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RESEARCH ARTICLE
Contents Vol 59(8)

Characterisation of genetic diversity and DNA fingerprinting of Australian chickpea (Cicer arietinum L.) cultivars using MFLP markers

R. Lin A D , H. Yang B , T. N. Khan B , K. H. M. Siddique C and G. Yan C D E
+ Author Affiliations
- Author Affiliations

A Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, PR China.

B Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.

C Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

D School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

E Corresponding author. Email: gyan@plants.uwa.edu.au

Australian Journal of Agricultural Research 59(8) 707-713 https://doi.org/10.1071/AR07401
Submitted: 24 October 2007  Accepted: 21 April 2008   Published: 29 July 2008

Abstract

Chickpea (Cicer arietinum L.) is one of the major grain legume crops in the world. In this study, the genetic diversity of 24 Australian chickpea cultivars released between 1987 and 2005 was investigated with microsatellite-anchored fragment length polymorphism (MFLP) DNA markers. Among the cultivars examined, 30 cultivar-specific markers were identified and all were unequivocally identified using the DNA fingerprints developed in this study. Most of the cultivars were grouped into two major clusters; cv. Flipper was separated from the rest based on total character differences of DNA polymorphism. The MFLP approach proved suitable in the analysis of genetic diversity among the chickpea cultivars studied and the genetic relationship identified will be useful for chickpea breeding programs in selecting parent materials.

Additional keyword: cluster analysis.


Acknowledgments

This research was supported by the Centre for Legumes in Mediterranean Agriculture (CLIMA), The University of Western Australia, and the Department of Agriculture and Food Western Australia. The authors are grateful to the two anonymous reviewers for their detailed suggestions to improve the quality of this manuscript. Dr Chris Davies is thanked for the final proof-reading of this manuscript.


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