Molecular basis of multiple resistance to herbicides inhibiting acetyl-CoA carboxylase and acetolactate synthase in American sloughgrass (Beckmannia syzigachne) from China
Lang Pan A B , Haitao Gao A B , Han Wu A B and Liyao Dong A B C
+ Author Affiliations
- Author Affiliations
A College of Plant Protection, Nanjing Agricultural University, Nanjing 210095, China.
B Key Laboratory of Integrated Pest Management in Crops in Eastern China (Ministry of Agriculture), Nanjing Agricultural University, Nanjing 210095, China.
C Corresponding author. Email: dly@njau.edu.cn
Crop and Pasture Science 67(11) 1208-1214 https://doi.org/10.1071/CP16109
Submitted: 24 March 2016 Accepted: 5 September 2016 Published: 7 October 2016
Abstract
American sloughgrass (Beckmannia syzigachne Steud.) is a problematic grass that is widely distributed in wheat and oilseed rape fields in China. The herbicides fenoxaprop-P-ethyl and mesosulfuron-methyl failed to control B. syzigachne JCWJ-R populations collected from a wheat field in Jiangsu Province. Dose-response experiments showed that JCWJ-R was resistant to the acetyl-CoA carboxylase (ACCase) inhibitors fenoxaprop-P-ethyl (33.8-fold), haloxyfop-R-methyl (12.7-fold), clethodim (7.8-fold) and pinoxaden (11.6-fold), and to the acetolactate synthase (ALS) inhibitors mesosulfuron-methyl (15.9-fold), pyroxsulam (17.6-fold), flucarbazone-Na (10.7-fold) and imazethapyr (7-fold). Resistance to ALS inhibitors was due to a Pro-197-Ser mutation in the ALS gene and resistance to ACCase inhibitors was due to an Ile-1781-Leu mutation in the ACCase gene. A derived cleaved amplified polymorphic sequence method was developed to detect the ALS mutation in B. syzigachne. This was combined with a previously established method to detect Ile-1781-Leu, and the mutation frequency and homozygous mutation rates in the JCWJ-R population were determined. The evolution of multiple resistance to ACCase and ALS inhibitors in this B. syzigachne population indicated that alternative methods should be developed to control resistant weeds.
Additional keywords: amino acid substitution, cross-resistance, herbicide resistance, target-site resistance.
References
Beckie HJ, Tardif FJ (2012) Herbicide cross resistance in weeds. Crop Protection 35, 15–28.| Herbicide cross resistance in weeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XjsVCgsrY%3D&md5=a58d45bab35f87aaa1ae602ccd4e4a41CAS |
Bi YL, Liu WT, Li LX, Yuan GH, Jin T, Wang JX (2013) Molecular basis of resistance to mesosulfuron-methyl in Japanese foxtail, Alopecurus japonicus. Journal of Pesticide Science 38, 74–77.
| Molecular basis of resistance to mesosulfuron-methyl in Japanese foxtail, Alopecurus japonicus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhtVSiurrF&md5=6a7408a58c060e233558a9398cbe7c4fCAS |
Bi Y, Liu W, Guo W, Li L, Yuan G, Du L, Wang J (2016) Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China. Pesticide Biochemistry and Physiology 126, 22–27.
| Molecular basis of multiple resistance to ACCase- and ALS-inhibiting herbicides in Alopecurus japonicus from China.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXht1OqtLfI&md5=fe93e330f75c416790c45a0d3cc646c1CAS | 26778430PubMed |
Collavo A, Strek H, Beffa R, Sattin M (2013) Management of an ACCase-inhibitor-resistant Lolium rigidum population based on the use of ALS inhibitors: weed population evolution observed over a 7 year field-scale investigation. Pest Management Science 69, 200–208.
| Management of an ACCase-inhibitor-resistant Lolium rigidum population based on the use of ALS inhibitors: weed population evolution observed over a 7 year field-scale investigation.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38Xhsl2lu7vI&md5=47fe50ffb2e43abca6cf58f880ee2a48CAS | 23225328PubMed |
Délye C (2013) Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade. Pest Management Science 69, 176–187.
| Unravelling the genetic bases of non-target-site-based resistance (NTSR) to herbicides: a major challenge for weed science in the forthcoming decade.Crossref | GoogleScholarGoogle Scholar | 22614948PubMed |
Guo WL, Liu WT, Li LX, Yuan GH, Du L, Wang JX (2015) Molecular basis for resistance to fenoxaprop in shortawn foxtail (Alopecurus aequalis) from China. Weed Science 63, 416–424.
| Molecular basis for resistance to fenoxaprop in shortawn foxtail (Alopecurus aequalis) from China.Crossref | GoogleScholarGoogle Scholar |
Hamouzová K, Košnarová P, Salava J, Soukup J, Hamouz P (2014) Mechanisms of resistance to acetolactate synthase-inhibiting herbicides in populations of Apera spica-venti from the Czech Republic. Pest Management Science 70, 541–548.
| Mechanisms of resistance to acetolactate synthase-inhibiting herbicides in populations of Apera spica-venti from the Czech Republic.Crossref | GoogleScholarGoogle Scholar | 23893862PubMed |
Heap I (2016) International Survey of Herbicide Resistant Weeds. Available at: www.weedscience.org (accessed 26 August 2016).
Kaundun SS (2014) Resistance to acetyl-CoA carboxylase-inhibiting herbicides. Pest Management Science 70, 1405–1417.
| Resistance to acetyl-CoA carboxylase-inhibiting herbicides.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXnsVWqt7k%3D&md5=a2c2ecf73a94ce5dc2f23173cdc8d78dCAS | 24700409PubMed |
Kaundun SS, Bailly GC, Dale RP, Hutchings SJ, McIndoe E (2013a) A novel W1999S mutation and non-target site resistance impact on acetyl-CoA carboxylase inhibiting herbicides to varying degrees in a UK Lolium multiflorum population. PLoS One 8, e58012
| A novel W1999S mutation and non-target site resistance impact on acetyl-CoA carboxylase inhibiting herbicides to varying degrees in a UK Lolium multiflorum population.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXktV2jtbg%3D&md5=d23a6c7d7d042b0e9e1798c5c28076c4CAS | 23469130PubMed |
Kaundun SS, Hutchings SJ, Dale RP, McIndoe E (2013b) Role of a novel I1781T mutation and other mechanisms in conferring resistance to acetyl-CoA carboxylase inhibiting herbicides in a black-grass population. PLoS One 8, e69568
| Role of a novel I1781T mutation and other mechanisms in conferring resistance to acetyl-CoA carboxylase inhibiting herbicides in a black-grass population.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXht1Cmtr%2FL&md5=b66f272222d82ae21d3874752c4b6819CAS | 23936046PubMed |
Li L, Bi Y, Liu W, Yuan G, Wang J (2013) Molecular basis for resistance to fenoxaprop-p-ethyl in American sloughgrass (Beckmannia syzigachne Steud.). Pesticide Biochemistry and Physiology 105, 118–121.
| Molecular basis for resistance to fenoxaprop-p-ethyl in American sloughgrass (Beckmannia syzigachne Steud.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXit1yksb0%3D&md5=a92b4109f231435fd7cfe53628c6096eCAS |
Li L, Du L, Liu W, Yuan G, Wang J (2014) Target-site mechanism of ACCase-inhibitors resistance in American sloughgrass (Beckmannia syzigachne Steud.) from China. Pesticide Biochemistry and Physiology 110, 57–62.
| Target-site mechanism of ACCase-inhibitors resistance in American sloughgrass (Beckmannia syzigachne Steud.) from China.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXkvFSitbk%3D&md5=9ed2e3d43059a50e25056a059b8360cbCAS | 24759052PubMed |
Li LX, Liu WT, Chi YC, Guo WL, Luo XY, Wang JX (2015) Molecular mechanism of mesosulfuron-methyl resistance in multiply-resistant American sloughgrass (Beckmannia syzigachne). Weed Science 63, 781–787.
| Molecular mechanism of mesosulfuron-methyl resistance in multiply-resistant American sloughgrass (Beckmannia syzigachne).Crossref | GoogleScholarGoogle Scholar |
Mohamed IA, Li RZ, You ZG, Li ZH (2012) Japanese foxtail (Alopecurus japonicus) resistance to fenoxaprop and pinoxaden in China. Weed Science 60, 167–171.
| Japanese foxtail (Alopecurus japonicus) resistance to fenoxaprop and pinoxaden in China.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC38XmsFyntrY%3D&md5=1441bb345a0f861e7612a1a52707ec02CAS |
Pan L, Li J, Xia WW, Zhang D, Dong LY (2015a) An effective method, composed of LAMP and dCAPS, to detect different mutations in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) populations. Pesticide Biochemistry and Physiology 117, 1–8.
| An effective method, composed of LAMP and dCAPS, to detect different mutations in fenoxaprop-P-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.) populations.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXhsl2ltL7N&md5=c68d17887416baf463a7d2911fd60facCAS | 25619905PubMed |
Pan L, Li J, Zhang T, Zhang D, Dong LY (2015b) Cross-resistance patterns to acetyl coenzyme A carboxylase (ACCase) inhibitors associated with different ACCase mutations in Beckmannia syzigachne. Weed Research 55, 609–620.
| Cross-resistance patterns to acetyl coenzyme A carboxylase (ACCase) inhibitors associated with different ACCase mutations in Beckmannia syzigachne.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXhvVyru7jN&md5=1505069719631eec2e182ddbee7dc614CAS |
Pan L, Li J, Zhang W-n, Dong L (2015c) Detection of the I1781L mutation in fenoxaprop-p-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.), based on the loop-mediated isothermal amplification method. Pest Management Science 71, 123–130.
| Detection of the I1781L mutation in fenoxaprop-p-ethyl-resistant American sloughgrass (Beckmannia syzigachne Steud.), based on the loop-mediated isothermal amplification method.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXmtFSqt7g%3D&md5=9535f8e73179e7e33bc1918b88d51489CAS | 24644047PubMed |
Pan L, Gao HT, Xia WW, Zhang T, Dong LY (2016) Establishing a herbicide-metabolizing enzyme library in Beckmannia syzigachne to identify genes associated with metabolic resistance. Journal of Experimental Botany 67, 1745–1757.
| Establishing a herbicide-metabolizing enzyme library in Beckmannia syzigachne to identify genes associated with metabolic resistance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC28Xhtlant7fL&md5=28bebfc1a71fc76f5abda28a559ee0ecCAS | 26739863PubMed |
Petit C, Bay G, Pernin F, Délye C (2010) Prevalence of cross- or multiple resistance to the acetyl-coenzyme A carboxylase inhibitors fenoxaprop, clodinafop and pinoxaden in black-grass (Alopecurus myosuroides Huds.) in France. Pest Management Science 66, 168–177.
Powles SB, Yu Q (2010) Evolution in action: plants resistant to herbicides. Annual Review of Plant Biology 61, 317–347.
| Evolution in action: plants resistant to herbicides.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXnslSjsLo%3D&md5=db088051b7441ee50d6f7079028ce9dbCAS | 20192743PubMed |
Rao N, Dong LY, Li J, Zhang HJ (2008) Influence of environmental factors on seed germination and seedling emergence of American sloughgrass (Beckmannia syzigachne). Weed Science 56, 529–533.
| Influence of environmental factors on seed germination and seedling emergence of American sloughgrass (Beckmannia syzigachne).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXotlKitL4%3D&md5=17f30783f1a035a6097995fd5c680d2fCAS |
Tang W, Zhou F, Zhang Y, Chen J (2015) Resistance of American sloughgrass (Bechmannia syzigachne) populations to ACCase-inhibiting herbicides involves three different target site mutations from China. Pesticide Biochemistry and Physiology 124, 93–96.
| Resistance of American sloughgrass (Bechmannia syzigachne) populations to ACCase-inhibiting herbicides involves three different target site mutations from China.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXpsVylt70%3D&md5=9717d6f6f7c5e2193d829c66bd31a7daCAS | 26453236PubMed |
Vila-Aiub MM, Neve P, Powles SB (2009) Fitness costs associated with evolved herbicide resistance alleles in plants. New Phytologist 184, 751–767.
| Fitness costs associated with evolved herbicide resistance alleles in plants.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXhsFOls7%2FF&md5=72bfab8096d97a72651e68d5d152a95eCAS | 19825013PubMed |
Wang FH, He ZH, Sayre K, Li SD, Si JS, Feng B, Kong LG (2009) Wheat cropping systems and technologies in China. Field Crops Research 111, 181–188.
| Wheat cropping systems and technologies in China.Crossref | GoogleScholarGoogle Scholar |
Xia W, Pan L, Li J, Wang Q, Feng Y, Dong L (2015) Molecular basis of ALS- and/or ACCase-inhibitor resistance in shortawn foxtail (Alopecurus aequalis Sobol.). Pesticide Biochemistry and Physiology 122, 76–80.
| Molecular basis of ALS- and/or ACCase-inhibitor resistance in shortawn foxtail (Alopecurus aequalis Sobol.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2MXmslCntQ%3D%3D&md5=8452e9231e3652c69dbb4eef6815af11CAS | 26071810PubMed |
Xu H, Li J, Zhang D, Cheng Y, Jiang Y, Dong L (2014) Mutations at codon position 1999 of acetyl-CoA carboxylase confer resistance to ACCase-inhibiting herbicides in Japanese foxtail (Alopecurus japonicus). Pest Management Science 70, 1894–1901.
| Mutations at codon position 1999 of acetyl-CoA carboxylase confer resistance to ACCase-inhibiting herbicides in Japanese foxtail (Alopecurus japonicus).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXks1Oqt7c%3D&md5=8704b8069dffa626f49c8904c5e4fb02CAS | 24497328PubMed |
Yan SJ, Liu J (2014) Wheat herbicide application situation in China. Pesticide Market News 2, 4–6. [In Chinese]
Yu Q, Powles SB (2014) Resistance to AHAS inhibitor herbicides: current understanding. Pest Management Science 70, 1340–1350.
| Resistance to AHAS inhibitor herbicides: current understanding.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXptFKhsg%3D%3D&md5=3909e6855003175b4d7cf440388d38d4CAS | 24338926PubMed |
Yu Q, Ahmad-Hamdani MS, Han H, Christoffers MJ, Powles SB (2013) Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species. Heredity 110, 220–231.
| Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXislSisbw%3D&md5=6940e882ad05b984553efac52af233e5CAS | 23047200PubMed |
Zhang X-Q, Powles SB (2006) Six amino acid substitutions in the carboxyl-transferase domain of the plastidic acetyl-CoA carboxylase gene are linked with resistance to herbicides in a Lolium rigidum population. New Phytologist 172, 636–645.
| Six amino acid substitutions in the carboxyl-transferase domain of the plastidic acetyl-CoA carboxylase gene are linked with resistance to herbicides in a Lolium rigidum population.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhtlCjur%2FJ&md5=332b1c604031885d9477265707831c21CAS | 17096790PubMed |
