Molecular characterisation of novel LMW-m and LMW-s genes from four Aegilops species (Sitopsis section) and comparison with those from the Glu-B3 locus of common wheat
Susana Cuesta A , Carlos Guzmán B and Juan B. Alvarez A C
+ Author Affiliations
- Author Affiliations
A Departamento de Genética, Escuela Técnica Superior de Ingeniería Agronómica y de Montes, Edificio Gregor Mendel, Campus de Rabanales, Universidad de Córdoba, CeiA3, ES-14071 Córdoba, Spain.
B Wheat Chemistry and Quality Laboratory, Global Wheat Program, International Maize and Wheat Improvement Center (CIMMYT), Texcoco, Mexico.
C Corresponding author. Email: jb.alvarez@uco.es
Crop and Pasture Science 67(9) 938-947 https://doi.org/10.1071/CP16185
Submitted: 22 May 2016 Accepted: 20 July 2016 Published: 2 September 2016
Abstract
Low-molecular-weight glutenin subunits (LMW-GS) are a component of the gluten network and play a key role in determining the viscoelastic properties of wheat dough. Aegilops species have been shown to be an important source of variation for valuable traits for wheat breeding. However, very little is known about LMW-GS genes in section Sitopsis species, which are closely related to the B genome of common wheat. Ten accessions of Sitopsis species were evaluated for variability of LMW-GS genes, and 20 novel genes were obtained, of which nine were LMW-m and 11 were LMW-s genes. Only two were pseudogenes, corresponding to one LMW-m and one LMW-s gene. Six groups of genes were detected: three for each of the LMW-m and LMW-s genes. All groups of LMW-s genes and one of LMW-m genes (pGluU) detected were not related to B-genome genes from common wheat, whereas the remaining genes were. The single-nucleotide polymorphisms, and insertions and deletions, detected in active variants compared with those from common wheat could affect structure protein. The analysis of reactive epitopes for coeliac disease revealed that LMW-s subunits lacked toxicity, as did the pGluU LMW-m subunits; the other LMW-m subunits were less toxic than that from common wheat.
Additional keywords: Aegilops sp., coeliac disease, gluten quality, InDel, SNP.
References
Chen P, Li R, Zhou R, Zhiguo E, He G (2010) Cloning and characterization of novel low molecular weight glutenin subunit genes from two Aegilops species with the C and D genomes. Genetic Resources and Crop Evolution 57, 881–890.| Cloning and characterization of novel low molecular weight glutenin subunit genes from two Aegilops species with the C and D genomes.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXos1eqsrk%3D&md5=3ba8c00e729c7da7acc0f48459937676CAS |
Chen F, Zhao F, Liu R, Xia G (2011) Functional properties of two low-molecular-weight glutenin subunits carrying additional cysteine residues from hybrid introgression line II-12 derived from Triticum aestivum and Agropyron elongatum. Food Chemistry 127, 1773–1776.
| Functional properties of two low-molecular-weight glutenin subunits carrying additional cysteine residues from hybrid introgression line II-12 derived from Triticum aestivum and Agropyron elongatum.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXjvVWjsL8%3D&md5=3e683d9b949bc67a9658c8a954d95358CAS |
Cuesta S, Guzmán C, Alvarez JB (2015) Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil. Theoretical and Applied Genetics 128, 2155–2165.
| Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhtFGlu7nL&md5=3c3b556a9fb196ea1a44494f56824f1fCAS | 26152575PubMed |
D’Ovidio R, Masci S (2004) The low-molecular-weight glutenin subunits of wheat gluten. Journal of Cereal Science 39, 321–339.
| The low-molecular-weight glutenin subunits of wheat gluten.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXksVantL0%3D&md5=0b8f22eef58d8bdece901d50fe75ccc0CAS |
Dong L, Zhang X, Liu D, Fan H, Sun J, Zhang Z, Qin H, Li B, Hao S, Li Z, Wang D, Zhang A, Ling HQ (2010) New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat. PLoS One 5, e13548
| New insights into the organization, recombination, expression and functional mechanism of low molecular weight glutenin subunit genes in bread wheat.Crossref | GoogleScholarGoogle Scholar | 20975830PubMed |
Egidi E, Sestili F, Janni M, D’Ovidio R, Lafiandra D, Ceriotti A, Vensel WH, Kasarda DD, Masci S (2014) An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm. BMC Plant Biology 14, 64
| An asparagine residue at the N-terminus affects the maturation process of low molecular weight glutenin subunits of wheat endosperm.Crossref | GoogleScholarGoogle Scholar | 24629124PubMed |
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
| Confidence limits on phylogenies: an approach using the bootstrap.Crossref | GoogleScholarGoogle Scholar |
Guzmán C, Caballero L, Martín LM, Alvarez JB (2012) Waxy genes from spelt wheat: new alleles for modern wheat breeding and new phylogenetic inferences about the origin of this species. Annals of Botany 110, 1161–1171.
| Waxy genes from spelt wheat: new alleles for modern wheat breeding and new phylogenetic inferences about the origin of this species.Crossref | GoogleScholarGoogle Scholar | 22984164PubMed |
Haider N (2013) The origin of the B-genome of bread wheat (Triticum aestivum L.). Russian Journal of Genetics 49, 263–274.
| The origin of the B-genome of bread wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXksFOiurw%3D&md5=066352914058aaf206311a2d0312ace6CAS |
Huang X-Q, Cloutier S (2008) Molecular characterization and genomic organization of low molecular weight glutenin subunit genes at the Glu-3 loci in hexaploid wheat (Triticum aestivum). Theoretical and Applied Genetics 116, 953–966.
| Molecular characterization and genomic organization of low molecular weight glutenin subunit genes at the Glu-3 loci in hexaploid wheat (Triticum aestivum).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXkvFylsbg%3D&md5=298f5e558084293c5f51b94d67d6e5e1CAS | 18305921PubMed |
Huang S, Sirikhachornkit A, Su X, Faris J, Gill B, Haselkorn R, Gornicki P (2002) Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat. Proceedings of the National Academy of Sciences of the United States of America 99, 8133–8138.
| Genes encoding plastid acetyl-CoA carboxylase and 3-phosphoglycerate kinase of the Triticum/Aegilops complex and the evolutionary history of polyploid wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XkvVGju78%3D&md5=54c8b2718420a6f686cd23065c4ba5d9CAS | 12060759PubMed |
Huang Z, Long H, Jiang QT, Wei YM, Yan ZH, Zheng YL (2010) Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima. Journal of Applied Genetics 51, 9–18.
| Molecular characterization of novel low-molecular-weight glutenin genes in Aegilops longissima.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BC3c7gt1eltQ%3D%3D&md5=186a02e039327b5a1338b840d8e2eb9bCAS | 20145295PubMed |
Ikeda TM, Nagamine T, Fukuoka H, Yano H (2002) Identification of new low-molecular-weight glutenin subunit genes in wheat. Theoretical and Applied Genetics 104, 680–687.
| Identification of new low-molecular-weight glutenin subunit genes in wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XjtlSqtb4%3D&md5=f1d0055fe93b528332b2838f49675858CAS | 12582674PubMed |
Jiang C, Pei Y, Zhang Y, Li X, Yao D, Yan Y, Ma W, Hsam SLK, Zeller FJ (2008) Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi. Hereditas 145, 92–98.
| Molecular cloning and characterization of four novel LMW glutenin subunit genes from Aegilops longissima, Triticum dicoccoides and T. zhukovskyi.Crossref | GoogleScholarGoogle Scholar | 18503711PubMed |
Johal J, Gianibelli MC, Rahman S, Morell MK, Gale KR (2004) Characterization of low-molecular-weight glutenin genes in Aegilops tauschii. Theoretical and Applied Genetics 109, 1028–1040.
| Characterization of low-molecular-weight glutenin genes in Aegilops tauschii.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXotlKqt7s%3D&md5=a7fa385f318585d0e7cf2fb900d29cd7CAS | 15164175PubMed |
Li XH, Wang K, Wang SL, Gao LY, Xie XX, Hsam SLK, Zeller FJ, Yan YM (2010) Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species. Theoretical and Applied Genetics 121, 845–856.
| Molecular characterization and comparative transcriptional analysis of LMW-m-type genes from wheat (Triticum aestivum L.) and Aegilops species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtVahtrnO&md5=1c73b8ae0b2e59d326530dee0af9392fCAS | 20490445PubMed |
Long H, Huang Z, Wei YM, Yan ZH, Ma ZC, Zheng YL (2008) Length variation of i-type low-molecular-weight glutenin subunit genes in diploid wheats. Russian Journal of Genetics 44, 429–435.
| Length variation of i-type low-molecular-weight glutenin subunit genes in diploid wheats.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXltlCrtLg%3D&md5=effb00b607971fcca2c20cb43c03dbc4CAS |
Luo G, Zhang X, Zhang Y, Yang W, Li Y, Sun J, Zhan K, Zhang A, Liu D (2015) Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu. BMC Plant Biology 15, 68
| Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu.Crossref | GoogleScholarGoogle Scholar | 25849991PubMed |
Ma ZC, Wei YM, Long H, Yan ZH, Baum B, Zheng YL (2006) Characterization of low-molecular-weight i-type glutenin subunit genes from diploid wheat in relation to the gene family structure. Molecular Biology 40, 897–906.
| Characterization of low-molecular-weight i-type glutenin subunit genes from diploid wheat in relation to the gene family structure.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlShsL%2FP&md5=58eeb121aa9ff15d63be1ff5053c5ce2CAS |
Masci S, D’Ovidio R, Lafiandra D, Kasarda DD (1998) Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer. Plant Physiology 118, 1147–1158.
| Characterization of a low-molecular-weight glutenin subunit gene from bread wheat and the corresponding protein that represents a major subunit of the glutenin polymer.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXnsQ%3D%3D&md5=028f0b4e08b4c80e79ee4742c719f74bCAS | 9847089PubMed |
Masci S, D’Ovidio R, Lafiandra D, Kasarda DD (2000) A 1B-coded low-molecular-weight glutenin subunit associated with quality in durum wheats shows strong similarity to a subunit present in some bread wheat cultivars. Theoretical and Applied Genetics 100, 396–400.
| A 1B-coded low-molecular-weight glutenin subunit associated with quality in durum wheats shows strong similarity to a subunit present in some bread wheat cultivars.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXisVaktrw%3D&md5=9459ebe21a0e5e98a4e7e6628642ddf2CAS |
Ortega R, Alvarez JB, Guzmán C (2014) Characterization of the Wx gene in diploid Aegilops species and its potential use in wheat breeding. Genetic Resources and Crop Evolution 61, 369–382.
| Characterization of the Wx gene in diploid Aegilops species and its potential use in wheat breeding.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXitFChs7Y%3D&md5=a996428118ce8f44848ee37dd4775ce3CAS |
Pei Y-H, Wang A-L, An X-L, Li X-H, Zhang Y-Z, Huang X-Q, Yan Y-M (2007) Characterization and comparative analysis of three low molecular weight glutenin C-subunit genes isolated from Aegilops tauschii. Canadian Journal of Plant Science 87, 273–280.
| Characterization and comparative analysis of three low molecular weight glutenin C-subunit genes isolated from Aegilops tauschii.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXms1Oksrc%3D&md5=eb3e2e3d5f34de1307aaec52baba094fCAS |
Petersen G, Seberg O, Yde M, Berthelsen K (2006) Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum). Molecular Phylogenetics and Evolution 39, 70–82.
| Phylogenetic relationships of Triticum and Aegilops and evidence for the origin of the A, B, and D genomes of common wheat (Triticum aestivum).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XisF2ht78%3D&md5=e67c1526e6f1b05a765a0f1d4214d914CAS | 16504543PubMed |
Pogna NE, Autran JC, Mellini F, Lafiandra D, Feillet P (1990) Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength. Journal of Cereal Science 11, 15–34.
| Chromosome 1B-encoded gliadins and glutenin subunits in durum wheat: genetics and relationship to gluten strength.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXhtlKis78%3D&md5=29ee434b424c565733622d6684283872CAS |
Rasheed A, Xia X, Yan Y, Appels R, Mahmood T, He Z (2014) Wheat seed storage proteins: Advances in molecular genetics, diversity and breeding applications. Journal of Cereal Science 60, 11–24.
| Wheat seed storage proteins: Advances in molecular genetics, diversity and breeding applications.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXksleht7o%3D&md5=2c0cb5f935d9b109e9f33754aa55ce19CAS |
Sarkar P, Stebbins GL (1956) Morphological evidence concerning the origin of the B genome in wheat. American Journal of Botany 43, 297–304.
| Morphological evidence concerning the origin of the B genome in wheat.Crossref | GoogleScholarGoogle Scholar |
Schneider A, Molnár I, Molnár-Láng M (2008) Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163, 1–19.
| Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXovFyit7Y%3D&md5=58c05d4870dbc2f2ee0e0d07a301e038CAS |
Singh NK, Shepherd KW (1988) Linkage mapping of genes controlling endosperm storage proteins in wheat. Theoretical and Applied Genetics 75, 628–641.
| Linkage mapping of genes controlling endosperm storage proteins in wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXkt1SgtL4%3D&md5=7f5914372d6594749f5f184870e8f60bCAS |
Stacey J, Isaac P (1994) Isolation of DNA from plants. In ‘Methods in molecular biology: Protocols for nucleic acid analysis by non-radioactive probes’. (Ed. PG Isaac) pp. 9–15. (Humana Press: New York)
Tamura K, Nei M, Kumar S (2004) Prospects for inferring very large phylogenies by using the neighbor-joining method. Proceedings of the National Academy of Sciences of the United States of America 101, 11030–11035.
| Prospects for inferring very large phylogenies by using the neighbor-joining method.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXmsVCmt7s%3D&md5=057aa843fdcdf8fb3e7b2fbe95c413eeCAS | 15258291PubMed |
Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution 28, 2731–2739.
| MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXht1eiu73K&md5=d9be6c4e83537dc5115659945509845dCAS | 21546353PubMed |
Tanaka H, Shimizu R, Tsujimoto H (2005) Genetical analysis of contribution of low-molecular-weight glutenin subunits to dough strength in common wheat (Triticum aestivum L.). Euphytica 141, 157–162.
| Genetical analysis of contribution of low-molecular-weight glutenin subunits to dough strength in common wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXjt1Wgu7w%3D&md5=13ab579e57bf386d5bb09687ca82a1a9CAS |
Vader W, Kooy Y, van Veelen P, de Ru A, Harris D, Benckhuijsen W, Peña S, Mearin L, Drijfhout JW, Koning F (2002) The gluten response in children with celiac disease is directed toward multiple gliadin and glutenin peptides. Gastroenterology 122, 1729–1737.
| The gluten response in children with celiac disease is directed toward multiple gliadin and glutenin peptides.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xlt12gsrY%3D&md5=7066181276635bcc7b114a2284b5732cCAS | 12055577PubMed |
Wang LH, Zhao XL, He ZH, Ma W, Appels R, Peña RJ, Xia XC (2009) Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 118, 525–539.
| Characterization of low-molecular-weight glutenin subunit Glu-B3 genes and development of STS markers in common wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXpt1CltQ%3D%3D&md5=432b2d72afcfd810d5e798c383f6391fCAS | 18989655PubMed |
Wang LH, Zhou M, Li HL, He ZH, Xia XC (2010) Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species. Hereditas 32, 613–624.
| Cloning and phylogenetic analysis of low-molecular-weight glutenin subunit genes at Glu-B3 locus in common wheat relative species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlGhs7jO&md5=71331ad13d5e03152c6a2d7445f454e0CAS | 20566466PubMed |
Wang K, Gao L, Wang S, Zhang Y, Li X, Zhang M, Xie Z, Yan Y, Belgard M, Ma W (2011a) Phylogenetic relationship of a new class of LMW-GS genes in the M genome of Aegilops comosa. Theoretical and Applied Genetics 122, 1411–1425.
| Phylogenetic relationship of a new class of LMW-GS genes in the M genome of Aegilops comosa.Crossref | GoogleScholarGoogle Scholar | 21301802PubMed |
Wang S, Li X, Wang K, Wang X, Li S, Zhang Y, Guo G, Zeller FJ, Hsam SLK, Yan Y, Gustafson P (2011b) Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci. Genome 54, 273–284.
| Phylogenetic analysis of C, M, N, and U genomes and their relationships with Triticum and other related genomes as revealed by LMW-GS genes at Glu-3 loci.Crossref | GoogleScholarGoogle Scholar | 21491971PubMed |
Wrigley CW, Békés F, Bushuk W (Eds) (2006) ‘Gliadin and glutenin: The unique balance of wheat quality.’ (AACC International Press: St. Paul, MN, USA)
Zhang X, Liu D, Jiang W, Guo X, Yang W, Sun J, Ling H, Zhang A (2011) PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 123, 1293–1305.
| PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3MXhsVylu7zI&md5=ab8cc0a673852b4f6a63920401b31cd9CAS | 21830110PubMed |
Zhang X, Liu D, Zhang J, Jiang W, Luo G, Yang W, Sun J, Tong Y, Cui D, Zhang A (2013) Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat. Journal of Experimental Botany 64, 2027–2040.
| Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXmslSltbc%3D&md5=2b5d754d85e33e9c906364e632ba81e2CAS | 23536608PubMed |
Zhao X, Yang Y, He Z, Lei Z, Ma W, Sun Q, Xia X (2008) Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii. Hereditas 145, 238–250.
| Characterization of novel LMW-GS genes at Glu-D3 locus on chromosome 1D in Aegilops tauschii.Crossref | GoogleScholarGoogle Scholar | 19076692PubMed |
