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RESEARCH ARTICLE
Contents Vol 57(5)

Mapping quantitative trait loci for resistance to Pratylenchus thornei from synthetic hexaploid wheat in the International Triticeae Mapping Initiative (ITMI) population

R. S. Zwart A , J. P. Thompson A C , J. G. Sheedy A and J. C. Nelson B
+ Author Affiliations
- Author Affiliations

A Department of Primary Industries and Fisheries, PO Box 2282, Toowoomba, Qld 4350, Australia.

B Department of Plant Pathology, 4024 Throckmorton Hall, Kansas State University, Manhattan, KS, USA.

C Corresponding author. Email: john.thompson@dpi.qld.gov.au

Australian Journal of Agricultural Research 57(5) 525-530 https://doi.org/10.1071/AR05177
Submitted: 18 May 2005  Accepted: 7 December 2005   Published: 17 May 2006

Abstract

Root-lesion nematode (Pratylenchus thornei) is a serious pathogen of wheat in many countries. The International Triticeae Mapping Initiative (ITMI) population of recombinant inbred lines (RILs) was assessed for resistance to P. thornei to determine the chromosome locations of the resistance genes. The ITMI population is derived from a cross between the resistant synthetic hexaploid wheat W-7984 and a susceptible bread wheat cultivar Opata 85. Two years of phenotypic data for resistance to P. thornei were obtained in replicated glasshouse trials. Quantitative trait locus (QTL) analysis was performed using available segregation and map data for 114 RILs. A QTL on chromosome 6DS showed consistent effects for reduced nematode numbers (partial resistance) across years and accounted for 11% and 23% of the phenotypic variation. A second QTL for P. thornei resistance on chromosome 2BS accounted for an additional 19% and 5%. Restriction fragment length polymorphism (RFLP) and simple sequence repeat (SSR) markers associated with the QTLs are physically located in regions rich in major genes at the distal ends of the short chromosome arms of 6D and 2B. SSR markers with potential for marker-assisted selection of P. thornei resistance effective in different genetic backgrounds have been identified.

Additional keywords: root-lesion nematodes, bread wheat, molecular markers, MAS, composite interval mapping, QTL Cartographer, W-7984, Opata 85.


Acknowledgments

We wish to thank T. G. Clewett for technical assistance, Dr P. J. Sharp, University of Sydney, Australia, and Dr K. J. Chalmers, University of Adelaide, Australia, for providing seed of the ITMI population, and Dr P. S. Brennan for suggesting an investigation of P. thornei resistance in this population. This work was supported by the Grains Research and Development Corporation of Australia.


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