Development of an ultrasound-assisted extraction method for the rapid quantification of seed carotenoid content in oilseed rape
Lei Xue A , Fang Wei A , Guizhen Gao A , Guixin Yan A , Weilin Song A , Biyun Chen A , Kun Xu A , Hong Chen A and Xiaoming Wu A B
+ Author Affiliations
- Author Affiliations
A Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, P. R. China.
B Corresponding author. Email: wuxm@oilcrops.cn
Crop and Pasture Science 69(1) 48-54 https://doi.org/10.1071/CP16351
Submitted: 22 September 2016 Accepted: 4 July 2017 Published: 11 August 2017
Abstract
The selection of cultivars with high carotenoid content represents a novel goal in the genetic improvement of oilseed rape. The successful development of an efficient method for the quantification of carotenoids in oilseed rape is a prerequisite for this breeding initiative. In the present study, ultrasound-assisted extraction was combined with a spectrophotometric method to quantify seed carotenoid levels in oilseed rape. The key variables were optimised as follows: the solvent used was a 1 : 1 (v : v) mixture of petroleum ether and acetone, the solid to solvent concentration was 0.08 g/mL, the ultrasound frequency and power were set at 45/100 (kHZ/w), and three extractions of 15 min each were performed. Compared with the conventional solvent extraction method, the time required for extraction using the ultrasound-assisted extraction method was significantly shortened (by 24 times), and the reproducibility was also remarkably improved. These results indicate that ultrasound-assisted spectrophotometric quantification is a rapid, accurate, simple and low cost tool for the screening and selection of rapeseed cultivars with high carotenoid content.
Additional keywords: quality breeding, spectrophotometric quantification.
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