Development and validation of protocols for product stewardship in transgenic white clover (Trifolium repens L.): detection of the AMV CP and npt2 transgenes in seeds, herbage and hay
S. Panter A , A. Mouradov B , K. F. Smith C and G. Spangenberg A D E
+ Author Affiliations
- Author Affiliations
A Department of Environment and Primary Industries, Biosciences Research Division, AgriBio, La Trobe University, Bundoora, Vic. 3083, Australia.
B School of Applied Sciences, RMIT University, Bundoora, Vic., 3083, Australia.
C Melbourne School of Land and Environment, The University of Melbourne, Private Bag 105, Hamilton, Vic. 3300, Australia.
D Faculty of Science, Technology and Engineering, La Trobe University, Bundoora, Vic. 3083, Australia.
E Corresponding author. Email: german.spangenberg@ecodev.vic.gov.au
Crop and Pasture Science 66(10) 1039-1048 https://doi.org/10.1071/CP14337
Submitted: 1 December 2014 Accepted: 7 June 2015 Published: 8 September 2015
Abstract
White clover (Trifolium repens L.) is an important pasture legume in temperate areas throughout the world, providing fodder for grazing animals and improving soil fertility via symbiotic nitrogen fixation. However, the persistence and stress tolerance of white clover is affected by several viruses, chiefly Alfalfa mosaic virus (AMV), Clover yellow vein virus (ClYVV) and White clover mosaic virus (WClMV). Efforts to introgress natural forms of virus resistance from other Trifolium spp. into white clover and lucerne (alfalfa) have had only limited success. This has been addressed by developing white clover germplasm exhibiting viral-coat-protein-mediated resistance to AMV and non-transgenic resistance to ClYVV. This report describes PCR-based assays for detecting the transgenes associated with the H6 transformation event in seeds, fresh leaves, air-dried leaves and mixtures of air-dried herbage of white clover and perennial ryegrass (hay). Although further development is required to convert these assays for use in the field, this paper demonstrates the ability to detect these transgenes in a range of agricultural products associated with the commercial use of white clover.
Additional keywords: Alfalfa mosaic virus, GMO, transgenic, tracing, Trifolium repens, white clover.
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