Morpho-physiological adaptation and DNA methylation of wheat seedlings under osmotic stress
Jingyun Li A , Wenjing Jia A , Huihui Wang A , Yanqiu Zhu A , Zhikun Duan B , Lina Jiang A , Yanqing Zhou A C and Hongying Duan
A C
+ Author Affiliations
- Author Affiliations
A College of Life Sciences, Henan Normal University, Xinxiang, Henan 453007, China.
B College of Life Sciences, Henan University, Kaifeng, Henan 475004, China.
C Corresponding authors. Emails: dxdhy@163.com; yqzhou@htu.cn
Crop and Pasture Science 71(4) 349-355 https://doi.org/10.1071/CP19429
Submitted: 16 October 2019 Accepted: 17 March 2020 Published: 22 April 2020
Abstract
The quality and yield of wheat (Triticum aestivum L.) are dramatically affected by drought. We used morphological and physiological characteristics and degree of DNA methylation to compare the responses of two wheat cultivars under osmotic stress, and found that the two cultivars behaved differently. Root development, leaf growth, and the accumulation of proline and soluble carbohydrate in wheat cv. AK58 all showed drought tolerance. Drought tolerance of wheat cv. XM13 was mainly improved by accumulation of proline and soluble carbohydrate. The degree of DNA methylation in wheat showed tissue specificity and increased significantly in leaf tissue with increasing osmotic stress, but decreased significantly in root tissue under mild osmotic stress. In addition, changes of DNA methylation differed between two wheat cultivars under osmotic stress, and this change was especially significant in AK58. Therefore, wheat AK58 may have stronger self-adjustment ability under osmotic stress compared with XM13, and might respond more rapidly to osmotic stress through the change of DNA methylation. This finding could be significant for revealing drought-tolerance mechanisms of plants.
Additional keywords: epigenetic, osmotic stress, plant growth.
References
Amini H, Arzani A, Karami M (2014) Effect of water deficiency on seed quality and physiological traits of different safflower genotypes. Turkish Journal of Biology 38, 271–282.| Effect of water deficiency on seed quality and physiological traits of different safflower genotypes.Crossref | GoogleScholarGoogle Scholar |
Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA (1987) Current protocols in molecular biology. The Quarterly Review of Biology 1, 286
Bandurska H (2001) Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injuries? ii. proline accumulation during hardening and its involvement in reducing membrane injuries in leaves subjected to severe osmotic stress. Acta Physiologiae Plantarum 23, 483–490.
| Does proline accumulated in leaves of water deficit stressed barley plants confine cell membrane injuries? ii. proline accumulation during hardening and its involvement in reducing membrane injuries in leaves subjected to severe osmotic stress.Crossref | GoogleScholarGoogle Scholar |
Bhargava S, Sawant K (2013) Drought stress adaptation: metabolic adjustment and regulation of gene expression. Plant Breeding 132, 21–32.
| Drought stress adaptation: metabolic adjustment and regulation of gene expression.Crossref | GoogleScholarGoogle Scholar |
Candaele J, Demuynck K, Mosoti D, Beemster GTS, Beemster D, Nelissen H (2014) Differential methylation during maize leaf growth targets developmentally regulated genes. Plant Physiology 164, 1350–1364.
| Differential methylation during maize leaf growth targets developmentally regulated genes.Crossref | GoogleScholarGoogle Scholar | 24488968PubMed |
Carvalho P, Azam-Ali S, Foulkes MJ (2014) Quantifying relationships between rooting traits and water uptake under drought in Mediterranean barley and durum wheat. Journal of Integrative Plant Biology 56, 455–469.
| Quantifying relationships between rooting traits and water uptake under drought in Mediterranean barley and durum wheat.Crossref | GoogleScholarGoogle Scholar | 24112696PubMed |
Chen X, Hu J, Zhang H, Ding Y (2014) DNA methylation changes in photoperiod-thermo-sensitive male sterile rice pa64s under two different conditions. Gene 537, 143–148.
| DNA methylation changes in photoperiod-thermo-sensitive male sterile rice pa64s under two different conditions.Crossref | GoogleScholarGoogle Scholar | 24365594PubMed |
Christmann A, Weiler EW, Steudle E, Grill E (2007) A hydraulic signal in root-to-shoot signalling of water shortage. The Plant Journal 52, 167–174.
| A hydraulic signal in root-to-shoot signalling of water shortage.Crossref | GoogleScholarGoogle Scholar | 17711416PubMed |
Dowen RH, Ecker JR (2012) Widespread dynamic DNA methylation in response to biotic stress. Proceedings of the National Academy of Sciences of the United States of America 109, E2183–E2191.
| Widespread dynamic DNA methylation in response to biotic stress.Crossref | GoogleScholarGoogle Scholar | 22733782PubMed |
Duan HY, Liu WX, Li JY, Ding WK, Zhu YQ, Wang HN (2016) Growth and DNA methylation level of Triticum aestivum seedlings treated with 5-azacytidine. Pakistan Journal of Botany 48, 1585–1591.
Duan HY, Li JJ, Wang HN, Chen FF, Ding WK, Zhu YQ, Zheng X, Zeng YP, Zhou YQ (2018) Effects of DNA methylation on growth and development of Rehmannia glutinosa. International Journal of Agriculture and Biology 20, 2161–2168.
Fan HH, Li TC, Guan L, Li ZP, Guo N, Cai YP (2012) Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense, grown under drought stress. Plant, Cell, Tissue & Organ Culture 109, 307–314.
| Effects of exogenous nitric oxide on antioxidation and DNA methylation of Dendrobium huoshanense, grown under drought stress.Crossref | GoogleScholarGoogle Scholar |
Fei Y, Xue Y, Du P, Yang S, Deng X (2017) Expression analysis and promoter methylation under osmotic and salinity stress of TaGAPC1 in wheat (Triticum aestivum L). Protoplasma 254, 987–996.
| Expression analysis and promoter methylation under osmotic and salinity stress of TaGAPC1 in wheat (Triticum aestivum L).Crossref | GoogleScholarGoogle Scholar | 27488925PubMed |
Gohlke J, Scholz CJ, Kneitz S, Weber D, Fuchs J, Hedrich R, Deeken R (2013) DNA methylation mediated control of gene expression is critical for development of crown gall tumors. PLOS Genetics 9, e1003267
| DNA methylation mediated control of gene expression is critical for development of crown gall tumors.Crossref | GoogleScholarGoogle Scholar | 23408907PubMed |
Hansen J, Moller I (1975) Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone. Analytical Biochemistry 68, 87–94.
| Percolation of starch and soluble carbohydrates from plant tissue for quantitative determination with anthrone.Crossref | GoogleScholarGoogle Scholar | 1190454PubMed |
Hua ZR, Li XL (2011) Effect of PEG treatment on seed germination and seedling growth of Shangluo Scutellaria baicalensis. Seed 30, 102–104.
Li KR, Feng CH (2011) Effects of brassinolide on drought resistance of Xanthoceras sorbifolia, seedlings under water stress. Acta Physiologiae Plantarum 33, 1293–1300.
| Effects of brassinolide on drought resistance of Xanthoceras sorbifolia, seedlings under water stress.Crossref | GoogleScholarGoogle Scholar |
Li X, Liu F (2014) Drought stress memory and drought stress tolerance in plants: biochemical and molecular basis. Drought Stress Tolerance in Plants 1, 17–44.
Li YY, Cheng P, Xiong XY, Hong YH (2012) DNA methylation in potato under drought stress. Chinese Potato 26, 11–16.
Marfil CF, Camadro EL, Masuelli RW (2009) Phenotypic instability and epigenetic variability in a diploid potato of hybrid origin, Solanum ruiz-lealii. BMC Plant Biology 9, 21
| Phenotypic instability and epigenetic variability in a diploid potato of hybrid origin, Solanum ruiz-lealii.Crossref | GoogleScholarGoogle Scholar | 19232108PubMed |
Mastan SG, Rathore MS, Bhatt VD, Yadav P, Chikara J (2012) Assessment of changes in DNA methylation by methylation-sensitive amplification polymorphism in Jatropha curcas L. subjected to salinity stress. Gene 508, 125–129.
| Assessment of changes in DNA methylation by methylation-sensitive amplification polymorphism in Jatropha curcas L. subjected to salinity stress.Crossref | GoogleScholarGoogle Scholar | 22967874PubMed |
Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiology 51, 914–916.
| The osmotic potential of polyethylene glycol 6000.Crossref | GoogleScholarGoogle Scholar | 16658439PubMed |
Migicovsky Z, Kovalchuk I (2013) Changes to DNA methylation and homologous recombination frequency in the progeny of stressed plants. Biochemistry and Cell Biology 91, 1–5.
| Changes to DNA methylation and homologous recombination frequency in the progeny of stressed plants.Crossref | GoogleScholarGoogle Scholar | 23442135PubMed |
Nakano K, Whitaker JW, Boyle DL, Wang W, Firestein GS (2013) DNA methylome signature in rheumatoid arthritis. Annals of the Rheumatic Diseases 72, 110–117.
| DNA methylome signature in rheumatoid arthritis.Crossref | GoogleScholarGoogle Scholar | 22736089PubMed |
Osabe K, Clement JD, Bedon F, Pettolino FA, Ziolkowski L, Llewellyn DJ (2014) Genetic and DNA methylation changes in cotton (Gossypium) genotypes and tissues. PLoS One 9, e86049
| Genetic and DNA methylation changes in cotton (Gossypium) genotypes and tissues.Crossref | GoogleScholarGoogle Scholar | 24465864PubMed |
Razin A, Friedman J (1981) DNA methylation and its possible biological roles. Progress in Nucleic Acid Research and Molecular Biology 25, 33–52.
| DNA methylation and its possible biological roles.Crossref | GoogleScholarGoogle Scholar | 6261297PubMed |
Rohit J, Wani SH, Balwant S, Abhishek B, Dar ZA, Lone AA (2016) Transcription factors and plants response to drought stress: current understanding and future directions. Frontiers in Plant Science 7, 1029
| Transcription factors and plants response to drought stress: current understanding and future directions.Crossref | GoogleScholarGoogle Scholar |
Rollins JA, Habte E, Templer SE, Colby T, Schmidt J, Korff MV (2013) Leaf proteome alterations in the context of physiological and morphological responses to drought and heat stress in barley (Hordeum vulgare L.). Journal of Experimental Botany 64, 3201–3212.
| Leaf proteome alterations in the context of physiological and morphological responses to drought and heat stress in barley (Hordeum vulgare L.).Crossref | GoogleScholarGoogle Scholar | 23918963PubMed |
Sabbah S, Raise M, Tal M (1995) Methylation of DNA in NaCl-adapted cells of potato. Plant Cell Reports 14, 467–470.
| Methylation of DNA in NaCl-adapted cells of potato.Crossref | GoogleScholarGoogle Scholar | 24185459PubMed |
Saha P, Sade N, Arzani A, Rubio Wilhelmi MDM, Coe KM, Li B, Blumwald E (2016) Effects of abiotic stress on physiological plasticity and water use of Setaria viridis (L.). Plant Science 251, 128–138.
| Effects of abiotic stress on physiological plasticity and water use of Setaria viridis (L.).Crossref | GoogleScholarGoogle Scholar | 27593471PubMed |
Shafiq S, Zeb Q, Ali A, Sajjad Y, Nazir R, Widemann E, Liu L (2019) Lead, cadmium and zinc phytotoxicity alter DNA methylation levels to confer heavy metal tolerance in wheat. International Journal of Molecular Sciences 20, 4676
| Lead, cadmium and zinc phytotoxicity alter DNA methylation levels to confer heavy metal tolerance in wheat.Crossref | GoogleScholarGoogle Scholar |
Shao HB, Chu LY, Jaleel CA, Zhao CX (2008) Water-deficit stress-induced anatomical changes in higher plants. Comptes Rendus Biologies 331, 215–225.
| Water-deficit stress-induced anatomical changes in higher plants.Crossref | GoogleScholarGoogle Scholar | 18280987PubMed |
Tang XM, Tao X, Wang Y, Ma DW, Li D, Yang H (2014) Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique. Molecular Genetics and Genomics 289, 1075–1084.
| Analysis of DNA methylation of perennial ryegrass under drought using the methylation-sensitive amplification polymorphism (MSAP) technique.Crossref | GoogleScholarGoogle Scholar | 24916310PubMed |
Tsaftaris AS, Polidoros AN (2000) DNA methylation and plant breeding. Plant Breeding Reviews 18, 87–176.
Tuteja N, Gill SS, Tuteja R (2011) Plant responses to abiotic stresses: shedding light on salt, drought, cold and heavy metal stress. In ‘Omics and plant abiotic stress tolerance’. pp. 39–64. (Bentham Science Publisher: Sharjah, UAE)
Wang WS, Pan YJ, Zhao XQ, Dwivedi D, Zhu LH, Ali J (2011) Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.). Journal of Experimental Botany 62, 1951–1960.
| Drought-induced site-specific DNA methylation and its association with drought tolerance in rice (Oryza sativa L.).Crossref | GoogleScholarGoogle Scholar | 21193578PubMed |
Wren JJ, Wiggall PH (1965) An improved colorimetric method for the determination of proline in the presence of other ninhydrin-positive compounds. The Biochemical Journal 94, 216–220.
| An improved colorimetric method for the determination of proline in the presence of other ninhydrin-positive compounds.Crossref | GoogleScholarGoogle Scholar | 14342233PubMed |
Yaish MW, Colasanti J, Rothstein SJ (2011) The role of epigenetic processes in controlling flowering time in plants exposed to stress. Journal of Experimental Botany 62, 3727–3735.
| The role of epigenetic processes in controlling flowering time in plants exposed to stress.Crossref | GoogleScholarGoogle Scholar | 21633082PubMed |
Yamada M, Morishita H, Urano K, Shiozaki N, Yamaguchi-Shinozaki K, Shinozaki K (2005) Effects of free proline accumulation in petunias under drought stress. Journal of Experimental Botany 56, 1975–1981.
| Effects of free proline accumulation in petunias under drought stress.Crossref | GoogleScholarGoogle Scholar | 15928013PubMed |
Zhao HC, Kang YH, Gong XC, Yin J, Tian ZM (2013) Effects of drought stress on physiological and biochemical indices of different potato variety. Crops 6, 63–69.
Zheng X, Chen L, Li M, Lou Q, Xia H, Wang P (2013) Transgenerational variations in DNA methylation induced by drought stress in two rice varieties with distinguished difference to drought resistance. PLoS One 8, e80253
| Transgenerational variations in DNA methylation induced by drought stress in two rice varieties with distinguished difference to drought resistance.Crossref | GoogleScholarGoogle Scholar | 24358345PubMed |
Zivcak M, Brestic M, Sytar O (2016) Osmotic adjustment and plant adaptation to drought stress. Drought Stress Tolerance in Plants 1, 105–143.
| Osmotic adjustment and plant adaptation to drought stress.Crossref | GoogleScholarGoogle Scholar |
