Transcriptome-based discovery of genes and networks related to RSC3Q-mediated resistance to Soybean mosaic virus in soybean
Yuan Yuan A B , Yongqing Yang A , Jinlong Yin A , Yingchao Shen A , Bowen Li A , LiLiqun Wang A and Haijian Zhi
A C
+ Author Affiliations
- Author Affiliations
A National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Soybean, Ministry of Agriculture, Nanjing Agricultural University, Weigang 1, Nanjing 210095, People’s Republic of China.
B Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, People’s Republic of China.
C Corresponding author. Email: zhj@njau.edu.cn
Crop and Pasture Science 71(12) 987-995 https://doi.org/10.1071/CP20253
Submitted: 18 July 2020 Accepted: 14 October 2020 Published: 7 December 2020
Journal Compilation © CSIRO 2020 Open Access CC BY-NC-ND
Abstract
Soybean mosaic virus (SMV) is a worldwide disease of soybean (Glycine max (L.) Merr.) that can cause serious reduction in yield and seed quality. Soybean cv. Qihuang-1 is an important source of resistance to SMV in China, carrying a resistance gene (RSC3Q) against SMV strain SC3. In order to discover genes and networks regulated by RSC3Q-mediated resistance in Qihuang-1, we analysed transcriptome data of a pair of near-isogenic lines, R (RSC3Q) and S (rSC3Q), from the cross Qihuang-1 × Nannong 1138-2 (rSC3Q), after SC3 inoculation. Many differentially expressed genes (DEGs) were identified in the R and S lines at 6, 20 and 48 h post-inoculation. Based on pathway-enrichment analysis of DEGs, three genes encoding calmodulin-like protein (Glyma03g28650, Glyma19g31395 and Glyma11g33790) with downregulated expression in the S line were identified in the plant–pathogen interaction pathway at 6 h post-inoculation. Analyses by quantitative real-time PCR were performed to verify that these three genes were not beneficial for SMV infection. Our results also revealed a complex plant-hormone signal network in RSC3Q-mediated resistance during the early stage of SMV infection. Expression of jasmonic acid repressor genes (TIFY/JAZ) and abscisic acid-induced genes (PP2C3a) was upregulated in the R line but not the S line. More DEGs related to indole-3-acetic acid were found in the R line than the S line, and no salicylic acid-related DEGs were identified. These results suggest that suppression of jasmonic acid or promotion of abscisic acid is important for RSC3Q-mediated resistance against SC3, and that salicylic acid may not act as a main regulator of RSC3Q-mediated resistance during early stages of SC3 infection. Growth and development were greatly affected through RSC3Q-mediated resistance responses after SC3 infection. Our understanding would be enhanced by identification of factors associated with RSC3Q that help to trigger the resistance response.
Keywords: CML, Glycine max, NILs, plant-hormone signal-transduction pathway, Soybean mosaic virus.
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