Identification of fast and slow germination accessions of Brassica napus L. for genetic studies and breeding for early vigour
Jing Zhang A B , Liyong Hu A D , Bob Redden C and Guijun Yan B D
+ Author Affiliations
- Author Affiliations
A Ministry of Agriculture (MOA) Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science & Technology, Huazhong Agricultural University, Wuhan, 430070, China.
B School of Plant Biology, Faculty of Science and The UWA Institute of Agriculture, The University of Western Australia, Stirling Highway, Crawley, WA 6009, Australia.
C Australian Temperate Field Crops Collection, Victorian Department of Environment and Primary Industries, Horsham, Vic. 3401, Australia.
D Corresponding authors. Email: guijun.yan@uwa.edu.au; liyonghu@mail.hzau.edu.cn
Crop and Pasture Science 66(5) 481-491 https://doi.org/10.1071/CP14269
Submitted: 28 May 2014 Accepted: 26 December 2014 Published: 24 April 2015
Abstract
In this study, 137 canola (Brassica napus L.) accessions were evaluated for germination speed, which is a critical character in the plant life cycle. The accessions were grouped into three categories, fast (F), medium, and slow (S), with nine category F (7%) and 12 category S (9%) germination accessions identified and validated in repeated Petri dish and pot experiments. Although accessions in category F showed significantly faster germination and emergence than those in category S, seedling growth parameters did not differ greatly. Based on germination speed and seedling characteristics, four accessions with high early vigour and four with low early vigour were identified. Seed germination speed was not affected by seed weight and was not simply controlled by gibberellic acid and abscisic acid, but 10% smoke water significantly delayed seed germination. The identified accessions with contrasting early vigour can be used to study the genetic and molecular mechanism of seed germination and seedling development and to breed superior canola cultivars.
References
Abdul-Baki AA, Anderson JD (1973) Vigor determination in soybean seed by multiple criteria. Crop Science 13, 630–632.| Vigor determination in soybean seed by multiple criteria.Crossref | GoogleScholarGoogle Scholar |
Anzala F, Morère-Le Paven M, Fournier S, Rondeau D, Limami AM (2006) Physiological and molecular aspects of aspartate-derived amino acid metabolism during germination and post-germination growth in two maize genotypes differing in germination efficiency. Journal of Experimental Botany 57, 645–653.
| Physiological and molecular aspects of aspartate-derived amino acid metabolism during germination and post-germination growth in two maize genotypes differing in germination efficiency.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XovVCqsA%3D%3D&md5=61a78574ed593c477d376612e68fb6b6CAS | 16415333PubMed |
Below FE, Cazetta JO, Seebauer JR (2000) ‘Carbon/nitrogen interactions during ear and kernel development of maize.’ (Crop Science Society of America: Madison, WI, USA)
Bettey M, Finch-Savage WE, King GJ, Lynn JR (2000) Quantitative genetic analysis of seed vigour and pre-emergence seedling growth traits in Brassica oleracea. New Phytologist 148, 277–286.
| Quantitative genetic analysis of seed vigour and pre-emergence seedling growth traits in Brassica oleracea.Crossref | GoogleScholarGoogle Scholar |
Bewley JD, Black M (1994) ‘Seeds, physiology of development and germination.’ (Plenum Press: New York)
Chumpookam J, Lin HL, Shiesh CC (2012) Effect of smoke-water derived from burnt dry rice straw (Oryza sativa) on seed germination and growth of papaya seedling (Carica papaya) Cultivar “Tainung No. 2”. Horticultural Science 47, 741–744.
Cisse N, Gebisa E (2003) Genetic variation and relationships among seedling vigor traits in sorghum. Crop Science 43, 824–828.
| Genetic variation and relationships among seedling vigor traits in sorghum.Crossref | GoogleScholarGoogle Scholar |
Coolbear P, Fancis A, Grierson D (1984) The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds. Journal of Experimental Botany 35, 1609–1617.
| The effect of low temperature pre-sowing treatment on the germination performance and membrane integrity of artificially aged tomato seeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2MXitFentQ%3D%3D&md5=5ac44cac95010793d36d4fdd788c1d14CAS |
Cui KH, Peng SB, Xing YZ, Xu CG, Yu SB, Zhang Q (2002) Molecular dissection of seedling-vigor and associated physiological traits in rice. Theoretical and Applied Genetics 105, 745–753.
| Molecular dissection of seedling-vigor and associated physiological traits in rice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38XnvVSgs78%3D&md5=f28646817f015c316cbf64848a0282f5CAS |
Dang X, Thi TGT, Dong G, Wang H, Edzesi WM, Hong D (2014) Genetic diversity and association mapping of seed vigor in rice (Oryza sativa L.). Planta 239, 1309–1319.
| Genetic diversity and association mapping of seed vigor in rice (Oryza sativa L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXkvVGrsLg%3D&md5=cdfc2827f46fec6ad0f197ab0edf4e50CAS | 24668487PubMed |
Daws MI, Burslem DFRP, Crabtree LM, Kirkman P, Mullins CE, Dalling JW (2002) Differences in seed germination responses may promote coexistence of four sympatric Piper species. Functional Ecology 16, 258–267.
| Differences in seed germination responses may promote coexistence of four sympatric Piper species.Crossref | GoogleScholarGoogle Scholar |
Daws MI, Davies J, Pritchard HW, Brown NAC, van Staden J (2007) Butenolide from plant-derived smoke enhances germination and seedling growth of arable weed species. Plant Growth Regulation 51, 73–82.
| Butenolide from plant-derived smoke enhances germination and seedling growth of arable weed species.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXlslWmuw%3D%3D&md5=0922ef4d2e774f52901dad0bc8aeaa50CAS |
Doherty LC, Cohn MA (2000) Seed dormancy in red rice (Oryza sativa). XI. Commercial liquid smoke elicits germination. Seed Science Research 10, 415–421.
Einhelling FA, Schon MK, Rasmussen JA (1982) Synergistic effects of four cinnamic acid compounds on grain sorghum. Journal of Plant Growth Regulation 1, 251–258.
Farooq M, Basra SMA, Afzal I, Khaliq A (2006) Optimization of hydropriming techniques for rice seed invigoration. Seed Science and Technology 34, 507–512.
| Optimization of hydropriming techniques for rice seed invigoration.Crossref | GoogleScholarGoogle Scholar |
Finch-Savage WE, Clay HA, Lynn JR, Morris K (2010) Towards a genetic understanding of seed vigour in small-seeded crops using natural variation in Brassica oleracea. Plant Science 179, 582–589.
| Towards a genetic understanding of seed vigour in small-seeded crops using natural variation in Brassica oleracea.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlKht7jI&md5=90fc1cd70d709229d4632384d3100371CAS |
Flematti GR, Ghisalberti EL, Dixon KW, Trengove RD (2004) A compound from smoke that promotes seed germination. Science 305, 977
| A compound from smoke that promotes seed germination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXmslWqtrs%3D&md5=0837651fe1de5c1a9cbc59bc2765e6b1CAS | 15247439PubMed |
Hasan M, Seyis F, Badani AG, Pons-Kühnemann J, Friedt W, Lühs W, Snowdon RJ (2006) Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers. Genetic Resources and Crop Evolution 53, 793–802.
| Analysis of genetic diversity in the Brassica napus L. gene pool using SSR markers.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XmtFahsbo%3D&md5=c488a7023964cbad4dc45bff5ce2b1ecCAS |
Hegarty TW (1978) Seed bed condition and seedling establishment. Acta Horticulturae 83, 297–307.
Jain N, Staden JV (2007) The potential of the smoke-derived compound 3-methyl-2H-furo[2,3-c]pyran-2-one as a priming agent for tomato seeds. Seed Science Research 17, 175–181.
| The potential of the smoke-derived compound 3-methyl-2H-furo[2,3-c]pyran-2-one as a priming agent for tomato seeds.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXht1Srs77K&md5=a50fa7b7d127fef2080d402df97a88a5CAS |
Kawade RM, Ugale SD, Patil RB (1987) Effect of seed size on germination, seedling vigor, and test weight of pearl millet. Seed Research 15, 210–213.
Kikuzawa K, Koyama H (1999) Scaling of soil water absorption by seeds: an experiment using seed analogues. Seed Science Research 9, 171–178.
Koornneef M, Alonso-Blanco C, Vreugdenhil D (2004) Naturally occurring genetic variation in Arabidopsis thaliana. Annual Review of Plant Biology 55, 141–172.
| Naturally occurring genetic variation in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXlvFeis7s%3D&md5=e395f4da141fd7c8c8157bcc858d1ae7CAS | 15377217PubMed |
Kucera B, Cohn MA, Leubner-Metzger G (2005) Plant hormone interactions during seed dormancy release and germination. Seed Science Research 15, 281–307.
| Plant hormone interactions during seed dormancy release and germination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XhsFGrtr0%3D&md5=95228a6231ab8e29728e52e014d790ebCAS |
Lafond GP, Baker RJ (1986) Effects of genotype and seed size on speed of emergence and seedling vigor in nine spring wheat cultivars. Crop Science 26, 341–346.
| Effects of genotype and seed size on speed of emergence and seedling vigor in nine spring wheat cultivars.Crossref | GoogleScholarGoogle Scholar |
Light ME, Burger BV, Staerk D, Kohout L, Staden JV (2010) Butenolides from plant-derived smoke: natural plant-growth regulators with antagonistic actions on seed germination. Journal of Natural Products 73, 267–269.
| Butenolides from plant-derived smoke: natural plant-growth regulators with antagonistic actions on seed germination.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXmslWqsw%3D%3D&md5=b0d6f1f12b0f59f46a0bc32e5759aa6fCAS | 20078110PubMed |
Limami AM, Rouillon C, Glevarec G, Gallais A, Hirel B (2002) Genetic and physiological analysis of germination efficiency in maize in relation to nitrogen metabolism reveals the importance of cytosolic glutamine synthetase. Plant Physiology 130, 1860–1870.
| Genetic and physiological analysis of germination efficiency in maize in relation to nitrogen metabolism reveals the importance of cytosolic glutamine synthetase.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXktlGk&md5=ef46e0231180aa996afd27c11b438515CAS | 12481069PubMed |
Maguire JD (1962) Speed of germination—aid in selection and evaluation for seedling emergence and vigor. Crop Science 2, 176–177.
| Speed of germination—aid in selection and evaluation for seedling emergence and vigor.Crossref | GoogleScholarGoogle Scholar |
Mavi K (2010) The relationship between seed coat color and seed quality in watermelon Crimson sweet. Horticultural Science (Prague) 37, 62–69.
McKeon GM (1984) Field changes in germination requirements: effect of natural rainfall on potential germination speed and light requirement of Stylosanthes humilis, Stylosanthes hamata and Digitaria ciliaris. Australian Journal of Agricultural Research 35, 807–819.
| Field changes in germination requirements: effect of natural rainfall on potential germination speed and light requirement of Stylosanthes humilis, Stylosanthes hamata and Digitaria ciliaris.Crossref | GoogleScholarGoogle Scholar |
Mei JQ, Fu Y, Qian LW, Xu XF, Li JN, Qian W (2011) Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach. Plant Breeding 130, 333–337.
| Effectively widening the gene pool of oilseed rape (Brassica napus L.) by using Chinese B. rapa in a ‘virtual allopolyploid’ approach.Crossref | GoogleScholarGoogle Scholar |
Pet G, Garretsen F (1983) Genetical and enviromental factors influencing seed size of tomato (Lycopersicon esculentum Mill.) and the effect of seed size on growth and development of tomato plants. Euphytica 32, 711–718.
| Genetical and enviromental factors influencing seed size of tomato (Lycopersicon esculentum Mill.) and the effect of seed size on growth and development of tomato plants.Crossref | GoogleScholarGoogle Scholar |
Pinthus MJ, Kimel U (1979) Speed of germination as a criterion of seed vigor in soybeans. Crop Science 19, 291–292.
| Speed of germination as a criterion of seed vigor in soybeans.Crossref | GoogleScholarGoogle Scholar |
Pitorre D, Llauro C, Jobet E, Guilleminot J, Brizard J-P, Delseny M, Lasserre E (2010) RLK7, a leucine-rich repeat receptor-like kinase, is required for proper germination speed and tolerance to oxidative stress in Arabidopsis thaliana. Planta 232, 1339–1353.
| RLK7, a leucine-rich repeat receptor-like kinase, is required for proper germination speed and tolerance to oxidative stress in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhtlSgs7jN&md5=002c1bf09476c033eed9632c802e1661CAS | 20811905PubMed |
Roy SKS, Hamid A, Giashuddin Miah M, Hashem A (1996) Seed size variation and its effects on germination and seedling vigour in rice. Journal of Agronomy and Crop Science 176, 79–82.
| Seed size variation and its effects on germination and seedling vigour in rice.Crossref | GoogleScholarGoogle Scholar |
Saranga Y, Levi A, Horcika P, Wolf S (1998) Large sunflower seeds are characterized by low embryo vigor. Journal of the American Society for Horticultural Science 123, 470–474.
Schmuths H, Bachmann K, Weer WE, Horres R, Hoffmann MH (2006) Effects of preconditioning and temperature during germination of 73 natural accessions of Arabidopsis thaliana. Annals of Botany 97, 623–634.
| Effects of preconditioning and temperature during germination of 73 natural accessions of Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 16464878PubMed |
Schopfer P, Plachy C (1984) Control of Seed germination by abscisic acid. II. Effect on embryo water uptake in Brassica napus L. Plant Physiology 76, 155–160.
Wang M, Liu M, Li D, Wu J, Li X, Yang Y (2010) Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus. Plant Cell, Tissue and Organ Culture 102, 205–211.
| Overexpression of FAD2 promotes seed germination and hypocotyl elongation in Brassica napus.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXnvVejtL8%3D&md5=45f18e729da0afb0be26b6f56804955aCAS |
Wang Y, Hecker AG, Hauser BA (2014) The APX4 locus regulates seed vigor and seedling growth in Arabidopsis thaliana. Planta 239, 909–919.
| The APX4 locus regulates seed vigor and seedling growth in Arabidopsis thaliana.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXmtVWhug%3D%3D&md5=5af53f1c95d869a2537306c53f597cabCAS | 24407512PubMed |
Willenborg CJ, Wildeman JC, Miller AK, Rossnagel BG, Shirtliffe SJ (2005) Oat germination characteristics differ among genotypes, seed sizes, and osmotic potentials. Crop Science 45, 2023–2029.
| Oat germination characteristics differ among genotypes, seed sizes, and osmotic potentials.Crossref | GoogleScholarGoogle Scholar |
Xie L, Tan Z, Zhou Y, Xu R, Feng L, Xing Y, Qi X (2014) Identification and fine mapping of quantitative trait loci for seed vigor in germination and seedling establishment in rice. Journal of Integrative Plant Biology 56, 749–759.
| Identification and fine mapping of quantitative trait loci for seed vigor in germination and seedling establishment in rice.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC2cXht1egtrjN&md5=dccda4412948506f1bae52491f21aa86CAS | 24571491PubMed |
Yamauchi M, Winn T (1996) Rice seed vigor and seedling establishment in anaerobic soil. Crop Science 36, 680–686.
| Rice seed vigor and seedling establishment in anaerobic soil.Crossref | GoogleScholarGoogle Scholar |
Zhang W, Gusta LV (2010) Germination response of black and yellow seed coated canola (Brassica napus) lines to chemical treatments under cold temperature conditions. Plant Growth Regulation 60, 105–114.
| Germination response of black and yellow seed coated canola (Brassica napus) lines to chemical treatments under cold temperature conditions.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXht12htbk%3D&md5=7458fc2d6fa10a7643efe49be7ed7e0dCAS |
Zhang J, Cui Y, Zhang L, Wang Y, Li J, Yan G, Hu L (2013) Seed coat color determines seed germination, seedling growth and seed composition of canola (Brassica napus). International Journal of Agriculture and Biology 15, 535–540.
