Detection of molecular markers associated with yield and yield components in durum wheat (Triticum turgidum L. var. durum) under saline conditions
S. Dura A E , M. Duwayri B , M. Nachit C and F. Al Sheyab D
+ Author Affiliations
- Author Affiliations
A National Center for Agricultural Research and Extension, Biotechnology Department, Baqa’, Jordan.
B University of Jordan, Faculty of Agriculture, Department of Horticulture and Crop Science, Amman, Jordan.
C Biodiversity and Integrated Gene Management, ICARDA, PO Box 6299, Rabat-Instituts, Morocco.
D Jordan University of Science and Technology, Irbid, Jordan.
E Corresponding author. Email: susanduraa@yahoo.com
Crop and Pasture Science 64(10) 957-964 https://doi.org/10.1071/CP13287
Submitted: 20 August 2013 Accepted: 12 November 2013 Published: 13 December 2013
Abstract
Durum wheat is one of the most important staple food crops, grown mainly in the Mediterranean region where its productivity is drastically affected by salinity. The objective of this study was to identify markers associated with grain yield and its related traits under saline conditions. A population of 114 F8 recombinant inbred lines (RILs) was derived by single-seed descent from a cross between Belikh2 (salinity-tolerant variety) and Omrabi5 (less salinity tolerant) was grown under non-saline and saline conditions in a glasshouse. Phenotypic data of the RILs and parental lines were measured for 15 agronomic traits. Association of 96 simple sequence repeat (SSR) loci covering all 14 chromosomes with 15 agronomic traits was analysed with a mixed linear model. In total, 49 SSR loci were significantly associated with these traits. Under saline conditions, 12 markers were associated with phenological traits and 19 markers were associated with yield and yield components. Marker alleles from Belikh2 were associated with a positive effect for the majority of markers associated with yield and yield components. Under saline condition, five markers (Xwmc182, Xwmc388, Xwmc398, Xbarc61, and Xwmc177) were closely linked with grain yield, located on chromosomes 2A, 3A, 3B, 4B, 5A, 6B, and 7A. These markers could be used for marker-assisted selection in durum wheat breeding under saline conditions.
Additional keywords: association mapping, durum wheat, marker-assisted selection, salinity tolerance, SSR.
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