Enhancement of genetic diversity in canola-quality Brassica napus and B. juncea by interspecific hybridisation
C. J. Schelfhout A C , J. M. Wroth A B , G. Yan A and W. A. Cowling A B DA School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Canola Breeders Western Australia Pty Ltd, Locked Bag 888, Como, WA 6952, Australia.
C Current address: Department of Agriculture and Food Western Australia, PO Box 432, Merredin, WA 6415, Australia.
D Corresponding author. Email: wcowling@cyllene.uwa.edu.au
Australian Journal of Agricultural Research 59(10) 918-925 https://doi.org/10.1071/AR07425
Submitted: 13 November 2007 Accepted: 10 July 2008 Published: 18 September 2008
Abstract
Reciprocal crosses were made between Brassica napus cv. Mystic (canola) and B. juncea JN29 (near canola quality). The F1 hybrids were selfed and backcrossed in all possible combinations to parent plants. The greatest number of selfed fertile progeny were obtained when Mystic was the maternal parent, and its F1 was most successful in backcrosses to Mystic or JN29 as maternal or paternal parent. The predominant morphological type of fertile progeny was B. napus, but several B. juncea morphological types occurred in F2 and BC1-derived lines. F2 : 3 and BC1S0 : 1 progeny showed transgressive segregation for agronomic and seed quality traits in two contrasting field environments. Several of the B. juncea-type progeny had improved seed quality (lower total seed glucosinolates and higher % oleic acid) over the B. juncea parent. Selfing of interspecific hybrids between canola-quality B. napus and B. juncea has the potential to greatly enhance genetic diversity in canola-quality progeny of both species, without the loss of donor alleles that normally occurs with repeated backcrossing.
Additional keywords: oilseed rape, Indian mustard, interspecific crossing.
Acknowledgments
This research was funded by an Australian Research Council – Strategic Partnership Industry Research Training grant with co-funding provided by the Export Grains Centre Ltd and the Council of Grain Grower Organisations Ltd Near-canola quality Brassica juncea line JN29 was kindly provided for our research by Wayne Burton, Victorian Department of Primary Industries, Horsham, Victoria, Australia. Milton Sanders, Kylie Edwards, and Michael Blair at The University of Western Australia, Graham Walton and staff at the Department of Agriculture and Food Western Australia, and staff at the Merredin Research Station provided assistance with the management of field trials. Seed-quality analysis of 2001 trial seed by NIR was generously provided by Norddeutsche Pflanzenzucht Hans-Georg Lembke KG, Germany, and analysis of 2002 trial seed by NIR and GC was conducted by the Chemistry Centre of WA.
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