1Sl(1B) Chromosome substitution in Chinese Spring wheat promotes starch granule development and starch biosynthesis
Min Cao A , Guanxing Chen A , Chang Wang A , Shoumin Zhen A , Xiaohui Li A , Wenying Zhang B , F. J. Zeller C , S. L. K. Hsam C , Yingkao Hu A D and Yueming Yan A B D
+ Author Affiliations
- Author Affiliations
A College of Life Science, Capital Normal University, Beijing 100048, China.
B Hubei Collaborative Innovation Center for Grain Industry (HCICGI), Yangze University, Jingzhou 434025, China.
C Division of Plant Breeding and Applied Genetics, Technical University of Munich, D-85354, Freising-Weihenstephan, Germany.
D Corresponding authors. Email: yingkaohu@yahoo.com; yanym@cnu.edu.cn
Crop and Pasture Science 66(9) 894-903 https://doi.org/10.1071/CP14256
Submitted: 6 September 2014 Accepted: 27 April 2015 Published: 4 September 2015
Abstract
The common wheat variety Chinese Spring (CS) chromosome substitution line CS-1Sl(1B) was used in this study, in which the 1B chromosome in CS (Triticum aestivum L., 2n = 6x = 42, AABBDD) was substituted by the 1Sl from Aegilops longissima (2n = 2x = 14, SlSl). The results showed that the substitution of 1B in CS by 1Sl chromosome could significantly increase amylopectin and total starch contents. The dynamic changes in starch granules during grain development in CS and CS-1Sl(1B) demonstrated that the substitution line possessed higher amount of A-type starch granules and greater diameter of both A- and B-granules. qRT-PCR revealed that some key genes involved in starch biosynthesis, such as starch synthases (SSI, SSII and SSIII), starch branching enzymes (SBE IIa and SBE IIb) and granule-bound starch synthase (GBSS I), displayed higher transcript levels of mRNA expressions during grain development in CS-1Sl(1B). Our results indicate that the substituted 1Sl chromosome carries important genes that influence starch granule development and starch biosynthesis, which may be used as potential gene resources for improvement of wheat starch quality.
Additional keywords: qRT-PCR, 1Sl genome, starch granules, starch biosynthesis, wheat substitution line.
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