Mapping quantitative trait loci for cold tolerance at the booting stage in rice by using chromosome segment substitution lines
Jianguo Lei A C E , Shan Zhu A C E , Caihong Shao B E , Shusheng Tang C , Renliang Huang C , Changlan Zhu A D and Song Yan C D
+ Author Affiliations
- Author Affiliations
A Key Laboratory of Crop Physiology, Ecology and Genetic Breeding, Ministry of Education, Jiangxi Agricultural University, Nanchang 330045, Jiangxi Province, China.
B Soil and Fertilizer & Resources and Environment Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, Jiangxi Province, China.
C Rice National Engineering Laboratory (Nanchang)/Jiangxi Provincial Key Laboratory of Rice Physiology and Genetics/Rice Research Institute, Jiangxi Academy of Agricultural Sciences, Nanchang 330200, Jiangxi Province, China.
D Corresponding authors. Emails: yans11@163.com, zhuchanglan@163.com
E These authors contributed equally to this work.
Crop and Pasture Science 69(3) 278-283 https://doi.org/10.1071/CP17329
Submitted: 13 June 2017 Accepted: 22 November 2017 Published: 22 February 2018
Abstract
Low temperature at the booting stage in rice (Oryza sativa L.) can cause male sterility, resulting in yield losses. A set of chromosome segment substitution lines derived from the varieties Sasanishiki (cold-tolerant, ssp. japonica) and Habataki (cold-susceptible, ssp. indica) was used for analysis across two natural, low-temperature environments to study the genetic basis for cold tolerance at the booting stage. Spikelet fertility was used as the evaluation index for cold tolerance identification. Eight quantitative trait loci (QTLs) for cold tolerance were detected, two of which were located on chromosomes 3 (qCTSF3.1 and qCTSF3.2), and the others on chromosomes 4 (qCTSF4), 5 (qCTSF5), 6 (qCTSF6), 7 (qCTSF7), 8 (qCTSF8) and 9 (qCTSF9). The phenotypic variation explained by each QTL ranged from 5.4% to 25.3%. Of the eight QTLs, six (qCTSF3.2, qCTSF5, qCTSF6, qCTSF7, qCTSF8, qCTSF9) were repeatedly detected in two environments. QTLs qCTSF3.1, qCTSF7 and qCTSF9 overlapped with previously reported QTLs. All tolerant alleles for all QTLs were contributed by Sasanishiki.
Additional keywords: CSSL, genetic map, phenotyping.
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