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RESEARCH ARTICLE

Development of a TILLING resource in durum wheat for reverse- and forward-genetic analyses

R. Bovina A , A. Brunazzi A , G. Gasparini A , F. Sestili B , S. Palombieri B , E. Botticella B , D. Lafiandra B , P. Mantovani A and A. Massi A C
+ Author Affiliations
- Author Affiliations

A Società Produttori Sementi S.p.A., Via Macero 1, 40050 Argelato, Bologna, Italy.

B Department of Science and Technology for Agriculture, Forest, Nature and Energy (DAFNE), Tuscia University, Via San Camillo de Lellis s.n.c., 01100 Viterbo, Italy.

C Corresponding author. Email: a.massi@prosementi.com

Crop and Pasture Science 65(1) 112-124 https://doi.org/10.1071/CP13226
Submitted: 28 June 2013  Accepted: 20 October 2013   Published: 13 January 2014

Abstract

A durum wheat TILLING (targeting induced local lesions in genomes) population of 2601 M3 families was developed from cv. Svevo using ethyl methanesulfonate as a chemical mutagen. The entire M3 population was field-grown for phenotypic evaluations. Despite the polyploid nature of the wheat genome, a preliminarily phenotypic screening showed a high frequency of morphological alterations (~22%); specific phenotyping for seed morphology was undertaken. Furthermore, a reverse-genetics experiment was performed on DNA collected from M2 leaves for the homoeologous genes SBEIIa-A and SBEIIa-B involved in starch metabolism. One non-sense mutation for both genes was identified; specific crosses are planned in order to pyramid the two mutations.

Additional keywords: tilling, forward-genetics, reverse-genetics, Triticum durum, Svevo.


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