Identification of drought-inducible regulatory factors in Lablab purpureus by a comparative genomic approach
Biao Wang A B E , Mingzhe Zhao C D E , Luming Yao A D , Maldonado dos Santos Joao V B , Valliyodan Babu B , Tianlong Wu A and Henry T. Nguyen B
+ Author Affiliations
- Author Affiliations
A Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China.
B National Center for Soybean Biotechnology and Division of Plant Sciences, University of Missouri,Columbia, MO 65211, USA.
C Agronomy College, Shenyang Agricultural University, Shenyang 110866, China.
D Corresponding authors. Emails: mzhao718@hotmail.com; lmyao@sjtu.edu.cn
E These authors contributed equally to the paper.
Crop and Pasture Science 69(6) 632-641 https://doi.org/10.1071/CP17236
Submitted: 1 July 2017 Accepted: 24 April 2018 Published: 5 June 2018
Abstract
Physiological indices of two lines of hyacinth bean (Lablab purpureus (L.) Sweet), MEIDOU 2012 and NANHUI 23, were compared during a progressive course of water-withholding treatments. MEIDOU 2012 was shown to be a superior drought-tolerant genotype. A suppressive subtractive hybridisation library was then constructed by using drought-stressed MEIDOU 2012 roots and sequenced to screen for transcripts with differential abundance variations. In total, 2792 unigenes were assembled from 4064 drought-induced expressed sequence tags. Comparative analysis with other legume genomic sequences, including soybean (Glycine max (L.) Merr.), common bean (Phaseolus vulgaris L.) and lucerne (Medicago sativa L.), was performed, from which 338 unigenes associated with root growth and drought response were identified. Among these unigenes, 27 transcription factors were deduced by functional-domain prediction. The transcription levels of a GRAS and a WRKY transcription factor were confirmed to be responding sensitively to water stresses. Additionally, protein–protein interaction (PPI) networks were predicted for all drought-induced root-related unigenes with different stringency levels. A mitogen-activated protein kinase (MAPK4) and an EF-hand calcium-binding protein (CML24) module were pinpointed to be putative ‘master’ signalling hubs in these PPI networks. Detailed examination of these genomic resources would further reveal key regulators of Lablab drought tolerance.
Additional keywords: EST, signaling, SSH.
References
Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Australian Journal of Biological Sciences 15, 413–428.| A re-examination of the relative turgidity technique for estimating water deficits in leaves.Crossref | GoogleScholarGoogle Scholar |
Bhatnagar-Mathur P, Devi MJ, Vadez V, Sharma KK (2009) Differential antioxidative responses in transgenic peanut bear no relationship to their superior transpiration efficiency under drought stress. Journal of Plant Physiology 166, 1207–1217.
| Differential antioxidative responses in transgenic peanut bear no relationship to their superior transpiration efficiency under drought stress.Crossref | GoogleScholarGoogle Scholar |
Blair MW, Fernandez AC, Ishitani M, Moreta D, Seki M, Ayling S, Shinozaki K (2011) Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.). BMC Plant Biology 11, 171
| Construction and EST sequencing of full-length, drought stress cDNA libraries for common beans (Phaseolus vulgaris L.).Crossref | GoogleScholarGoogle Scholar |
Buege JA, Aust SD (1978) Microsomal lipid peroxidation methods. Enzymology 52, 302–310.
| Microsomal lipid peroxidation methods.Crossref | GoogleScholarGoogle Scholar |
Cameron DG (1988) Tropical and subtropical pasture legumes. Queensland Agricultural Journal 114, 110–113.
Condon AG, Richards RA, Rebetzke GJ, Farquhar GD (2002) Improving intrinsic water use efficiency and crop yield. Crop Science 42, 122–131.
| Improving intrinsic water use efficiency and crop yield.Crossref | GoogleScholarGoogle Scholar |
D’Souza MR, Devaraj VR (2011) Specific and non-specific responses of Hyacinth bean (Dolichos lablab) to drought stress. Indian Journal of Biotechnology 10, 130–139.
de Mezer M, Turska-Taraska A, Kaczmarek Z, Glowacka K, Swarcewicz B, Rorat T (2014) Differential physiological and molecular response of barley genotypes to water deficit. Plant Physiology and Biochemistry 80, 234–248.
| Differential physiological and molecular response of barley genotypes to water deficit.Crossref | GoogleScholarGoogle Scholar |
Delk NA, Johnson KA, Chowdhury NI, Braam J (2005) CML24, regulated in expression by diverse stimuli, encodes a potential Ca2+ sensor that functions in responses to abscisic acid, daylength, and ion stress. Plant Physiology 139, 240–253.
| CML24, regulated in expression by diverse stimuli, encodes a potential Ca2+ sensor that functions in responses to abscisic acid, daylength, and ion stress.Crossref | GoogleScholarGoogle Scholar |
Deokar AA, Kondawar V, Jain PK, Karuppayil SM, Raju NL, Vadez V, Varshney RK, Srinivasan R (2011) Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress. BMC Plant Biology 11, 70
| Comparative analysis of expressed sequence tags (ESTs) between drought-tolerant and -susceptible genotypes of chickpea under terminal drought stress.Crossref | GoogleScholarGoogle Scholar |
Deshmukh R, Sonah H, Patil G, Chen W, Prince S, Mutava R, Vuong T, Valliyodan B, Nguyen HT (2014) Integrating genomic approaches for abiotic stress tolerance in soybean. Frontiers in Plant Science 5, 244
| Integrating genomic approaches for abiotic stress tolerance in soybean.Crossref | GoogleScholarGoogle Scholar |
Golldack D, Lüking I, Yang O (2011) Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Reports 30, 1383–1391.
| Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network.Crossref | GoogleScholarGoogle Scholar |
Guretzki S, Papenbrock J (2014) Characterization of Lablab purpureus regarding drought tolerance, trypsin inhibitor activity and cyanogenic potential for selection in breeding programmes. Journal of Agronomy & Crop Science 200, 24–35.
| Characterization of Lablab purpureus regarding drought tolerance, trypsin inhibitor activity and cyanogenic potential for selection in breeding programmes.Crossref | GoogleScholarGoogle Scholar |
Hameed A, Bibi N, Akhter J, Iqbal N (2011) Differential changes in antioxidants, proteases, and lipid peroxidation in flag leaves of wheat genotypes under different levels of water deficit conditions. Plant Physiology and Biochemistry 49, 178–185.
| Differential changes in antioxidants, proteases, and lipid peroxidation in flag leaves of wheat genotypes under different levels of water deficit conditions.Crossref | GoogleScholarGoogle Scholar |
Harb A, Krishnan A, Ambavaram MMR, Pereira A (2010) Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth. Plant Physiology 154, 1254–1271.
| Molecular and physiological analysis of drought stress in Arabidopsis reveals early responses leading to acclimation in plant growth.Crossref | GoogleScholarGoogle Scholar |
Hendricksen RE, Minson DJ (1985) Lablab purpureus—A review. Herbage Abstracts 55, 215–227.
Hotti AH, Naik VR, Makanur B, Mahiboobsa M (2013) Study on yield, yield attributes and grain protein of field bean genotypes. Plant Archives 13, 827–830.
Huang X, Madan A (1999) CAP3: A DNA sequence assembly program. Genome Research 9, 868–877.
Kang Y, Han Y, Torres-Jerez I, Wang M, Tang Y, Monteros M, Udvardi M (2011) System responses to long-term drought and re-watering of two contrasting alfalfa varieties. The Plant Journal 68, 871–889.
| System responses to long-term drought and re-watering of two contrasting alfalfa varieties.Crossref | GoogleScholarGoogle Scholar |
Kilian J, Peschke F, Berendzen KW, Harter K, Wanke D (2012) Prerequisites, performance and profits of transcriptional profiling the abiotic stress response. Biochimica et Biophysica Acta 1819, 166–175.
| Prerequisites, performance and profits of transcriptional profiling the abiotic stress response.Crossref | GoogleScholarGoogle Scholar |
Kimani EN, Wachira FN, Kinyua MG (2012) Molecular diversity of Kenyan Lablab bean (Lablab purpureus (L.) Sweet) accessions using amplified fragment length polymorphism markers. American Journal of Plant Sciences 3, 313–321.
| Molecular diversity of Kenyan Lablab bean (Lablab purpureus (L.) Sweet) accessions using amplified fragment length polymorphism markers.Crossref | GoogleScholarGoogle Scholar |
Knievel DP (1973) Procedures for estimating ratio of live or dry matter in root samples. Crop Science 13, 124–126.
| Procedures for estimating ratio of live or dry matter in root samples.Crossref | GoogleScholarGoogle Scholar |
Kokila S, Devaraj VR (2015) Evaluation of Hyacinth bean (Lablab purpureus) genotypes for salinity-tolerance. Research Journal of Biotechnology 6, 73–82.
Li MM, Li YH, Zhao JY, Liu H, Jia SH, Li J, Zhao HP, Han SC, Wang YD (2016) GpDSR7, a novel E3 Ubiquitin ligase gene in Grimmia pilifera is involved in tolerance to drought stress in Arabidopsis. PLoS One 11, e0155455
| GpDSR7, a novel E3 Ubiquitin ligase gene in Grimmia pilifera is involved in tolerance to drought stress in Arabidopsis.Crossref | GoogleScholarGoogle Scholar |
Lindemose S, O’Shea C, Jensen MK, Skriver K (2013) Structure, function and networks of transcription factors involved in abiotic stress responses. Biochimica et Biophysica Acta 14, 5842–5878.
Ma W, Smigel A, Tsai YC, Braam J, Berkowitz GA (2008) Innate immunity signaling: cytosolic Ca2+ elevation is linked to downstream nitric oxide generation through the action of calmodulin or a calmodulin-like protein. Plant Physiology 148, 818–828.
| Innate immunity signaling: cytosolic Ca2+ elevation is linked to downstream nitric oxide generation through the action of calmodulin or a calmodulin-like protein.Crossref | GoogleScholarGoogle Scholar |
Maass BL, Jamnadass RH, Hanson J, Pengelly BC (2005) Determining sources of diversity in cultivated and wild Lablab purpureus related to provenance of germplasm by using amplified fragment length polymorphism. Genetic Resources and Crop Evolution 52, 683–695.
| Determining sources of diversity in cultivated and wild Lablab purpureus related to provenance of germplasm by using amplified fragment length polymorphism.Crossref | GoogleScholarGoogle Scholar |
Mayer L, Chandler DR, Taylor MS (1986) Lablab purpureus—a fodder crop for Botswana. Bulletin of Agricultural Research in Botswana 5, 37–48.
McMichael BL, Elmore CD (1977) Proline accumulation in water stressed cotton leaves. Crop Science 17, 905–908.
| Proline accumulation in water stressed cotton leaves.Crossref | GoogleScholarGoogle Scholar |
Murphy AM, Colucci EA (1999) Tropical forage solution to poor quality ruminant diets: A review of Lablab purpureus. Livestock Research for Rural Development 11, 96–113.
Olsen AN, Ernst HA, Leggio LL, Skriver K (2005) NAC transcription factors: structurally distinct, functionally diverse. Trends in Plant Science 10, 79–87.
| NAC transcription factors: structurally distinct, functionally diverse.Crossref | GoogleScholarGoogle Scholar |
Palm-Forster MAT, Eschen-Lippold L, Uhrig J, Scheel D, Lee J (2017) A novel family of proline/serine-rich proteins, which are phospho-targets of stress-related mitogen-activated protein kinases, differentially regulates growth and pathogen defense in Arabidopsis thaliana. Plant Molecular Biology 95, 123–140.
| A novel family of proline/serine-rich proteins, which are phospho-targets of stress-related mitogen-activated protein kinases, differentially regulates growth and pathogen defense in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar |
Pandey N, Ranjan A, Pant P, Tripathi RK, Ateek F, Pandey HP, Patre UV, Sawant SV (2013) CAMTA 1 regulates drought responses in Arabidopsis thaliana. BMC Genomics 14, 216
| CAMTA 1 regulates drought responses in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar |
Pandey MK, Roorkiwal M, Singh VK, Ramalingam A, Kudapa H, Thudi M, Chitikineni A, Rathore A, Varshney RK (2016) Emerging genomic tools for legume breeding: current status and future prospects. Frontiers in Plant Science 7, 455
| Emerging genomic tools for legume breeding: current status and future prospects.Crossref | GoogleScholarGoogle Scholar |
Ramamoorthy R, Jiang SY, Kumar N, Venkatesh PN, Ramachandran SA (2008) Comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments. Plant & Cell Physiology 49, 865–879.
| Comprehensive transcriptional profiling of the WRKY gene family in rice under various abiotic and phytohormone treatments.Crossref | GoogleScholarGoogle Scholar |
Ranty B, Aldon D, Cotelle V, Galaud JP, Thuleau P, Mazars C (2016) Calcium sensors as key hubs in plant responses to biotic and abiotic stresses. Frontiers in Plant Science 7, 327
| Calcium sensors as key hubs in plant responses to biotic and abiotic stresses.Crossref | GoogleScholarGoogle Scholar |
Shehu Y, Alhassan WS, Pal UR, Phillips CJC (2001) The effects of plant population density on the growth and chemical composition of Lablab purpureus grown for fodder production in a semi-arid region. Journal of Agronomy & Crop Science 186, 83–89.
| The effects of plant population density on the growth and chemical composition of Lablab purpureus grown for fodder production in a semi-arid region.Crossref | GoogleScholarGoogle Scholar |
Sofo A, Dichio B, Xiloyannis C, Masia A (2004) Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress. Physiologia Plantarum 121, 58–65.
| Lipoxygenase activity and proline accumulation in leaves and roots of olive trees in response to drought stress.Crossref | GoogleScholarGoogle Scholar |
Talukdar D (2013) Comparative morpho-physiological and biochemical responses of lentil and grass pea genotypes under water stress. Journal of Natural Science, Biology, and Medicine 4, 396–402.
| Comparative morpho-physiological and biochemical responses of lentil and grass pea genotypes under water stress.Crossref | GoogleScholarGoogle Scholar |
Yao L, Wang B, Cheng L, Wu T (2013) Identification of key drought stress-related genes in the hyacinth bean. PLoS One 8, e58108
| Identification of key drought stress-related genes in the hyacinth bean.Crossref | GoogleScholarGoogle Scholar |
Zeng H, Xu L, Singh A, Wang H, Du L, Poovaiah BW (2015) Involvement of calmodulin and calmodulin-like proteins in plant responses to abiotic stresses. Frontiers in Plant Science 6, 600
| Involvement of calmodulin and calmodulin-like proteins in plant responses to abiotic stresses.Crossref | GoogleScholarGoogle Scholar |
Zhang ZZ, Ma ZQ, Liu DJ (2005) Suppression subtractive hybridization analysis of Ms2 near-isogenic lines of wheat reveals genes differentially expressed in spikelets and anthers. Journal of Plant Physiology and Molecular Biology 31, 175–182.
