Development of DNA fingerprinting keys for discrimination of Cicer echinospermum (P.H. Davis) accessions using AFLP markers
Fucheng Shan A C , Heather Clarke A , Guijun Yan B , Julie A. Plummer B and Kadambot H. M. Siddique AA Centre for Legumes in Mediterranean Agriculture, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.
B School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.
C Corresponding author; email: fshan@agric.uwa.edu.au
Australian Journal of Agricultural Research 55(9) 947-952 https://doi.org/10.1071/AR04080
Submitted: 8 April 2004 Accepted: 16 July 2004 Published: 24 September 2004
Abstract
To test the hypothesis that DNA markers associated with specific genetic make-up can be detected and used to discriminate genotypes, amplified fragment length polymorphism (AFLP) markers were produced for 14 accessions in Cicer echinospermum, a close relative of Cicer arietinum (chickpea). Six selective amplification primer combinations produced high polymorphism with average polymorphic loci of 77.2%. The polymorphism detected in this study enabled fingerprinting keys to be established to discriminate accessions within C. echinospermum. Results showed that molecular analysis using AFLP was a good and reliable technique to differentiate C. echinospermum accessions and to reconstruct phylogenetic relationships between them, which could help parental selection in chickpea improvement programs.
Additional keywords: genetic diversity, chickpea, legumes.
Acknowledgments
Fucheng Shan is grateful to the Grains Research and Development Corporation of Australia (GRDC) for a Postdoctoral Fellowship (PDF38). We thank Mr Ted Knight, Dr Jens Berger, ICARDA, and USDA for providing germplasm. The molecular analysis in this research was conducted using facilities at the State Agricultural Biotechnology Centre, Murdoch University, WA.
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