Quantitative trait loci for root lesion nematode (Pratylenchus thornei) resistance in Middle-Eastern landraces and their potential for introgression into Australian bread wheat
A. L. Schmidt A C , C. L. McIntyre A , J. Thompson B , N. P. Seymour B and C. J. Liu AA CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Qld 4067, Australia.
B Queensland Department of Primary Industries and Fisheries, Leslie Research Centre, PO Box 2282, Toowoomba, Qld 4350, Australia.
C Corresponding author. Email: Adele.Schmidt@csiro.au
Australian Journal of Agricultural Research 56(10) 1059-1068 https://doi.org/10.1071/AR05016
Submitted: 17 January 2005 Accepted: 30 June 2005 Published: 25 October 2005
Abstract
Plant parasitic nematodes are a major biotic cause of wheat yield loss in temperate wheat-growing regions. Previous studies using Australian germplasm and/or synthetic hexaploid lines have identified quantitative trait loci (QTLs) for root lesion nematode resistance on chromosomes 2B, 6D, and 7A. This study examines Pratylenchus thornei resistance in 2 Middle-Eastern landraces (AUS13124 and AUS4926), using doubled haploid populations generated by crossing with the susceptible Australian cultivar Janz. Single marker regression and QTL analysis identified resistance loci on chromosomes 2B, 3B, 6D, and 7A, and a susceptibility locus on chromosome 1B. The 2B and 6D loci, which have been reported to explain up to 19% and 24% of variation, respectively, in previous studies, made smaller contributions in the Middle-Eastern varieties, explaining 2–13% (2B) and 1–6% (6D) of phenotypic variation in these populations. The previously reported 7A locus (P. neglectus resistance) was detected through single marker regression only (AUS13124 × Janz – LRS = 4.1, P = 0.04292; AUS4926 × Janz – LRS = 9.6, P = 0.00195), with genotype at the microsatellite marker Xgwm350.3 accounting for 3–23% of phenotypic variation. The previously unreported resistance QTL, located on chromosome 3B, explained up to 24% of phenotypic variation, and the susceptibility locus on chromosome 1B explained up to 21%. The 3B locus was detected in both the AUS13124 × Janz (max. LRS = 20.13) and AUS4926 × Janz (max. LRS = 11.19) populations, and the 1B locus was detected in the AUS4926 × Janz population (max. LRS = 18.82) only.
Additional keyword: QTL.
Acknowledgments
This work was funded by a research grant from the Australian Grains Research and Development Corporation, and was conducted as part of the Australian Winter Cereals Molecular Marker Program. The results reported in this study were generated as part of a collaborative research program designed to address issues specific to Australia’s northern wheat-growing region, involving staff from CSIRO Plant Industry, The University of Southern Queensland, CIMMYT, and Queensland Department of Primary Industries. Within this program, R. Grams and R. Zwart, and M. Sutherland made contributions to this work. M. Miyagi assisted with phenotyping.
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