Simple sequence repeat markers associated with three quantitative trait loci for black point resistance can be used to enrich selection populations in bread wheat
M. J. Christopher A C , P. M. Williamson A , M. Michalowitz A , R. Jennings A , A. Lehmensiek B , J. Sheppard A and P. Banks AA Leslie Research Centre, Queensland Department of Primary Industries and Fisheries, 13 Holberton Street, Toowoomba, Qld 4350, Australia.
B University of Southern Queensland, Toowoomba, Qld 4350, Australia.
C Corresponding author. Email: mandy.christopher@dpi.qld.gov.au
Australian Journal of Agricultural Research 58(9) 867-873 https://doi.org/10.1071/AR05435
Submitted: 12 December 2005 Accepted: 25 June 2007 Published: 28 September 2007
Abstract
Black point in wheat has the potential to cost the Australian industry $A30.4 million a year. It is difficult and expensive to screen for resistance, so the aim of this study was to validate 3 previously identified quantitative trait loci (QTLs) for black point resistance on chromosomes 2B, 4A, and 3D of the wheat variety Sunco. Black point resistance data and simple sequence repeat (SSR) markers, linked to the resistance QTLs and suited to high-throughput assay, were analysed in the doubled haploid population, Batavia (susceptible) × Pelsart (resistant). Sunco and Pelsart both have Cook in their pedigree and both have the Triticum timopheevii translocation on 2B. SSR markers identified for the 3 genetic regions were gwm319 (2B, T. timopheevii translocation), wmc048 (4AS), and gwm341 (3DS). Gwm319 and wmc048 were associated with black point resistance in the validation population. Gwm341 may have an epistatic influence on the trait because when resistance alleles were present at both gwm319 and wmc048, the Batavia-derived allele at gwm341 was associated with a higher proportion of resistant lines. Data are presented showing the level of enrichment achieved for black point resistance, using 1, 2, or 3 of these molecular markers, and the number of associated discarded resistant lines. The level of population enrichment was found to be 1.83-fold with 6 of 17 resistant lines discarded when gwm319 and wmc048 were both used for selection. Interactions among the 3 QTLs appear complex and other genetic and epigenetic factors influence susceptibility to black point. Polymorphism was assessed for these markers within potential breeding material. This indicated that alternative markers to wmc048 may be required for some parental combinations. Based on these results, marker-assisted selection for the major black point resistance QTLs can increase the rate of genetic gain by improving the selection efficiency and may facilitate stacking of black point resistances from different sources.
Additional keywords: Triticum aestivum, SSR markers, validation.
Acknowledgments
We thank the Grains Research and Development Corporation for funding the work through the Australian Winter Cereals Molecular Marker Program, and Dr Steve Kammholz for access to the Batavia × Pelsart DH population.
Allard RW, Shands R
(1954) Inheritance of resistance to stem rust and powdery mildew in cytologically stable spring wheats derived from Triticum timopheevii. Phytopathology 44, 266–274.
(Accessed on: 21/06/2004).
Bassam BJ, Caetano-Annollés G
(1993) Silver staining of DNA in polyacrylamide gels. Applied Biochemistry and Biotechnology 42, 181–188.
(Accessed on: 23/05/2003).
Friebe B,
Jiang J,
Raupp WJ,
McIntosh RA, Gill BS
(1996) Characterization of wheat-alien translocations conferring resistance to disease and pests: current status. Euphytica 91, 59–87.
| Crossref | GoogleScholarGoogle Scholar |
Garland S,
Lewin L,
Blakeney A, Reinke R
(2000) PCR based molecular markers for the fragrance gene in rice (Oryza sativa L.). Theoretical and Applied Genetics 101, 364–371.
| Crossref | GoogleScholarGoogle Scholar |
Gupta PK,
Varshney RK,
Sharma PC, Ramesh B
(1999) Molecular markers and their applications in wheat breeding. Plant Breeding 118, 369–390.
| Crossref | GoogleScholarGoogle Scholar |
Harjit-Singh
,
Prasad M,
Varshney RK,
Roy JK,
Balyan HS,
Shaliwas HS, Gupta PK
(2001) STMS markers for grain protein content and their validation using near-isogenic lines in bread wheat. Plant Breeding 120, 273–278.
| Crossref | GoogleScholarGoogle Scholar |
Jefferies SP,
Pallotta MA,
Paull JG,
Karakousis A,
Kretschmer JM,
Manning S,
Islam AKMR,
Langridge P, Chalmers KJ
(2000) Mapping and validation of chromosome regions conferring boron toxicity tolerance in wheat (Triticum aestivum). Theoretical and Applied Genetics 101, 767–777.
| Crossref | GoogleScholarGoogle Scholar |
Langridge P,
Lagudah ES,
Holton TA,
Appels R,
Sharp PJ, Chalmers KJ
(2001) Trends in genetic and genome analyses in wheat: a review. Australian Journal of Agricultural Research 52, 1043–1077.
| Crossref | GoogleScholarGoogle Scholar |
Laurie DA, Bennett MD
(1986) Wheat × maize hybridisation. Canadian Journal of Genetics and Cytology 28, 313–316.
Lehmensiek A,
Campbell AW,
Williamson PM,
Michalowitz M,
Sutherland MW, Daggard GE
(2004) QTLs for black point resistance in wheat and the identification of potential markers for use in breeding programs. Plant Breeding 123, 410–416.
| Crossref | GoogleScholarGoogle Scholar |
Machacek JE, Greaney FJ
(1938) The black-point or kernel smudge disease of cereals. Canadian Journal of Research 16, 84–113.
Manly KF,
Cudmore RH, Meer JM
(2001) Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12, 930–932.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Nyquist NQ
(1962) Differential fertilization in the inheritance of stem rust resistance in hybrids involving a common wheat strain derived from Triticum timopheevii. Genetics 47, 1109–1124.
| PubMed |
Prasad M,
Kumar N,
Kulwal PL,
Röder MS,
Balyan HS,
Dhaliwal HS, Gupta PK
(2003) QTL analysis for grain protein content using SSR markers and validation studies using NILs in bread wheat. Theoretical and Applied Genetics 106, 659–667.
| PubMed |
QDPI
(1994) Variety: ‘Pelsart’. Application no. 93/187. Plant Varieties Journal 7, 23.
Röder MS,
Korzun V,
Wendehake K,
Plaschke J,
Tixier M-H,
Leroy P, Ganal MW
(1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |
Schnurbusch T,
Bossolini E,
Messmer M, Keller B
(2004) Tagging and validation of a major quantitative trait locus for leaf rust resistance and leaf tip necrosis in winter wheat cultivar Forno. Phytopathology 94, 1036–1041.
| Crossref | GoogleScholarGoogle Scholar |
Sears ER
(1993) Use of radiation to transfer alien chromosome segments to wheat. Crop Science 33, 897–901.
Sharp J,
Johnston S,
Brown G,
McIntosh RA, Pallotta M , et al.
(2001) Validation of molecular markers for wheat breeding. Australian Journal of Agricultural Research 52, 1357–1366.
| Crossref | GoogleScholarGoogle Scholar |
Song W, Henry RJ
(1995) Molecular analysis of the DNA polymorphism of wild barley (Hordeum spontaneum) germplasm using the polymerase chain reaction. Genetic Resources and Crop Evolution 42, 273–281.
| Crossref | GoogleScholarGoogle Scholar |
Torada A,
Ikeguchi S, Koike M
(2005) Mapping and validation of PCR-based markers associated with a major QTL for seed dormancy in wheat. Euphytica 143, 251–255.
| Crossref | GoogleScholarGoogle Scholar |
Williamson PM
(1997) Black point in wheat: in vitro production of symptoms, enzymes involved, and association with Alternaria alternata. Australian Journal of Agricultural Research 48, 13–19.
| Crossref | GoogleScholarGoogle Scholar |
Zhou W-C,
Kolb FL,
Bai G-H,
Domier LL,
Boze LK, Smith NJ
(2003) Validation of a major QTL for scab resistance with SSR markers and use of marker-assisted selection in wheat. Plant Breeding 122, 40–46.
| Crossref | GoogleScholarGoogle Scholar |
