Antifungal activity of plant extracts against Embellisia astragali, the fungal causal agent of yellow dwarf and root-rot disease of standing milkvetch
Cuiyun Zeng A B , Xiaoyu Zhu B , Zhen Cui A and Yanzhong Li A C
+ Author Affiliations
- Author Affiliations
A College of Pastoral Agriculture Science and Technology, Gansu Grassland Ecological Research Institute, State Key Laboratory of Grassland Farming Systems, Lanzhou University, PO Box 61, Lanzhou 730020, China.
B Gansu College of Traditional Chinese Medicine, Lanzhou 730000, China.
C Corresponding author. Email: liyzh@lzu.edu.cn
Crop and Pasture Science 66(7) 735-739 https://doi.org/10.1071/CP15012
Submitted: 15 January 2015 Accepted: 10 February 2015 Published: 24 June 2015
Abstract
In an attempt to find biological fungicides, we screened 18 medicinal plants used in Chinese traditional medicine for their activity against Embellisia astragali, the causative fungus of yellow dwarf and root-rot disease in standing milkvetch (Astragalus adsurgens Pall.). The antifungal efficacy of ethanolic extracts of these plants was tested in vitro. Our results show that among the 18 plants tested, the ethanolic extracts of Saposhnikovia divaricata, Allium sativum and Juglans regia totally inhibited mycelial growth of E. astragali. These three extracts also significantly inhibited spore germination of E. astragali, with inhibition rates ranging from 86% to 88%. In addition, the same extract from the same plant part indicated the strongest antifungal activity against E. astragali, with a minimal inhibitory concentration value of 50 mg mL–1. These results demonstrate that plant-derived products have a high potential to control yellow dwarf and root-rot disease in standing milkvetch.
Additional keywords: antifungal activity, medicinal plant extracts, pathogen.
References
Ameziane N, Boubaker H, Boudyach H, Msanda F, Jilal A, Benaoumar AA (2007) Antifungal activity of Moroccan plants against citrus fruit pathogens. Agronomy for Sustainable Development 27, 273–277.| Antifungal activity of Moroccan plants against citrus fruit pathogens.Crossref | GoogleScholarGoogle Scholar |
Banso A, Adeyemo S, Jeremiah P (1999) Antimicrobial properties of Vernonia amygdalina extract. Journal of Applied Science and Management 3, 9–11.
Bansod S, Rai M (2008) Antifungal activity of essential oils from Indian medicinal plants against human pathogenic Aspergillus fumigatus and A. niger. World Journal of Medical Sciences 3, 81–88.
Bovin B (1967) Crataegus. Flora of the Prairie Provinces. Provancheria 2, 51–52.
Ciccia G, Coussio J, Mongelli E (2000) Insecticidal activity against Aedes aegypti larvae of some medicinal South American plants. Journal of Ethnopharmacology 72, 185–189.
| Insecticidal activity against Aedes aegypti larvae of some medicinal South American plants.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M7gt1Gmtg%3D%3D&md5=fed536e5bb4fcd920aa1b3f07c55162bCAS | 10967471PubMed |
Davicino R, Mattar M, Casali Y, Correa S, Pettenati E, Micalizzi B (2007) Antifungal activity of plant extracts used in folk medicine in Argentina. Revista Peruana de Biología 14, 247–251.
Grover RK, Moore MJ (1962) Toxicometric studies of fungicides against brown rot organisms Sclerotinia fructicola and S. laxa. Phytopathology 52, 876–880.
Jeyaseelan EC, Pathmanathan MK, Jeyadevan JP (2010) Inhibitory effect of different solvent extracts of Vitex negundo L. and Allium sativum L. on phytopathogenic bacteria. Archives of Applied Science Research 2, 325–331.
Katole SB, Kumar P, Patil RD (2013) Environmental pollutants and livestock health: a review. Veterinary Research International 1, 1–13.
Kumar VP, Chauhan NS, Padh H, Rajani M (2006) Search for antibacterial and antifungal agents from selected Indian medicinal plants. Journal of Ethnopharmacology 107, 182–188.
| Search for antibacterial and antifungal agents from selected Indian medicinal plants.Crossref | GoogleScholarGoogle Scholar | 16678369PubMed |
Lang BJ, Pecinovsky KT (2013) Preliminary research results on the use of foliar fungicides in alfalfa production. Animal Industry Report 659, 38
Li Y, Nan Z (2007) A new species, Embellisia astragali sp. nov., causing standing milk-vetch disease in China. Mycologia 99, 406–411.
| A new species, Embellisia astragali sp. nov., causing standing milk-vetch disease in China.Crossref | GoogleScholarGoogle Scholar | 17883032PubMed |
Li YZ, Nan ZB, Hou FJ (2007) The roles of an Embellisia sp. causing yellow stunt and root rot of Astragalus adsurgens and other fungi in the decline of legume pastures in northern China. Australasian Plant Pathology 36, 397–402.
| The roles of an Embellisia sp. causing yellow stunt and root rot of Astragalus adsurgens and other fungi in the decline of legume pastures in northern China.Crossref | GoogleScholarGoogle Scholar |
Lumpkin TA, Konovsky JC, Larson KJ, McClary DC (1993) Potential new specialty crops from Asia: Azuki bean, edamame soybean, and astragalus. In ‘New crops’. pp. 45–51. (Wiley: New York)
Mahapatra SS, Sahoo BK (2013) Management of root rot disease of forage oat in Odisha. Journal of Plant Protection and Environment 10, 49–53.
Muanza D, Kim B, Euler K, Williams L (1994) Antibacterial and antifungal activities of nine medicinal plants from Zaire. Pharmaceutical Biology 32, 337–345.
| Antibacterial and antifungal activities of nine medicinal plants from Zaire.Crossref | GoogleScholarGoogle Scholar |
Nair MG, Safir GR, Siqueira JO (1991) Isolation and identification of vesicular-arbuscular mycorrhiza-stimulatory compounds from clover (Trifolium repens) roots. Applied and Environmental Microbiology 57, 434–439.
Nan Z (2000) Establishing sustainable management system for diseases of pasture crops in China. Acta Prataculturae Sinica 9, 1–9. [In Chinese]
Nyarko HD, Akpaka R (2011) A comparison of the antifungal properties of onion (Allium cepa), ginger (Zingiber officinale) and garlic (Allium sativum) against Aspergillus flavus, Aspergillus niger and Cladosporium herbarum. Research Journal of Medicinal Plant 281–287.
Oliveira I, Sousa A, Ferreira IC, Bento A, Estevinho L, Pereira JA (2008) Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food and Chemical Toxicology 46, 2326–2331.
| Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXmvVKqtLk%3D&md5=420bb0d8e3ae29226a98acb69c2b4193CAS | 18448225PubMed |
Pavela R (2004) Insecticidal activity of certain medicinal plants. Fitoterapia 75, 745–749.
| Insecticidal activity of certain medicinal plants.Crossref | GoogleScholarGoogle Scholar | 15567255PubMed |
Phongpaichit S, Subhadhirasakul S, Wattanapiromsakul C (2005) Antifungal activities of extracts from Thai medicinal plants against opportunistic fungal pathogens associated with AIDS patients. Mycoses 48, 333–338.
| Antifungal activities of extracts from Thai medicinal plants against opportunistic fungal pathogens associated with AIDS patients.Crossref | GoogleScholarGoogle Scholar | 16115104PubMed |
Quiroga EN, Sampietro AR, Vattuone MA (2001) Screening antifungal activities of selected medicinal plants. Journal of Ethnopharmacology 74, 89–96.
| Screening antifungal activities of selected medicinal plants.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DC%2BD3M7ht1Ortw%3D%3D&md5=0b41d80ca8f37091bff5e5b19a5d01e3CAS | 11137353PubMed |
Ríos JL, Recio MC (2005) Medicinal plants and antimicrobial activity. Journal of Ethnopharmacology 100, 80–84.
| Medicinal plants and antimicrobial activity.Crossref | GoogleScholarGoogle Scholar | 15964727PubMed |
Ruch BW (2001) Processing of neem for plant protection simple and sophisticated standardized extracts. In ‘Workshop on Neem and Pheromones’. 29–30 March 2001, University of Uberaba, Brazil. p. 499.
Saito H (2000) Regulation of herbal medicines in Japan. Pharmacological Research 41, 515–519.
| Regulation of herbal medicines in Japan.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3cXit1Cktrw%3D&md5=71d4e912875040af40516fc4bd4bfdffCAS | 10753549PubMed |
Shahi SK, Shukla A, Bajaj A, Medgely G, Dikshit A (1999) Broad spectrum antimycotic drug for the control of fungal infection in human beings. Current Science 76, 836–839.
Vlietinck AJ, Van Hoof L, Totte J, Lasure A, Berghe DV, Rwangabo PC, Mvukiyumwami J (1995) Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties. Journal of Ethnopharmacology 46, 31–47.
| Screening of hundred Rwandese medicinal plants for antimicrobial and antiviral properties.Crossref | GoogleScholarGoogle Scholar | 1:STN:280:DyaK287gtF2mug%3D%3D&md5=029bd15dbc65adea74892f773d2f3db0CAS | 7475121PubMed |
Wiersema JH, Kirkbride JH Jr, Gunn CR (1990) Legume (Fabaceae) nomenclature in the USDA Germplasm System. Technical Bulletin, United States Department of Agriculture, Washington, DC.
Yoon M-Y, Cha B, Kim J-C (2013) Recent trends in studies on botanical fungicides in agriculture. The Plant Pathology Journal 29, 1–9.
| Recent trends in studies on botanical fungicides in agriculture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3sXhslGhsL3O&md5=86c088e3a4d77907bc703dec9aa4f277CAS | 25288923PubMed |
Yu B, Nan Z, Li Y, Lin H (2012) Resistance of standing milkvetch (Astragalus adsurgens) varieties to Embellisia astragali. Crop & Pasture Science 63, 351–359.
| Resistance of standing milkvetch (Astragalus adsurgens) varieties to Embellisia astragali.Crossref | GoogleScholarGoogle Scholar |
Zabka M, Pavela R, Sumikova T (2012) Medicinal and culinary herbs as environmentally safe inhibitors of dangerous toxinogenic plant and human fungal pathogens. African Journal of Microbiological Research 6, 6468–6475.
| Medicinal and culinary herbs as environmentally safe inhibitors of dangerous toxinogenic plant and human fungal pathogens.Crossref | GoogleScholarGoogle Scholar |
Zhang L, Ravipati AS, Koyyalamudi SR, Jeong SC, Reddy N, Bartlett J, Smith PT, de la Cruz M, Monteiro MC, Melguizo Á (2013) Anti-fungal and anti-bacterial activities of ethanol extracts of selected traditional Chinese medicinal herbs. Asian Pacific Journal of Tropical Medicine 6, 673–681.
| Anti-fungal and anti-bacterial activities of ethanol extracts of selected traditional Chinese medicinal herbs.Crossref | GoogleScholarGoogle Scholar | 23827142PubMed |
