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RESEARCH ARTICLE
Contents Vol 58(8)

Yield-limiting potential of Beet western yellows virus in Brassica napus

R. A. C. Jones A B C , B. A. Coutts A and J. Hawkes A B
+ Author Affiliations
- Author Affiliations

A Agricultural Research Western Australia, Locked Bag No. 4, Bentley Delivery Centre, WA 6983, Australia.

B Centre for Legumes in Mediterranean Agriculture, University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

C Corresponding author. Email: rjones@agric.wa.gov.au

Australian Journal of Agricultural Research 58(8) 788-801 https://doi.org/10.1071/AR06391
Submitted: 11 December 2006  Accepted: 2 May 2007   Published: 30 August 2007

Abstract

Losses in seed yield and quality caused by infection with Beet western yellows virus (BWYV) alone or in combination with direct feeding damage by Myzus persicae (green peach aphid) were quantified in field experiments with Brassica napus (canola, oilseed rape) in the ‘grainbelt’ region of south-western Australia. Plants infected with BWYV and infested with M. persicae were introduced into plots early to provide infection sources and spread BWYV to B. napus plants. Insecticides were applied as seed dressings and/or foliar applications to generate a wide range of BWYV incidences in plots. Colonisation by vector aphids and spread of BWYV infection were recorded in the plots of the different treatments. At sites A (Medina) and B (Badgingarra) in 2001, foliar insecticide applications were applied differentially at first, but, later, ‘blanket’ insecticide sprays were applied to all plots to exclude any direct feeding damage by aphids. When BWYV infection at sites A and B reached 96% and 100% of plants, it decreased seed yield by up to 46% and 37%, respectively. Also, variation in BWYV incidence explained 95% (site A) and 96% (site B) of the variation in yield gaps, where for each 1% increase in virus incidence there was a yield decrease of 12 (site A) and 6 (site B) kg/ha. At both sites, this yield decline was entirely because fewer seeds formed on infected plants. At site B, BWYV infection significantly diminished oil content of seeds (up to 3%), but significantly increased individual seed weight (up to 11%) and erucic acid content (up to 44%); significant increases in seed protein content (up to 6–11%) were recorded at both sites. In field experiments at sites B and C (Avondale) in 2002, insecticides were applied as seed dressings or foliar sprays. At site B, when BWYV incidence reached 98%, the overall yield loss caused by BWYV and direct M. persicae feeding damage combined was 50%. At site C, when BWYV incidence reached 97%, the overall combined yield decline caused by BWYV and direct feeding damage was 46%. This research under Australian conditions shows that, when aphids spread it to B. napus plantings such that many plants become infected at an early growth stage, BWYV has substantial yield-limiting potential in B. napus crops. Although the results represent a worst case scenario, the losses were greater than those reported previously in Europe and are cause for concern for the Australian B. napus industry. When applied at 525 g a.i./100 kg of seed, imidacloprid seed dressing controlled insecticide-resistant M. persicae and effectively suppressed spread of BWYV for 2.5 months and increased seed yield by 84% at site B and 88% at site C. Therefore, provided that mixing the insecticide with seed is sufficiently thorough, dressing seed with imidacloprid before sowing provides good prospects for control of BWYV and M. persicae in B. napus crops.

Additional keywords: BWYV, Myzus persicae, virus, aphids, canola, oilseed rape, symptoms, insecticide, yield loss, seed quality, economic loss, control, Australia.


Acknowledgments

The authors thank Lisa Smith and Rohan Prince for technical assistance, staff at Avondale, Badgingarra, and Medina Research Stations for help with field experiments, the Western Australian Chemistry Centre for the seed erucic acid, moisture, oil, and protein analyses, Mario D’Antuono for help with statistical analysis, and Bayer Crop Science for the seed dressings. The Grains Research and Development Corporation supported this research financially.


References


Aksouh NM, Jacobs BC, Stoddard FL, Mailer RJ (2001) Response of canola to different heat stresses. Australian Journal of Agricultural Research 52, 817–824.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Berlandier F (1999) Managing aphids in canola. Agriculture Western Australia Farmnote No. 45/99.

Blackman RL , Eastop VF (2000) ‘Aphids on the world’s crops–an identification and information guide.’ (John Wiley and Sons Ltd: West Sussex, UK)

Brunt AA , Crabtree K , Dallwitz MJ , Gibbs AJ , Watson L , Zurcher EJ (Eds) (1996) ‘Viruses of plants: descriptions and lists from the VIDE database.’ (CABI: Oxford, UK)

Bwye AM, Proudlove W, Berlandier FA, Jones RAC (1997) Effects of applying insecticides to control aphid vectors and cucumber mosaic virus in narrow-leafed lupins (Lupinus angustifolius). Australian Journal of Experimental Agriculture 37, 93–102.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34, 475–483.
| PubMed |
open url image1

Coutts B , Jones R (2005) Controlling aphids and Beet western yellows virus in canola using imidacloprid seed dressing. In ‘Crop Updates Conference. 2005 Oilseeds updates’. (Ed. D Hamilton) pp. 19–20. (Department of Agriculture, Western Australia: Perth, W. Aust.)

Coutts BA, Hawkes JR, Jones RAC (2006) Occurrence of Beet western yellows virus and its aphid vectors in over-summering broad-leafed weeds and volunteer crop plants in the grainbelt region of south-western Australia. Australian Journal of Agricultural Research 57, 975–982.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Coutts BA, Jones RAC (2000) Viruses infecting canola (Brassica napus) in south-west Australia: incidence, distribution, spread and infection reservoir in wild radish (Raphanus raphanistrum). Australian Journal of Agricultural Research 51, 925–936.
| Crossref | GoogleScholarGoogle Scholar | open url image1

D’Arcy CJ , Domier LL (2005) Luteoviridae. In ‘Virus taxonomy. VIIIth Report of the International Committee on Taxonomy of Viruses’. (Eds CM Fauquet, MA Mayo, J Maniloff, U Desselberger, LA Ball) pp. 343–352. (Academic Press: New York)

Duffus JE (1972) ‘Beet western yellows virus.’ CMI/AAB Descriptions of plant viruses, No. 89. (Association of Applied Biologists: Wellesbourne, UK)

Duffus JE, Russell GE (1972) Serological relationship between beet western yellows and turnip yellows viruses. Phytopathology 62, 1274–1277. open url image1

Firestone D, Horwitz W (1979) IUPAC gas chromatographic method for determination of fatty acids composition. Collaborative study. Journal of the Association of Official Analytical Chemists 62, 709–721. open url image1

Gibbs AJ, Gower JC (1960) The use of a multiple transfer method in plant virus transmission studies—some statistical points arising in the analysis of results. Annals of Applied Biology 48, 75–83. open url image1

Graichen K (1995) Zur bedeutung von virusbefall für den anbau von winterraps und leindotter. Mitteilungen aus der Biologischen Bundesanstalt 310, 102–108. open url image1

Graichen K (1997) Wasserrubenvergilbungsvirus – ertrags- und qualistsminderungen beim winterraps. Raps 4, 156–159. open url image1

Graichen K, Rabenstein F (1996) European isolates of beet western yellows virus (BWYV) from oilseed rape (Brassica napus L. ssp. napus) are non-pathogenic on sugar beet (Beta vulgaris L. var. altissima) but represent isolates of turnip yellows virus (TuYV). Journal of Plant Diseases and Protection 103, 233–245. open url image1

Graichen K , Schliephake E (1999) Infestation of winter oilseed rape by turnip yellows luteovirus and its effect on yield in Germany. In ‘Proceedings of 10th International Rapeseed Congress—New horizons for an old crop’. (Eds N Wratten, PA Salisbury) pp. 131–136. (International Consultative Group for Rapeseed Research: Canberra, ACT)

Hauser S, Stevens M, Beuve M, Lemaire O (2002) Biological properties and molecular characterization of beet chlorosis virus (BChV). Archives of Virology 147, 745–762.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Hauser S, Stevens M, Mougel C, Smith HG, Fritsch C, Herrbach E, Lemaire O (2000) Biological, serological, and molecular variability suggest three distinct polerovirus species infecting beet or rape. Phytopathology 90, 460–466.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Hawkes J , Thackray D , Jones R (2002) Incidence of virus diseases in chickpea. In ‘Crop Updates Conference. 2002 Pulse research and industry development in Western Australia’. (Eds K Regan, P White) pp. 101–102. (Department of Agriculture, Western Australia: Perth, W. Aust.)

Jay CN, Rossall S, Smith HG (1999) Effects of beet western yellows virus on growth and yield of oilseed rape (Brassica napus). Journal of Agricultural Science, Cambridge 133, 131–139.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Jones RAC (2004) Occurrence of virus infection in seed stocks and 3-year-old pastures of lucerne (Medicago sativa). Australian Journal of Agricultural Research 55, 757–764.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Latham LJ, Jones RAC (2001) Incidence of virus infection in experimental plots, commercial crops and seed stocks of cool season crop legumes. Australian Journal of Agricultural Research 52, 397–413.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McKirdy SJ, Jones RAC (1996) Use of imidacloprid and newer generation synthetic pyrethroids to control the spread of barley yellow dwarf luteovirus in cereals. Plant Disease 80, 895–901. open url image1

McKirdy SJ, Jones RAC (1997) Effect of sowing time on barley yellow dwarf virus infection in wheat: virus incidence and grain yield losses. Australian Journal of Agricultural Research 48, 199–206.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McKirdy SJ, Jones RAC, Nutter FW (2002) Quantification of yield losses caused by Barley yellow dwarf virus in wheat and oats. Plant Disease 86, 769–773.
| Crossref | GoogleScholarGoogle Scholar | open url image1

McLean GD , Price LK (1984) Virus, viroid, mycoplasma and rickettsia diseases of plants in Western Australia. Technical Bulletin No. 68, Department of Agriculture, Perth, W. Aust.

Salisbury PA , Potter TD , McDonald G , Green AG (Eds) (1999) Canola in Australia: the first thirty years. Organising Committee of the 10th International Rapeseed Congress, Canberra, ACT

Schroder M (1994) Investigations on the susceptibility of oilseed rape (Brassica napus L. spp. napus) to different virus diseases. Zeitschrift für Pflanzenkrankheiten und Pflanzenschutz 101, 576–589. open url image1

Smith HG , Barker H (Eds) (1999) ‘The Luteoviridae.’ (CABI Publishing: Wallingford, UK)

Smith HG, Hinckes JA (1985) Studies on beet western yellows virus in oilseed rape (Brassica napus ssp. oleifera) and sugar beet (Beta vulgaris). Annals of Applied Biology 107, 473–484. open url image1

Thackray D , Hawkes J , Jones R (2000 b) Forecasting aphid and virus risk in canola. In ‘Crop Updates Conference. 2000 Oilseeds updates’. (Ed. A Cox) pp. 36–37. (Agriculture Western Australia: Perth, W. Aust.)

Thackray D , Hawkes J , Jones R (2001) Further developments in forecasting aphid and virus risk in canola. In ‘Crop Updates Conference. 2001 Oilseeds updates’. (Ed. C Zaicou-Kunesch) pp. 55–57. (Agriculture Western Australia: Perth, W. Aust.)

Thackray D , Hawkes J , Jones R (2002) Influence of climate on aphid outbreaks and virus epidemics in canola. In ‘Crop Updates Conference. 2002 Oilseeds updates’. (Ed. D Ecksteen) pp. 44–46. (Department of Agriculture, Western Australia: Perth, W. Aust.)

Thackray DJ, Diggle AJ, Berlandier FA, Jones RAC (2004) Forecasting aphid outbreaks and epidemics of Cucumber mosaic virus in lupin crops in a Mediterranean-type environment. Virus Research 100, 67–82.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Thackray DJ, Jones RAC (2003) Forecasting aphid outbreaks and epidemics of Barley yellow dwarf virus – a decision support system for a Mediterranean-type climate. Australasian Plant Pathology [Abstr.] 32, 438. open url image1

Thackray DJ, Jones RAC, Bwye AM, Coutts BA (2000a) Further studies on the effects of insecticides on aphid vector numbers and spread of cucumber mosaic virus in narrow-leafed lupins (Lupinus angustifolius). Crop Protection 19, 121–139.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Thackray DJ, Ward LT, Thomas-Carroll ML, Jones RAC (2005) Role of winter-active aphids spreading Barley yellow dwarf virus in decreasing wheat yields in a Mediterranean-type environment. Australian Journal of Agricultural Research 56, 1089–1099.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Walsh JA, Perrin RM, Miller A, Laycock DS (1989) Studies on beet western yellows virus in winter oilseed rape (Brassica napus spp. oleifera) and the effect of the insecticidal treatment on its spread. Crop Protection 8, 137–143.
| Crossref |
open url image1

Walsh JA, Tomlinson JA (1985) Viruses infecting winter oilseed rape (Brassica napus ssp. oleifera). Annals of Applied Biology 107, 485–495. open url image1