. 169–190. (Blackwell Publishing: Oxford, UK)
Mir RR, Choudhary N, Bawa V, Jan S, Singh B, Bhat MA, Paliwal R, Kumar A, Chitikineni A, Thudi M, Varshney RK (2021) Allelic diversity, structural analysis and genome-wide association study (GWAS) for yield and related traits using unexplored common bean (Phaseolus vulgaris L.) germplasm from Western Himalayas. Frontiers in Genetics 11, 609603
| Allelic diversity, structural analysis and genome-wide association study (GWAS) for yield and related traits using unexplored common bean (Phaseolus vulgaris L.) germplasm from Western Himalayas.Crossref | GoogleScholarGoogle Scholar | 33584807PubMed |
Mir RR, Kumar J, Balyan HS, Gupta PK (2012) A study of genetic diversity among Indian bread wheat (Triticum aestivum L.) cultivars released during last 100 years. Genetic Resources and Crop Evolution 59, 717–726.
| A study of genetic diversity among Indian bread wheat (Triticum aestivum L.) cultivars released during last 100 years.Crossref | GoogleScholarGoogle Scholar |
Nkhata W, Shimelis H, Melis R, Chirwa R, Mzengeza T, Mathew I, Shayanowako A (2020) Population structure and genetic diversity analyses of common bean germplasm collections of East and Southern Africa using morphological traits and high-density SNP markers. PLoS ONE 15, e0243238
| Population structure and genetic diversity analyses of common bean germplasm collections of East and Southern Africa using morphological traits and high-density SNP markers.Crossref | GoogleScholarGoogle Scholar | 33338076PubMed |
Nyombaire G, Siddiq M, Dolan K (2007) Effect of soaking and cooking on the oligosaccharides and lectins of red kidney beans (Phaseolus vulgaris L.). Annual Report of the Bean Improvement Cooperative 50, 31–32.
Pedrosa-Harand A, Porch T, Gepts P (2008) Standard nomenclature for common bean chromosomes and linkage groups. Annual Report of the Bean Improvement Cooperative 51, 106
Pérez-Vega E, Campa A, De la Rosa L, Giraldez R, Ferreira JJ (2009) Genetic diversity in a core collection established from the main bean genebank in Spain. Crop Science 49, 1377–1386.
| Genetic diversity in a core collection established from the main bean genebank in Spain.Crossref | GoogleScholarGoogle Scholar |
Pinon MGT, Moghaddam SM, Lee RK, Mérida JCV, DeYoung DJ, Reyes BA, Maredia M, Osorno JM, McClean PE (2021) Genetic diversity of Guatemalan climbing bean collections. Genetic Resources and Crop Evolution 68, 639–656.
| Genetic diversity of Guatemalan climbing bean collections.Crossref | GoogleScholarGoogle Scholar |
Pujolà M, Farreras A, Casañas F (2007) Protein and starch content of raw, soaked and cooked beans (Phaseolus vulgaris L.). Food Chemistry 102, 1034–1041.
| Protein and starch content of raw, soaked and cooked beans (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Rivera A, Plans M, Sabaté J, Casañas F, Casals J, Rull A, Simó J (2018) The Spanish core collection of common beans (Phaseolus vulgaris L.): an important source of variability for breeding chemical composition. Frontiers in Plant Science 9, 1642
| The Spanish core collection of common beans (Phaseolus vulgaris L.): an important source of variability for breeding chemical composition.Crossref | GoogleScholarGoogle Scholar | 30483294PubMed |
Saba I, Sofi PA, Zeerak NA, Bhat MA, Mir RR (2016) Characterisation of a core set of common bean (Phaseolus vulgaris L.) germplasm for seed quality traits. SABRAO Journal of Breeding and Genetics 48, 359–376.
Saba I, Sofi PA, Zeerak NA, Mir RR, Gul M (2017) Using augmented design for evaluation of common bean (Phaseolus vulgaris L.) germplasm. International Journal of Current Microbiology and Applied Science 6, 246–254.
| Using augmented design for evaluation of common bean (Phaseolus vulgaris L.) germplasm.Crossref | GoogleScholarGoogle Scholar |
Šajgalík M, Ondreičková K, Hauptvogel P, Mihálik D, Glasa M, Kraic J (2019) Higher effectiveness of new common bean (Phaseolus vulgaris L.) germplasm acquisition by collecting expeditions associated with molecular analyses. Sustainability 11, 5270
| Higher effectiveness of new common bean (Phaseolus vulgaris L.) germplasm acquisition by collecting expeditions associated with molecular analyses.Crossref | GoogleScholarGoogle Scholar |
Shafi S, Tahir M, Khan MA, Bhat MA, Kumar U, Kumar S, Mir RR (2021) Trait phenotyping and genic/random SSR markers characterization for breeding early maturing wheats from Western-Himalayas. Genetic Resources and Crop Evolution
| Trait phenotyping and genic/random SSR markers characterization for breeding early maturing wheats from Western-Himalayas.Crossref | GoogleScholarGoogle Scholar |
Sharma PN, Díaz LM, Blair MW (2013) Genetic diversity of two Indian common bean germplasm collections based on morphological and microsatellite markers. Plant Genetic Resources 11, 121–130.
| Genetic diversity of two Indian common bean germplasm collections based on morphological and microsatellite markers.Crossref | GoogleScholarGoogle Scholar |
Sihag P, Sagwal V, Kumar A, Balyan P, Mir RR, Dhankher OP, Kumar U (2021) Discovery of miRNAs and development of heat-responsive miRNA-SSR markers for characterization of wheat germplasm for terminal heat tolerance breeding. Frontiers in Genetics 12, 699420
| Discovery of miRNAs and development of heat-responsive miRNA-SSR markers for characterization of wheat germplasm for terminal heat tolerance breeding.Crossref | GoogleScholarGoogle Scholar | 34394189PubMed |
Singh SP (2001) Broadening the genetic base of common bean cultivars: a review. Crop Science 41, 1659–1675.
| Broadening the genetic base of common bean cultivars: a review.Crossref | GoogleScholarGoogle Scholar |
Smýkal P, Coyne CJ, Ambrose MJ, Maxted N, Schaefer H, Blair MW, Berger J, Greene SL, Nelson MN, Besharat N, Vymyslický T, Toker C, Saxena RK, Roorkiwal M, Pandey MK, Hu J, Li YH, Wang LX, Guo Y, Qiu LJ, Redden RJ, Varshney RK, Vymyslický T (2015) Legume crops phylogeny and genetic diversity for science and breeding. Critical Reviews in Plant Sciences 34, 43–104.
| Legume crops phylogeny and genetic diversity for science and breeding.Crossref | GoogleScholarGoogle Scholar |
Stoilova T, Pereira G, de Sousa MM, Carnide V (2005) Diversity in common bean landraces (Phaseolus vulgaris L.) from Bulgaria and Portugal. Journal of Central European Agriculture 6, 443–448.
Šustar-Vozlič J, Maras M, Javornik B, Meglič V (2006) Genetic diversity and origin of slovene common bean (Phaseolus vulgaris L.) germplasm as revealed by AFLP markers and phaseolin analysis. Journal of the American Society for Horticultural Science 131, 242–249.
| Genetic diversity and origin of slovene common bean (Phaseolus vulgaris L.) germplasm as revealed by AFLP markers and phaseolin analysis.Crossref | GoogleScholarGoogle Scholar |
Tahir M, Shafi S, Khan MA, Sheikh FA, Bhat MA, Sofi PA, Kumar S, Wani MA, Mir RR (2021) Grain micronutrient evaluation of wheat (Triticum aestivum L.) germplasm and molecular characterisation via genic and random SSR markers. Crop and Pasture Science
| Grain micronutrient evaluation of wheat (Triticum aestivum L.) germplasm and molecular characterisation via genic and random SSR markers.Crossref | GoogleScholarGoogle Scholar |
Tegelstrom H (1992) Detection of mitochondrial DNA fragments. In ‘Molecular genetic analysis of populations: a practical approach’. (Ed. AR Hoelzel) pp. 89–113. (IRL Press: Oxford)
Tigist SG, Melis R, Sibiya J, Amelework BA, Keneni G, Tegene A (2020) Genetic diversity analysis of common bean (Phaseolus vulgaris L.) genotypes for resistance to Mexican bean weevil (Zabrotes subfa sciatus), using single nucleotide polymorphism and phenotypic markers. Acta Agriculturae Scandinavica, Section B – Soil and Plant Science 70, 495–506.
| Genetic diversity analysis of common bean (Phaseolus vulgaris L.) genotypes for resistance to Mexican bean weevil (Zabrotes subfa sciatus), using single nucleotide polymorphism and phenotypic markers.Crossref | GoogleScholarGoogle Scholar |
Tyagi S, Sharma S, Ganie SA, Tahir M, Mir RR, Pandey R (2019) Plant microRNAs: biogenesis, gene silencing, web-based analysis tools and their use as molecular markers. 3 Biotech 9, 413
| Plant microRNAs: biogenesis, gene silencing, web-based analysis tools and their use as molecular markers.Crossref | GoogleScholarGoogle Scholar | 31696018PubMed |
Tyagi S, Kumar A, Gautam T, Pandey R, Rustgi S, Mir RR (2021) Development and use of miRNA-derived SSR markers for the study of genetic diversity, population structure, and characterization of genotypes for breeding heat tolerant wheat varieties. PLoS ONE 16, e0231063
| Development and use of miRNA-derived SSR markers for the study of genetic diversity, population structure, and characterization of genotypes for breeding heat tolerant wheat varieties.Crossref | GoogleScholarGoogle Scholar | 33539339PubMed |
VandenLangenberg KM, Bethke PC, Nienhuis J (2012) Patterns of fructose, glucose, and sucrose accumulation in snap and dry bean (Phaseolus vulgaris) pods. HortScience 47, 874–878.
| Patterns of fructose, glucose, and sucrose accumulation in snap and dry bean (Phaseolus vulgaris) pods.Crossref | GoogleScholarGoogle Scholar |
Vidak M, Carović-Stanko K, Barešić A, Grdiša M, Šatović Z, Liber Z (2017) Microsatellite markers in common bean (Phaseolus vulgaris L.). Journal of Central European Agriculture 18, 902–917.
| Microsatellite markers in common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Wu D, Hought J, Baseggio M, Hart JP, Gore MA, Ilut DC (2019) Genomic characterization of the native seeds/search common bean (Phaseolus vulgaris L.) collection and its seed coat patterns. Genetic Resources and Crop Evolution 66, 1469–1482.
| Genomic characterization of the native seeds/search common bean (Phaseolus vulgaris L.) collection and its seed coat patterns.Crossref | GoogleScholarGoogle Scholar |
Xu S, Wang G, Mao W, Hu Q, Liu N, Ye L, Gong Y (2014) Genetic diversity and population structure of common bean (Phaseolus vulgaris) landraces from China revealed by a new set of EST-SSR markers. Biochemical Systematics and Ecology 57, 250–256.
| Genetic diversity and population structure of common bean (Phaseolus vulgaris) landraces from China revealed by a new set of EST-SSR markers.Crossref | GoogleScholarGoogle Scholar |
Yu K, Park SJ, Poysa V, Gepts P (2000) Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.). Journal of Heredity 91, 429–434.
| Integration of simple sequence repeat (SSR) markers into a molecular linkage map of common bean (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Zargar SM, Farhat S, Mahajan R, Bhakhri A, Sharma A (2016) Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean. Saudi Journal of Biological Sciences 23, 139–149.
| Unraveling the efficiency of RAPD and SSR markers in diversity analysis and population structure estimation in common bean.Crossref | GoogleScholarGoogle Scholar | 26858551PubMed |
Zhang X, Blair MW, Wang S (2008) Genetic diversity of Chinese common bean (Phaseolus vulgaris L.) landraces assessed with simple sequence repeat (SSR) markers. Theoretical and Applied Genetics 117, 629–640.
| Genetic diversity of Chinese common bean (Phaseolus vulgaris L.) landraces assessed with simple sequence repeat (SSR) markers.Crossref | GoogleScholarGoogle Scholar | 18548226PubMed |
