Genetic and environment interactions of seed storage proteins in narrow-leafed lupin (Lupinus angustifolius)
Shahidul Islam A D E , Wujun Ma B C , Bevan J. Buirchell B , Rudi Appels C and Guijun Yan A
+ Author Affiliations
- Author Affiliations
A School of Plant Biology, Faculty of Science and The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
C Centre for Comparative Genomics, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
D Department of Horticulture, Bangladesh Agricultural University, Mymensingh – 2202, Bangladesh.
E Corresponding author. Email: islamm06@student.uwa.edu.au
Crop and Pasture Science 63(12) 1066-1074 https://doi.org/10.1071/CP12268
Submitted: 23 July 2012 Accepted: 25 December 2012 Published: 4 March 2013
Abstract
This study investigated the genetic and environmental influences and their interactions on seed protein profiles of five narrow-leafed lupin cultivars grown under three different environmental conditions. High throughput MALDI-TOF mass spectrometry revealed 133 reproducible seed protein mass peaks. Thirty-one seed protein mass peaks were detected in all 15 combinations of cultivar × environment. Twenty mass peaks were influenced by cultivars irrespective of environment. Only six protein mass peaks were influenced by environments. Seventy-six mass peaks were highly variable. Number of mass peaks of lupin seed protein is mostly genetically controlled (P = 0.008) with no significant influence of the environment (P = 0.131). Environment and cultivar interactions were not significant (P = 0.889). Multivariate analyses of mass peak profiles supported the above analysis showing that protein mass peak profile was significantly (P = 0.001) influenced by cultivar but not by environment (P = 0.053). This result indicates the possibility of breeding new lupin cultivars targeting specific proteins for human food and animal feed without being too concerned about environmental influences.
Additional keywords: cultivar, MALDI-TOF, mass peak profile, seed protein.
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