Microorganism profile, fermentation quality and rumen digestibility in vitro of maize-stalk silages produced at different maturity stages
Miao Zhang A , Yanping Wang A , Zhongfang Tan A C , Zongwei Li A , Ya Li A , Haoxin Lv A , Bei Zhang A and Qingsheng Jin B
+ Author Affiliations
- Author Affiliations
A Henan Provincial Key Laboratory of Ion Beam Bio-engineering, Zhengzhou University, Zhengzhou 450052, Henan Province, China.
B Institute of Crops and Utilisation of Nuclear Technology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, Zhejiang Province, China.
C Corresponding author. Email: tzhongfang@zzu.edu.cn
Crop and Pasture Science 68(3) 225-233 https://doi.org/10.1071/CP16324
Submitted: 8 August 2016 Accepted: 17 February 2017 Published: 22 March 2017
Abstract
This study aimed to investigate the microorganism profile, fermentation quality and rumen digestibility in vitro of maize-stalk silage at different maturity stages. Maize-stalk samples were harvested at the stages milk-ripe, dough, fully ripe, and fully ripe exposed to air for 3 or 10 days. Silage pH, ammonia-N and chemical composition were measured. Thirteen representative lactic acid bacteria (LAB) strains isolated from the raw materials were categorised into five profile clusters: Leuconostoc citreum (23.1%), Weissella paramesenteroides (15.4%), Lactococcus garvieae (23.1%), Enterococcus faecalis (7.7%), and Lactobacillus paraplantarum (30.8%). The total LAB numbers in silages with raw materials reaped at the stages milk-ripe, dough, fully ripe, and fully ripe exposed to air for 3 or 10 days, respectively, were approximately 8, 6, 10, 3.5 and 10 log CFU g–1. The dominant LAB types of maize-stalk silage at different stages were all different. The epiphytic pathogens Escherichia coli, aerobic bacteria, filamentous fungi and Saccharomycetes were found in silages of all stages. There were significant differences (P < 0.001) in crude protein, ether extract, dry matter, acid detergent fibre and organic matter of silage at different stages; however, no significant difference (P > 0.05) was observed in dry matter digestibility after 24 h of fermentation in vitro, with NH3-N varying from 0.7 ± 0.1 to 1.7 ± 0.3 mg L–1.
Additional keywords: cornstalk, ensilage, moisture, pathogen, ruminal fermentation.
References
Ali I, Fontenot JP, Allen VG (2009) Palatability and dry matter intake by sheep fed corn stover treated with different nitrogen sources. Pakistan Veterinary Journal 29, 199–201.Antonsson M, Molin G, Ardo Y (2003) Lactobacillus strains isolated from Danbo cheese as adjunct cultures in a cheese model system. International Journal of Food Microbiology 85, 159–169.
| Lactobacillus strains isolated from Danbo cheese as adjunct cultures in a cheese model system.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXksF2gurw%3D&md5=e001f8a2e8d6f3785448b03cc883875cCAS |
AOAC (1990) ‘Official methods of analysis.’ 15th edn. (Association of Official Analytical Chemists: Arlington, VA)
Ávila CL, Carvalho BF, Pinto JC, Duarte WF, Schwan RF (2014) The use of Lactobacillus species as starter cultures for enhancing the quality of sugar cane silage. Journal of Dairy Science 97, 940–951.
| The use of Lactobacillus species as starter cultures for enhancing the quality of sugar cane silage.Crossref | GoogleScholarGoogle Scholar |
Ben-Dov E, Shapiro OH, Siboni N, Kushmaro A (2006) Advantage of using inosine at the 3ʹ termini of 16S rRNA gene universal primers for the study of microbial diversity. Applied and Environmental Microbiology 72, 6902–6906.
| Advantage of using inosine at the 3ʹ termini of 16S rRNA gene universal primers for the study of microbial diversity.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28Xht1Glu7bK&md5=9467f722b350a4b480ba7e5ac6b0af8aCAS |
Cai Y (1999) Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation. Journal of Dairy Science 82, 2466–2471.
| Identification and characterization of Enterococcus species isolated from forage crops and their influence on silage fermentation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXnsVGnu7w%3D&md5=42115e716b96963395ddc074d9cba2eaCAS |
Cai Y, Ohmomo S, Ogawa M, Kumai S (1997) Effect of NaCl-tolerant lactic acid bacteria and NaCl on the fermentation characteristics and aerobic stability of silage. Journal of Applied Microbiology 83, 307–313.
| Effect of NaCl-tolerant lactic acid bacteria and NaCl on the fermentation characteristics and aerobic stability of silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXms12is7c%3D&md5=846b9e65a5516862d68d19f4c0b71594CAS |
Cai Y, Benno Y, Ogawa M, Ohmomo S, Kumai S, Nakase T (1998) Influence of Lactobacillus spp. from an inoculant and of Weissella and Leuconostoc spp. from forage crops on silage fermentation. Applied and Environmental Microbiology 64, 2982–2987.
Cao Y, Cai Y, Hirakubo T, Fukui H, Matsuyama H (2011) Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons. Animal Science Journal 82, 259–266.
| Fermentation characteristics and microorganism composition of total mixed ration silage with local food by-products in different seasons.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXlt1Wns78%3D&md5=77793d37f8732cca64052104f84a2b00CAS |
Chen YS, Wu HC, Lo HY, Lin WC, Hsu WH, Lin CW, Lin PY, Yanagida F (2012) Isolation and characterisation of lactic acid bacteria from jiang-gua (fermented cucumbers), a traditional fermented food in Taiwan. Journal of the Science of Food and Agriculture 92, 2069–2075.
| Isolation and characterisation of lactic acid bacteria from jiang-gua (fermented cucumbers), a traditional fermented food in Taiwan.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XhtFSmtLg%3D&md5=c683671ed6de4863f677241e2feb7b10CAS |
Ci ZX, Gao M (2010) Tumor gastric juice ammonia nitrogen content is determined by colorimetric method improvement. Animal Husbandry and Feed Science 6, 40–41.
Cusick SM, O’Sullivan DJ (2000) Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria. Applied and Environmental Microbiology 66, 2227–2231.
| Use of a single, triplicate arbitrarily primed-PCR procedure for molecular fingerprinting of lactic acid bacteria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXjtV2ksrc%3D&md5=dede9c997946f16c5f63692ee2d8108dCAS |
Devant M, Ferret A, Gasa J, Calsamiglia S, Casals R (2000) Effects of protein concentration and degradability on performance, ruminal fermentation, and nitrogen metabolism in rapidly growing heifers fed high-concentrate diets from 100 to 230 kg body weight. Journal of Animal Science 78, 1667–1676.
| Effects of protein concentration and degradability on performance, ruminal fermentation, and nitrogen metabolism in rapidly growing heifers fed high-concentrate diets from 100 to 230 kg body weight.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXktVCktrs%3D&md5=6a685d55eda8480a1a9dd71b340b2de9CAS |
Eisen JA (1995) The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species. Journal of Molecular Evolution 41, 1105–1123.
| The RecA protein as a model molecule for molecular systematic studies of bacteria: comparison of trees of RecAs and 16S rRNAs from the same species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XivFOguw%3D%3D&md5=d48947161d0c02704308ac6dc436cbbaCAS |
Ennahar S, Cai Y, Fujita Y (2003) Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis. Applied and Environmental Microbiology 69, 444–451.
| Phylogenetic diversity of lactic acid bacteria associated with paddy rice silage as determined by 16S ribosomal DNA analysis.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXkvVaktA%3D%3D&md5=cd5be1acb5f3e12dd092e11aa8ace53bCAS |
Gollop N, Zakin V, Weinberg ZG (2005) Antibacterial activity of lactic acid bacteria included in inoculants for silage and in silages treated with these inoculants. Journal of Applied Microbiology 98, 662–666.
| Antibacterial activity of lactic acid bacteria included in inoculants for silage and in silages treated with these inoculants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXltlWisrY%3D&md5=b8264bf0d2fd281a78c58d5dad08b835CAS |
Hammes WP, Vogel RF (1995) The genus Lactobacillus. In ‘The genera of lactic acid bacteria’. pp. 19–54. (Springer: Berlin)
Khan NH, Kang TS, Grahame DA, Haakensen MC, Ratanapariyanuch K, Reaney MJ, Korber DR, Tanaka T (2013) Isolation and characterization of novel 1,3-propanediol-producing Lactobacillus panis PM1 from bioethanol thin stillage. Applied Microbiology and Biotechnology 97, 417–428.
| Isolation and characterization of novel 1,3-propanediol-producing Lactobacillus panis PM1 from bioethanol thin stillage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXjtlGnsg%3D%3D&md5=cc221805d4c41688b3b9c55238c9edc5CAS |
Kozaki M, Uchimura T, Okada S (1992) ‘Experimental manual of lactic acid bacteria.’ pp. 34–37. (Asakura Syoten: Tokyo)
Leonardi C, Giannico F, Armentano LE (2005) Effect of water addition on selective consumption (sorting) of dry diets by dairy cattle. Journal of Dairy Science 88, 1043–1049.
| Effect of water addition on selective consumption (sorting) of dry diets by dairy cattle.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXitFygtLY%3D&md5=c552ba50296031f51975a2d0183c71c2CAS |
Lin C, Bolsen KK, Brent BE, Hart RA, Dickerson JT, Feyerherm AM, Aimutis WR (1992) Epiphytic microflora on alfalfa and whole-plant corn. Journal of Dairy Science 75, 2484–2493.
| Epiphytic microflora on alfalfa and whole-plant corn.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK3s%2FosV2rtg%3D%3D&md5=6436dd3809373e185b7349fb5ec1fddaCAS |
Liu QH, Shao T, Zhang JG (2013) Determination of aerobic deterioration of corn stalk silage caused by aerobic bacteria. Animal Feed Science & Technology 183, 124–131.
Liu QH, Yang FY, Zhang JG, Shao T (2014) Characteristics of Lactobacillus parafarraginis ZH1 and its role in improving the aerobic stability of silages. Journal of Applied Microbiology 117, 405–416.
| Characteristics of Lactobacillus parafarraginis ZH1 and its role in improving the aerobic stability of silages.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhtF2lsbfF&md5=a10a922da7290d111147d5aad52f4e16CAS |
Lloyd AT, Sharp PM (1993) Evolution of the recA gene and the molecular phylogeny of bacteria. Journal of Molecular Evolution 37, 399–407.
| Evolution of the recA gene and the molecular phylogeny of bacteria.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXms1eru7Y%3D&md5=3959dd85bf75d6e42c403227db22a3acCAS |
Loučka R, Hakl J, Jirmanová J, Tyrolová Y (2015) Yearly variation in maize silage fermentation and nutritive quality. Grass and Forage Science 70, 674–681.
| Yearly variation in maize silage fermentation and nutritive quality.Crossref | GoogleScholarGoogle Scholar |
Muck RE, Pitt RE, Leibensperger RY (1991) A model of aerobic fungal growth in silage. Grass and Forage Science 46, 283–299.
Pang H, Tan Z, Qin G, Wang Y, Li Z, Jin Q, Cai Y (2012) Phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the Tibetan Plateau. Journal of Microbiology 50, 63–71.
| Phenotypic and phylogenetic analysis of lactic acid bacteria isolated from forage crops and grasses in the Tibetan Plateau.Crossref | GoogleScholarGoogle Scholar |
Ruppert LD, Drackley JK, Bremmer DR, Clark JH (2003) Effects of tallow in diets based on corn silage or alfalfa silage on digestion and nutrient use by lactating dairy cows. Journal of Dairy Science 86, 593–609.
| Effects of tallow in diets based on corn silage or alfalfa silage on digestion and nutrient use by lactating dairy cows.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXhslCltrc%3D&md5=e3575baaf8e88feafbd39ed2ba6b83eaCAS |
Russell JB, Wilson DB (1996) Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH? Journal of Dairy Science 79, 1503–1509.
| Why are ruminal cellulolytic bacteria unable to digest cellulose at low pH?Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28Xlsleltr4%3D&md5=e4f97cf7560c2e0517e8422ccdc376faCAS |
Sebastian S, Phillip L, Fellner V, Idziak E (1996) Comparative assessment of bacterial inoculated corn and sorghum silages. Journal of Animal Science 71, 505–514.
Stackebrandt E, Goebel B (1994) Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. International Journal of Systematic and Evolutionary Microbiology 44, 846–849.
| Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXitFCnsb4%3D&md5=1f8b01ddfba83c06bca71cb004d1daa2CAS |
Tan Z, Pang H, Duan Y, Qin G, Cai Y (2010) 16S ribosomal DNA analysis and characterization of lactic acid bacteria associated with traditional Tibetan Qula cheese made from yak milk. Animal Science Journal 81, 706–713.
| 16S ribosomal DNA analysis and characterization of lactic acid bacteria associated with traditional Tibetan Qula cheese made from yak milk.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjsFejtA%3D%3D&md5=87ad3bf55dc98b3460a0014803fb4763CAS |
Torriani S, Felis GE, Dellaglio F (2001) Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers. Applied and Environmental Microbiology 67, 3450–3454.
| Differentiation of Lactobacillus plantarum, L. pentosus, and L. paraplantarum by recA gene sequence analysis and multiplex PCR assay with recA gene-derived primers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXlvFSltbc%3D&md5=55f19dc384a8296cb4035d4537e4b1c3CAS |
Van Soest PJ, Robertson JB, Lewis BA (1991) Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science 74, 3583–3597.
| Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK38%2FnvVCltA%3D%3D&md5=bf240ab4dfe4d944f4f3db249ee7d298CAS |
Wilkinson J, Davies D (2013) The aerobic stability of silage: key findings and recent developments. Grass and Forage Science 68, 1–19.
| The aerobic stability of silage: key findings and recent developments.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXitVOisbc%3D&md5=ec25a8c3592dc0dd62b3e23a43a46e2dCAS |
Wu JJ, Du RP, Gao M, Sui YQ, Xiu L, Wang X (2014) Naturally occurring lactic Acid bacteria isolated from tomato pomace silage. Asian-Australasian Journal of Animal Sciences 27, 648–657.
| Naturally occurring lactic Acid bacteria isolated from tomato pomace silage.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXps1Crtb8%3D&md5=82e6786e3778d254f69377aeac619c0eCAS |
Xu C, Cai Y, Fujita Y, Kawamoto H, Sato T, Masuda N (2003) Silage preparation of barley tea grounds and their nutritive value. Animal Science Journal 74, 343–348.
