A PCR-based marker closely linked to a 2BS QTL conferring wheat yellow spot resistance for marker-assisted breeding
Fei Ren A B E , Jun Ji C , Hui Liu A B , Martin J. Barbetti A B , Kadambot H. M. Siddique B , Chunji Liu A D and Guijun Yan A B F
+ Author Affiliations
- Author Affiliations
A School of Plant Biology, Faculty of Science, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C Centre for Agricultural Resources, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences (CAS), 286 Huaizhong Road, Shijiazhuang, Hebei Province, 050021, PR China.
D CSIRO Agriculture Flagship, 306 Carmody Road, St Lucia, Qld 4067, Australia.
E School of Agricultural Science and Engineering, Shaoguan University, 288 Daxue Road, Zhenjiang District, Shaoguan, Guangdong Province, 512000, PR China.
F Corresponding author. Email: guijun.yan@uwa.edu.au
Crop and Pasture Science 67(7) 719-725 https://doi.org/10.1071/CP15312
Submitted: 6 October 2015 Accepted: 1 April 2016 Published: 28 July 2016
Abstract
Yellow spot (caused by Pyrenophora tritici-repentis) is a major foliar disease in wheat (Triticum aestivum) that has become more serious in recent years, possibly because of climate change. A major quantitative trait locus (QTL) located on the short arm of wheat chromosome 2B explaining 30–40% of the phenotypic variance has been identified as responsible for resistance to Australian yellow spot isolates, which reportedly produce mostly the ToxA effector. The closest marker linked to this QTL was a DArT marker not easy to use in large-scale selections, whereas the closest PCR-based marker available (2.7 cM) was too far away for reliably tagging the locus in wheat breeding. We therefore undertook studies to develop more closely linked and user-friendly markers for this major QTL. Forty-one new markers either synthesised from DArT markers or identified from the GrainGene database were assessed. From these, we developed a new PCR-based marker (Rfsts1), located 0.3 cM away from the major QTL. This is the first suitable marker for marker-assisted selection for yellow spot resistance in Australian wheat-breeding programs.
Additional keywords: 2BS, MAS, molecular marker, QTL, wheat, yellow spot.
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