Molecular mapping and validation of Rlm1 gene for resistance to Leptosphaeria maculans in canola (Brassica napus L.)
Rosy Raman A D , Belinda Taylor A , Kurt Lindbeck A , Neil Coombes A , Denise Barbulescu B , Phil Salisbury B C and Harsh Raman A D E
+ Author Affiliations
- Author Affiliations
A EH Graham Centre for Agricultural Innovation (an alliance between NSW Department of Primary Industries and Charles Sturt University), Wagga Wagga Agricultural Institute, PMB, Wagga Wagga, NSW 2650, Australia.
B Department of Primary Industries, Private Bag 260, Horsham, Vic. 3401, Australia.
C Department of Agriculture and Food Systems, Melbourne School of Land and Environment, The University of Melbourne, Vic. 3010, Australia.
D NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, PMB, Wagga Wagga, NSW 2650, Australia.
E Corresponding author. Email: harsh.raman@dpi.nsw.gov.au
Crop and Pasture Science 63(10) 1007-1017 https://doi.org/10.1071/CP12255
Submitted: 12 July 2012 Accepted: 23 October 2012 Published: 18 December 2012
Abstract
European winter canola (Brassica napus L.) cultivars harbour genes for durable resistance to the fungus Leptosphaeria maculans, which causes blackleg disease under Australian environmental conditions. Previous studies have shown that resistance in winter-type cultivars Maxol and Columbus is controlled by two genes, Rlm1 and Rlm3, which have been mapped using randomly amplified polymorphic DNA markers onto chromosome A7. We mapped a doubled-haploid population that consisted of 101 lines from a cross between Maxol*1 and Westar-10 using diversity arrays technology and simple sequence repeat (SSR)-based markers. Two SSR marker loci, Xol12-e03 and Xra2-a05b, flanked the Rlm1 locus at an interval of 6.7 cM, which corresponds to ~3.2 Mb of the Brassica rapa genomic sequence; this region contains several genes encoding putative kinase and leucine-rich repeat-type disease-resistance proteins. SSR markers were further tested for their linkage with the Rlm1 locus in an independent population derived from Columbus*3/Westar-10. Our results showed that SSR markers linked to Rlm1 can be useful for monitoring Rlm1 gene introgression in breeding populations derived from Maxol and Columbus.
Additional keywords: blackleg, marker-assisted selection, Rlm1, Rlm3, race specific resistance genes, simple sequence repeat markers.
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