Mapping QTLs for 100-seed weight in an interspecific soybean cross of Williams 82 (Glycine max) and PI 366121 (Glycine soja)
Krishnanand P. Kulkarni A , Sovetgul Asekova A , Dong-Ho Lee A , Kristin Bilyeu B , Jong Tae Song A and Jeong-Dong Lee A C
+ Author Affiliations
- Author Affiliations
A School of Applied Biosciences, Kyungpook National University, Daegu 41566, Republic of Korea.
B USDA-ARS, Plant Genetic Research Unit, 110 Waters Hall, University of Missouri-Columbia, Columbia, MO 65211, USA.
C Corresponding author. Email: jdlee@knu.ac.kr
Crop and Pasture Science 68(2) 148-155 https://doi.org/10.1071/CP16246
Submitted: 8 July 2016 Accepted: 31 January 2017 Published: 28 February 2017
Abstract
Seed weight can be an important component for soybean quality and yield. The objective of the present study was to identify quantitative trait loci (QTLs) for 100-seed weight by using 169 recombinant inbred lines (RILs) derived from the cross Williams 82 × PI 366121. The parental lines and RILs were grown for four consecutive years (2012–15) in the field. The seeds were harvested after maturity, dried and used to measure 100-seed weight. Analysis of variance indicated significant differences among the RILs for 100-seed weight. The environment had significant effect on seed-weight expression as indicated by the genotype × environment interaction. QTL analysis employing inclusive composite interval mapping of additive QTLs implemented in QTL IciMapping (Version 4.1) identified nine QTLs (LOD >3) on chromosomes 1, 2, 6, 8, 13, 14, 17 and 20. The individual QTLs explained phenotypic variation in the range 6.1–12.4%. The QTLs were detected in one or two environments, indicating major influence of the growing environment on seed-weight expression. Four QTLs identified in this study, qSW-02_1, qSW-06_1, qSW-13_1 and qSW-14_1, were found to be new QTLs. The findings of the study may be helpful to reveal the molecular genetic basis of the seed-weight trait in soybean.
Additional keywords: QTL mapping, seed quality, wild soybean.
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