Variation in a worldwide collection of fennel (Foeniculum vulgare var. vulgare)
Elaheh Hosseini A , Mohammad Mahdi Majidi
A * , Mohammad Hossein Ehtemam A and Mustafa Ghanadian B
+ Author Affiliations
- Author Affiliations
A Department of Agronomy and Plant Breeding, College of Agriculture, Isfahan University of Technology, Isfahan 84156-83111, Iran.
B Department of Pharmacognosy, Isfahan Pharmaceutical Sciences Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Crop & Pasture Science 72(12) 1008-1021 https://doi.org/10.1071/CP21149
Submitted: 2 March 2021 Accepted: 28 July 2021 Published: 7 December 2021
© 2021 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Fennel (Foeniculum vulgare Mill.) is an old medicinal plant and has been commonly used as a traditional food and medicine. This study aimed to evaluate genetic variation for agro-morphological traits and essential oil content and components in a worldwide collection of bitter fennel (F. vulgare var. vulgare). We examined production stability of different accessions, and identified associations between traits in order to find the best criteria for use in future breeding programs. The study evaluated 33 bitter fennel accessions from 11 countries in the field for morphological characters across 4 years (2015–18), and for important phytochemical characters in 2 years (2015 and 2016). Comparisons across years revealed varied responses from fennel genotypes. Highest mean production was observed in the third year, and highest essential oil content in the second year. Based on gas chromatography–mass spectrometry analysis, trans-anethole (59.86–78.82% of total oil content), estragole (3.61–24.39%), fenchone (9.58–18.26%) and limonene (0.52–3.66%) were detected as major essential oil components in the studied germplasm. Based on principal component and cluster analysis, genotypes were grouped into different clusters. Classification of genotypes was mainly in agreement with available information regarding their origins. The results support available information on the history of human establishments and the introduction of fennel from Mediterranean gene pools to other regions in ancient times. The negative correlation between some essential oil components indicates that some components are isomers and this could limit the simultaneous selection of components. Superior genotypes were identified in the studied germplasm, which can be used for future breeding programs with specific purposes.
Key words: essential oil, exotic germplasm, fennel, GC–MS, germplasm, medicinal plant, production stability, selection, variation.
References
Abou El-Nasr THS, Ibrahim MM, Aboud KA, Al-Kordy MAA (2013) Genetic variation among three fennels (Foeniculum vulgare Mill.) varieties on the basis of morphological characters, essential oil composition and ISSR markers. Journal of Applied Sciences Research 9, 1594–1603.Adams RP (2004) ‘Identification of essential oil components by gas chromatography/quadrupole mass spectrometry’, (Allured Publishing Corporation: Carol Stream, IL, USA)
Anwar F, Hussain AI, Sherazi STH, Bhanger MI (2009) Changes in composition and antioxidant and antimicrobial activities of essential oil of fennel (Foeniculum vulgare Mill.) fruit at different stages of maturity. Journal of Herbs, Spices & Medicinal Plants 15, 187–202.
| Changes in composition and antioxidant and antimicrobial activities of essential oil of fennel (Foeniculum vulgare Mill.) fruit at different stages of maturity.Crossref | GoogleScholarGoogle Scholar |
Aprotosoaie AC, Şpac A, Hăncianu M, Miron A, Tănăsescu VF, Dorneanu V, Stănescu U (2010) The chemical profile of essential oils obtained from fennel fruits (Foeniculum vulgare Mill.). Farmacia 58, 46–53.
Bahmani K, Izadi-Darbandi A, Sadat Noori SA, Ashraf Jafari A, Moradi N (2012) Determination of interrelationships among phenotypic traits of Iranian fennel (Foeniculum vulgare Mill) using correlation, stepwise regression and path analyses. Journal of Essential Oil Bearing Plants 15, 424–444.
| Determination of interrelationships among phenotypic traits of Iranian fennel (Foeniculum vulgare Mill) using correlation, stepwise regression and path analyses.Crossref | GoogleScholarGoogle Scholar |
Bailer J, Aichinger T, Hackl G, de Hueber K, Dachler M (2001) Essential oil content and composition in commercially available dill cultivars in comparison to caraway. Industrial Crops and Products 14, 229–239.
| Essential oil content and composition in commercially available dill cultivars in comparison to caraway.Crossref | GoogleScholarGoogle Scholar |
Bernáth J, Németh É, Kattaa A, Hétheli É (1996) Morphological and chemical evaluation of fennel (Foeniculum vulgare Mill.) populations of different origin. Journal of Essential Oil Research 8, 247–253.
| Morphological and chemical evaluation of fennel (Foeniculum vulgare Mill.) populations of different origin.Crossref | GoogleScholarGoogle Scholar |
Bettaieb Rebey I, Jabri-Karoui I, Hamrouni-Sellami I, Bourgou S, Limam F, Marzouk B (2012) Effect of drought on the biochemical composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds. Industrial Crops and Products 36, 238–245.
| Effect of drought on the biochemical composition and antioxidant activities of cumin (Cuminum cyminum L.) seeds.Crossref | GoogleScholarGoogle Scholar |
Bilia AR, Flamini G, Taglioli V, Morelli I, Vincieri FF (2002) GC–MS analysis of essential oil of some commercial fennel teas. Food Chemistry 76, 307–310.
| GC–MS analysis of essential oil of some commercial fennel teas.Crossref | GoogleScholarGoogle Scholar |
Bowes KM, Zheljazkov VD (2005) Essential oil yield and quality of fennel growth in Nova Scotia. HortScience 39, 1640–1643.
| Essential oil yield and quality of fennel growth in Nova Scotia.Crossref | GoogleScholarGoogle Scholar |
British Pharmacopoeia Commission (1988) ‘British Pharmacopoeia. Vol. 2’, (HMSO: London, UK)
Burton GW, DeVane EH (1953) Estimation of heritability in tall Festuca (Festuca arundinacea) from replicated clonally material. Agronomy Journal 45, 478–481.
| Estimation of heritability in tall Festuca (Festuca arundinacea) from replicated clonally material.Crossref | GoogleScholarGoogle Scholar |
Charles DJ, Morales MR, Simon JE (1993) Essential oil content and chemical composition of finocchio fennel. In ‘New crops’. (Eds J Janick, JE Simon) pp. 570–573. (Wiley: Hoboken, NJ, USA)
Choudhary AR, Shah AH, Ali L, Bashir M (1986) Path coefficient analysis of yield and yield components in wheat. Pakistan Journal of Agricultural Research 7, 71–75.
| Path coefficient analysis of yield and yield components in wheat.Crossref | GoogleScholarGoogle Scholar |
Choudhary S, Sharma R, Meena RS, Verma AK (2018) Molecular diversity analysis in fennel (Foeniculum vulgare Mill) genotypes and its implications for conservation and crop breeding. International Journal of Current Microbiology and Applied Sciences 7, 794–809.
| Molecular diversity analysis in fennel (Foeniculum vulgare Mill) genotypes and its implications for conservation and crop breeding.Crossref | GoogleScholarGoogle Scholar |
Coşge B, Kiralan M, Gürbüz B (2008) Characteristics of fatty acids and essential oil from sweet fennel (Foeniculum vulgare Mill. var. dulce) and bitter fennel fruits (F. vulgare Mill. var. vulgare) growing in Turkey. Natural Product Research 22, 1011–1016.
| Characteristics of fatty acids and essential oil from sweet fennel (Foeniculum vulgare Mill. var. dulce) and bitter fennel fruits (F. vulgare Mill. var. vulgare) growing in Turkey.Crossref | GoogleScholarGoogle Scholar | 18780240PubMed |
Dashora A, Sastry EVD (2011) Variability, character association and path coefficient analysis in fennel. Indian Journal of Horticulture 68, 351–356.
De Vincenzi M, Silano M, Maialetti F, Scazzocchio B (2000) Constituents of aromatic plants: II. Estragole. Fitoterapia 71, 725–729.
| Constituents of aromatic plants: II. Estragole.Crossref | GoogleScholarGoogle Scholar | 11077188PubMed |
Dobos G, Novak J (2005) Comparison of the composition of the essential oil of some winter-annually cultivated coriander accessions (Coriandrum sativum L.). Zeitschrift fur Arznei- & Gewurzpflanzen 10, 144–145.
Espanani S, Majidi MM, Saeidi G, Alaei H, Rezaei V (2019) Wide hybridization and introgression breeding in safflower: Effectiveness of different selection methods. Plant Breeding 138, 846–861.
| Wide hybridization and introgression breeding in safflower: Effectiveness of different selection methods.Crossref | GoogleScholarGoogle Scholar |
Fufa H, Baenziger PS, Beecher BS, Dweikat I, Graybosch RA, Eskridge KM (2005) Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars. Euphytica 145, 133–146.
| Comparison of phenotypic and molecular marker-based classifications of hard red winter wheat cultivars.Crossref | GoogleScholarGoogle Scholar |
Gross M, Lewinsohn E, Tadmor Y, Bar E, Dudai N, Cohen Y, Friedma J (2009) The inheritance of volatile phenylpropenes in bitter fennel (Foeniculum vulgare Mill. var. vulgare Apiaceae) chemotypes and their distribution within the plant. Biochemical Systematics and Ecology 37, 308–316.
| The inheritance of volatile phenylpropenes in bitter fennel (Foeniculum vulgare Mill. var. vulgare Apiaceae) chemotypes and their distribution within the plant.Crossref | GoogleScholarGoogle Scholar |
Kahrizi D, Azizi K, Haghi Y, Zebarjadi A (2011) Relationships among yield and yield components and essence in cumin (Cuminum cyminum L.) under different climatic conditions. Planta Medica 77, PE26
| Relationships among yield and yield components and essence in cumin (Cuminum cyminum L.) under different climatic conditions.Crossref | GoogleScholarGoogle Scholar |
Kalleli F, Rebey IB, Razgallah N, Saidani Tounsi M, Hamidi MM (2019) Comparative analysis of morphological and chemical variability in different fennel (Foeniculum vulgare Mill.) cultivars. International Journal of Agriculture, Environment and Bioresearch 4, 200–217.
| Comparative analysis of morphological and chemical variability in different fennel (Foeniculum vulgare Mill.) cultivars.Crossref | GoogleScholarGoogle Scholar |
Kingsley-Richards SL (2011) Influence of plant age, soil moisture, and temperature cycling date on container-grown herbaceous perennials. MS Thesis, University of Vermont, Burlington, VT, USA.
Kumar R, Meena RS, Verma AK, Hemant A, Panwar A (2017) Analysis of genetic variability and correlation in fennel (Foeniculum vulgare Mill.) germplasm. Agricultural Research and Technology 3, 125–129.
| Analysis of genetic variability and correlation in fennel (Foeniculum vulgare Mill.) germplasm.Crossref | GoogleScholarGoogle Scholar |
Lal RK (2008) Stability and genotypes × environment interactions in fennel. Journal of Herbs, Spices & Medicinal Plants 13, 47–54.
| Stability and genotypes × environment interactions in fennel.Crossref | GoogleScholarGoogle Scholar |
Lawrence BM (1994) Progress in essential oils. Perfumer and Flavorist 19, 31–32.
Lopes VR, Barata AM, Farias RM, Mendes MD, Lima AS, Pedro LG, Barroso JG, Figueiredo AC (2010) Morphological and essential oil variability from nine Portuguese fennel (Foeniculum vulgare Mill.) accessions. Acta Horticulturae 860, 33–49.
| Morphological and essential oil variability from nine Portuguese fennel (Foeniculum vulgare Mill.) accessions.Crossref | GoogleScholarGoogle Scholar |
López PA, Widrlechner MP, Simon PW, Rai S, Boylston TD, Isbell TA, Bailey TB, Gardner CA, Wilson LA (2008) Assessing phenotypic, biochemical and molecular diversity in coriander (Coriandrum sativum L.) germplasm. Genetic Resources and Crop Evolution 55, 247–275.
| Assessing phenotypic, biochemical and molecular diversity in coriander (Coriandrum sativum L.) germplasm.Crossref | GoogleScholarGoogle Scholar |
Majidi MM, Mirlohi A, Amini F (2009) Genetic variation, heritability and correlations of agro-morphological traits in tall fescue (Festuca arundinacea Schreb.). Euphytica 167, 323–331.
| Genetic variation, heritability and correlations of agro-morphological traits in tall fescue (Festuca arundinacea Schreb.).Crossref | GoogleScholarGoogle Scholar |
Majidi MM, Zadhoush S (2014) Molecular and morphological variation in a world-wide collection of safflower. Crop Science 54, 2109–2119.
| Molecular and morphological variation in a world-wide collection of safflower.Crossref | GoogleScholarGoogle Scholar |
Malhotra SK (2012) Fennel and fennel seed. In ‘Handbook of herbs and spices’. 2nd edn. (Ed KV Peter) pp. 275–302. (Wood Head Publishing: Cambridge, UK)
Meena RS, Kakani RK, Anwer MM, Panwar A (2010) Variability of some morphological characters in fennel (Foeniculum vulgare Mill.). Indian Journal of Agricultural Sciences 80, 710–712.
Meena RS, Lal G, Meena NK, Mali SC (2019a) Variability and character association in fennel (Foeniculum vulgare Mill.) germplasm. International Journal of Seed Spices 9, 29–36.
Meena RS, Verma AK, Meena NK, Ahari A, Balai SR, Mali SC (2019b) Variability and correlation studies for yield and yield contributing traits in fennel (Foeniculum vulgare Mill.) germplasm. International Journal of Seed Spices 9, 72
Moser BR, Zheljazkov VD, Bakota EL, Evangelista RL, Gawde A, Cantrell CL, Winkler-Moser JK, Hristov AN, Astatkie T, Jeliazkova E (2014) Method for obtaining three products with different properties from fennel (Foeniculum vulgare) seed. Industrial Crops and Products 60, 335–342.
| Method for obtaining three products with different properties from fennel (Foeniculum vulgare) seed.Crossref | GoogleScholarGoogle Scholar |
Oliveira PF, Alves JM, Damasceno JL, Oliveira RAM, Dias HJ, Crotti AEM, Tavares DC (2015) Cytotoxicity screening of essential oils in cancer cell lines. Revista Brasileira de Farmacognosia 25, 183–188.
| Cytotoxicity screening of essential oils in cancer cell lines.Crossref | GoogleScholarGoogle Scholar |
Olle M, Bender I (2010) The content of oils in umbelliferous crops and its formation. Agronomy Research 8, 687–696.
Pasrija A, Singh R, Katiyar CK (2011) Standardization of fennel (Foeniculum vulgare), its oleoresin and marketed ayurvedic dosage forms. International Journal of Pharmaceutical Sciences and Drug Research 3, 265–269.
Patel DG, Patel PS, Patel ID (2008) Studies on variability of some morphological characters in fennel Foeniculum vulgare types of wild fennel (Foeniculum vulgare Mill.). Journal of Agricultural and Food Chemistry 49, 239–244.
Patel RP, Singh R, Rajeswara Rao BR, Singh RR, Srivastavae A, Lal RK (2016) Differential response of genotype × environment on phenology, essential oil yield and quality of natural aroma chemicals of five Ocimum species. Industrial Crops and Products 87, 210–217.
| Differential response of genotype × environment on phenology, essential oil yield and quality of natural aroma chemicals of five Ocimum species.Crossref | GoogleScholarGoogle Scholar |
Piccaglia R, Marotti M (2001) Characterization of some Italian types of wild fennel (Foeniculum vulgare Mill.). Journal of Agricultural and Food Chemistry 49, 239–244.
| Characterization of some Italian types of wild fennel (Foeniculum vulgare Mill.).Crossref | GoogleScholarGoogle Scholar | 11170583PubMed |
Poudineh Z, Fakheri BA, Sirosmehr AR, Shojaei S (2018) Effect of drought stress on the morphology and antioxidant enzymes activity of Foeniculum vulgare cultivars in Sistan. Indian Journal of Plant Physiology 23, 283–292.
| Effect of drought stress on the morphology and antioxidant enzymes activity of Foeniculum vulgare cultivars in Sistan.Crossref | GoogleScholarGoogle Scholar |
Raal A, Orav A, Arak E (2012) Essential oil composition of Foeniculum vulgare Mill. fruits from pharmacies in different countries. Natural Product Research 26, 1173–1178.
| Essential oil composition of Foeniculum vulgare Mill. fruits from pharmacies in different countries.Crossref | GoogleScholarGoogle Scholar | 21827282PubMed |
Raghavan S (2006) ‘Handbook of spices, seasoning and flavoring’, 2nd edn. (CRC Press Taylor and Francis Group: Boca Raton, FL, USA)
Rather MA, Dar BA, Sofi SN, Bhat BA, Qurishi MA (2016) Foeniculum vulgare: a comprehensive review of its traditional use, phytochemistry, pharmacology, and safety. Arabian Journal of Chemistry 9, S1574–S1583.
| Foeniculum vulgare: a comprehensive review of its traditional use, phytochemistry, pharmacology, and safety.Crossref | GoogleScholarGoogle Scholar |
Roldán-Ruiz R, Dendauw J, Van Bockstaele E, Depicker A, De Loose M (2000) AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.). Molecular Breeding 6, 125–134.
| AFLP markers reveal high polymorphic rates in ryegrasses (Lolium spp.).Crossref | GoogleScholarGoogle Scholar |
Saini N, Singh GK, Nagori BP (2014) Physicochemical characterization and spasmolytic activity of essential oil of fennel (Foeniculum vulgare Linn.) from Rajasthan. International Journal of Pharmaceutics 4, 97–103.
Salami HA, Adjanohoun A, Padonou W, Yacoubou A-M, Aly D, Yallou C, Sina H, Baba-Moussa L (2015) Morphological diversity of corn’s (Zea mays L.) local cultivar and improved varieties in central and north of Benin. American Journal of Plant Sciences 6, 2867–2877.
| Morphological diversity of corn’s (Zea mays L.) local cultivar and improved varieties in central and north of Benin.Crossref | GoogleScholarGoogle Scholar |
Seidler-Lozykowska K, Baranska M, Baranski R, Krol D (2010) Raman analysis of caraway (Carum carvi L.) single fruits. Evaluation of essential oil content and its composition. Journal of Agricultural and Food Chemistry 58, 5271–5275.
| Raman analysis of caraway (Carum carvi L.) single fruits. Evaluation of essential oil content and its composition.Crossref | GoogleScholarGoogle Scholar | 20402506PubMed |
Shavrukov Y, Kurishbayev A, Jatayev S, Shvidchenko V, Zotova L, Koekemoer F, de Groot S, Soole K, Langridge P (2017) Early flowering as a drought escape mechanism in plants: how can it aid wheat production? Frontiers in Plant Science 8, 1950
| Early flowering as a drought escape mechanism in plants: how can it aid wheat production?Crossref | GoogleScholarGoogle Scholar | 29204147PubMed |
Shojaiefar S, Mirlohi A, Sabzalian MR, Yaghini H (2015) Seed yield and essential oil content of fennel influenced by genetic variation and genotype × year interaction. Industrial Crops and Products 71, 97–105.
| Seed yield and essential oil content of fennel influenced by genetic variation and genotype × year interaction.Crossref | GoogleScholarGoogle Scholar |
Singh G, Maurya S, de Lampasona MP, Catalan CA (2006) Chemical constituents, antifungal and antioxidative potential of Foeniculum vulgare volatile oil and its acetone extract. Food Control 17, 745–752.
| Chemical constituents, antifungal and antioxidative potential of Foeniculum vulgare volatile oil and its acetone extract.Crossref | GoogleScholarGoogle Scholar |
Singh Y, Mittal P (2003) Correlation and path-coefficient analysis in fennel (Foeniculum vulgare Mill.). Crop Research 25, 111–115.
Solouki M, Mehdikhani H, Zeinali H, Emamjomeh AA (2008) Study of genetic diversity in Chamomile (Matricaria chamomilla) based on morphological and molecular markers. Scientia Horticulturae 117, 281–287.
| Study of genetic diversity in Chamomile (Matricaria chamomilla) based on morphological and molecular markers.Crossref | GoogleScholarGoogle Scholar |
Steel RGD, Torrie JG (1960) ‘Principles and procedures of statistics: with special reference to the biological sciences’, 2nd edn. (McGraw–Hill: New York, NY, USA)
| Crossref |
Telci I, Demirtas I, Sahin A (2009) Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity. Industrial Crops and Products 30, 126–130.
| Variation in plant properties and essential oil composition of sweet fennel (Foeniculum vulgare Mill.) fruits during stages of maturity.Crossref | GoogleScholarGoogle Scholar |
Tognolini M, Ballabeni V, Bertoni S, Bruni R, Impicciatore M, Barocelli E (2007) Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis. Pharmacological Research 56, 254–260.
| Protective effect of Foeniculum vulgare essential oil and anethole in an experimental model of thrombosis.Crossref | GoogleScholarGoogle Scholar | 17709257PubMed |
Veronesi F, Falcinelli M (1988) Evaluation of an Italian germplasm collection of Festuca arundinacea Schreb. through a multivariate analysis. Euphytica 38, 211–220.
| Evaluation of an Italian germplasm collection of Festuca arundinacea Schreb. through a multivariate analysis.Crossref | GoogleScholarGoogle Scholar |
Yadav PS, Pandey VP, Yadav Y (2013) Variability studies in fennel (Foeniculum vulgare Mill.). Journal of Spices and Aromatic Crops 22, 203–208.
Yogi R, Meena RS, Kakani RK, Panwar A, Solanki RK (2013) Variability of some morphological characters in fennel (Foeniculum vulgare Mill.). International Journal of Seed Spices 3, 41–43.
Zahid Y, Abbasi A, Hafiz A, Ahmad Z (2008) Morphological characteristics and oil content of fennel (Foeniculum vulgare) accessions from different regions of Pakistan. Journal of the Chemical Society of Pakistan 30, 889–895.
Zarrabian M, Majidi MM, Ehtemam MH (2013) Genetic diversity in a worldwide collection of sainfoin using morphological, anatomical, and molecular markers. Crop Science 53, 2483–2496.
| Genetic diversity in a worldwide collection of sainfoin using morphological, anatomical, and molecular markers.Crossref | GoogleScholarGoogle Scholar |
