Selection and characterisation of sugarcane mutants with improved resistance to brown rust obtained by induced mutation
María I. Oloriz A C E , Víctor Gil B E , Luis Rojas A , Novisel Veitía A , Monica Höfte C D and Elio Jiménez A D
+ Author Affiliations
- Author Affiliations
A Instituto de Biotecnología de las Plantas, Universidad Central ‘Marta Abreu’ de Las Villas, Carretera a Camajuaní Km. 5½, Santa Clara, Villa Clara, Cuba, CP 54830.
B Centro de Investigaciones Agropecuarias, Universidad Central ‘Marta Abreu’ de Las Villas, Carretera a Camajuaní Km. 5½, Santa Clara, Villa Clara, Cuba, CP 54830.
C Department Crop Protection, Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
D Corresponding authors: Emails: monica.hofte@ugent.be, ejimenez@ibp.co.cu
E María I. Oloriz and Víctor Gil contributed equally to this work.
Crop and Pasture Science 62(12) 1037-1044 https://doi.org/10.1071/CP11180
Submitted: 14 July 2011 Accepted: 18 January 2012 Published: 10 February 2012
Abstract
The brown rust susceptible sugarcane genotype B4362 was subjected to in vitro tissue culture and physical and chemical mutation induction procedures. Five brown rust resistant mutants with hypersensitive response to Puccinia melanocephala were selected out of a total population of 11 167 regenerated plants. High selection frequency was obtained with both mutagenic treatments, although chemical mutagenesis (NaN3) resulted in higher selection frequencies for brown rust resistance than gamma irradiation (60Co). The brown rust resistant mutants showed variations in molecular, morphological, and agronomic traits. Traits such as internode shape, bud shape, leaf sheath hairiness, outer auricule shape, intensity of flowering, stool growth habit, number of stalks per stool, and smut susceptibility were modified in brown rust resistant mutants. In addition, sugar yield was improved in two mutants with increments in stalk length, stalk number, and stalk diameter. Mutation induction proved to be suitable for the generation of new sources of brown rust resistance in sugarcane.
Additional keywords: branching, chemical mutagens, disease resistance, erect growth, flowering, gamma irradiation, hypersensitive response, Puccinia melanocephala, Saccharum, smut.
References
Ali A, Naz S, Alam SS, Iqbal J (2007) In vitro induced mutation for screening of red rot (Colletotrichum falcatum) resistance in sugarcane (Saccharum officinarum). Pakistan Journal of Botany 39, 1979–1994.Arro JA (2005) Genetic diversity among sugarcane clones using Target Region Amplification Polymorphism (TRAP) markers and pedigree relationships. MSc Dissertation, Louisiana State University, Baton Rouge, LA, USA. http://etd.lsu.edu/docs/available/etd-01202005-134859/unrestricted/Arro_thesis.pdf
Arruda P, Silva TR (2007) Transcriptome analysis of the sugarcane genome for crop improvement. In ‘Genomics assisted crop improvement. Vol. 2: Genomics applications in crops’. (Eds RK Varshney, R Tuberosa) pp. 483–494. (Springer: Dordrecht, Netherlands)
Bai D, Knott KR (1992) Suppression of rust resistance in bread wheat (Triticum aestivum L.) by D-genome chromosomes. Genome 35, 276–282.
| Suppression of rust resistance in bread wheat (Triticum aestivum L.) by D-genome chromosomes.Crossref | GoogleScholarGoogle Scholar |
Bernard FA (1980) Considerations of the appearance of sugarcane rust disease in the Dominican Republic. Proceedings International Society of Sugar Cane Technologists 17, 1382–1386.
Chinea A (1997) Evaluación fitopatológica del germoplasma de la caña de azúcar. In ‘Recursos genéticos de la caña de azúcar’. (Ed. F Valdès) pp. 37–47. (IMAGO: Ciudad de la Habana)
Chintamanani S, Hulbert SH, Johal SG, Balint-Kurti PJ (2010) Identification of a maize locus that modulates the hypersensitive defense response, using mutant-assisted gene identification and characterization. Genetics 184, 813–825.
| Identification of a maize locus that modulates the hypersensitive defense response, using mutant-assisted gene identification and characterization.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BC3cXhsFOnurvP&md5=33d8eb366ee17e055793ccd8e759a849CAS |
Comstock JC, Milligan SB (2007) Comparison of CL and CP germplasm reactions to Sugarcane yellow leaf virus, Leifsonia xyli subsp. xyli and Puccinia melanocephala. Journal of the American Society of Sugar Cane Technologists 27, 79–88.
Comstock JC, Shine J (1992) Effect of rust on sugarcane growth and biomass. Plant Disease 76, 175–177.
| Effect of rust on sugarcane growth and biomass.Crossref | GoogleScholarGoogle Scholar |
Comstock JC, Wu KK, Schnell RJ (1992b) Heritability of resistance to sugar cane rust. Sugar Cane 6, 7–10.
Daugrois JH, Grivet L, Roques D, Hoarau JY, Lombard H, Glaszm Ann JC, D’Hont A (1996) A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar ‘R570’. Theoretical and Applied Genetics 92, 1059–1064.
| A putative major gene for rust resistance linked with a RFLP marker in sugarcane cultivar ‘R570’.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XltFOnurk%3D&md5=33e32458774a9feea54dc11723ae5e18CAS |
Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Molecular Biology Reporter 1, 19–21.
| A plant DNA minipreparation: version II.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXksFWhtrk%3D&md5=5da0719dc309d1dcca4053b589c30ff6CAS |
Evans MM, Poethig RS (1995) Gibberellins promote vegetative phase change and reproductive maturity in maize. Plant Physiology 108, 475–487.
| Gibberellins promote vegetative phase change and reproductive maturity in maize.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXmtlarurc%3D&md5=4c5b166d85d104495e01c3dd0b85960dCAS |
Ferguson BJ, Beveridge CA (2009) Roles for auxin, cytokinin, and strigolactone in regulating shoot branching. Plant Physiology 149, 1929–1944.
| Roles for auxin, cytokinin, and strigolactone in regulating shoot branching.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1MXks1Wnsr4%3D&md5=7adc14834c0d372bec4cb5d9947a92edCAS |
Hogarth DM, Ryan CC, Taylor PWJ (1993) Quantitative inheritance of rust reistance in sugar cane. Field Crops Research 34, 187–193.
| Quantitative inheritance of rust reistance in sugar cane.Crossref | GoogleScholarGoogle Scholar |
Hoy J (2007) New option for brown rust control? Sugar Journal, March 2007. www.SugarJounal.com
Hutchinson PB (1969) A note on disease resistance ratings of sugarcane varieties. Proceedings International Society of Sugar Cane Technologists 13, 1087–1089.
Jørgensen JH (1992) Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley. Euphytica 63, 141–152.
| Discovery, characterization and exploitation of Mlo powdery mildew resistance in barley.Crossref | GoogleScholarGoogle Scholar |
Kaur A, Gosal SS, Gill R, Thind KS (2001) Induction of plant regeneration and somaclonal variation for some agronomic traits in sugarcane (Saccharum officinarum L.). Crop Improvement 28, 167–172.
Khan AI, Dahot MU, Khatri A (2007) study of genetic variability in sugarcane induced through mutation breeding. Pakistan Journal of Botany 39, 1489–1501.
Knott DR (2000) Inheritance of resistance to stem rust in Medea durum wheat and the role of suppressors. Crop Science 40, 98–102.
| Inheritance of resistance to stem rust in Medea durum wheat and the role of suppressors.Crossref | GoogleScholarGoogle Scholar |
Kolmer JA (1996) Genetics of resistance to wheat leaf rust 1. Annual Review of Phytopathology 34, 435–455.
| Genetics of resistance to wheat leaf rust 1.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK28XlsFOhsLc%3D&md5=f353fa18d3b6360c64d2fab98d8cfb7fCAS |
Liu G, Zhou HK, Hu H, Zhu ZH, Hayat Y, Xu H, Yang J (2007) Genetic analysis for brix weight per stool and its component traits in sugarcane (Saccharum officinarum). Journal of Zhejiang University - Science B 8, 860–866.
| Genetic analysis for brix weight per stool and its component traits in sugarcane (Saccharum officinarum).Crossref | GoogleScholarGoogle Scholar |
McIntyre CL, Casu RE, Drenth J, Knight D, Whan VA, Croft BJ, Jordan DR, Manners JM (2005) Resistance gene analogues in sugarcane and sorghum and their association with quantitative trait loci for rust resistance. Genome 48, 391–400.
| Resistance gene analogues in sugarcane and sorghum and their association with quantitative trait loci for rust resistance.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2MXpsVaqtr4%3D&md5=aa3e6ccde809811fced8654973a2b21dCAS |
Ming R, Wang YW, Draye X, Moore PH, Irvine JE, Paterson HA (2002) Molecular dissection of complex traits in autopolyploids: mapping QTLs affecting sugar yield and related traits in sugarcane. Theoretical and Applied Genetics 105, 332–345.
| Molecular dissection of complex traits in autopolyploids: mapping QTLs affecting sugar yield and related traits in sugarcane.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xntlejsro%3D&md5=76eaae255f9a1a15d8f6a36704f4f4b5CAS |
Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiologia Plantarum 15, 473–497.
| A revised medium for rapid growth and bioassays with tobacco tissue culture.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaF3sXksFKm&md5=28d6df87bdd05974747ce998324770d9CAS |
Nelson JC, Singh RP, Autrique JE, Sorrells ME (1997) Mapping genes conferring and suppressing leaf rust resistance in wheat. Crop Science 37, 1928–1935.
| Mapping genes conferring and suppressing leaf rust resistance in wheat.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXjtlKktg%3D%3D&md5=2aa2167ba3b0a3a30ec81171373f2002CAS |
Oloriz MI, Gil V, Rojas L, Portal O, Izquierdo Y, Jiménez E, Höfte M (2012) Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling. Plant Cell Reports
| Sugarcane genes differentially expressed in response to Puccinia melanocephala infection: identification and transcript profiling.Crossref | GoogleScholarGoogle Scholar | (In press).
Olsen O, Wang X, Von Wettstein D (1993) Sodium azide mutagenesis: Preferential generation of A·T → G·C transitions in the barley Antl8 gene. Proceedings of the National Academy of Sciences of the United States of America 90, 8043–8047.
| Sodium azide mutagenesis: Preferential generation of A·T → G·C transitions in the barley Antl8 gene.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3sXmsVant7o%3D&md5=e0311fdb2f9491d8f199c0d3627379f9CAS |
Patade VY, Suprasanna P, Bapat VA, Kulkarni UG (2006) Selection for abiotic salinity and drought stress tolerance and molecular characterization of tolerant lines in sugarcane. BARC Newsletter 273, 244–257.
Pavan S, Jacobsen E, Visser R, Bai Y (2010) Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance. Molecular Breeding 25, 1–12.
| Loss of susceptibility as a novel breeding strategy for durable and broad-spectrum resistance.Crossref | GoogleScholarGoogle Scholar |
Pérez JN, Jiménez E, Gómez R (1998) Field performance of selected sugarcane (Sacharum spp. hybrids) mutants. In ‘Somaclonal variation and induced mutations in crop improvement’. (Eds S Mohan, DS Brar, S Ahloowalia) pp. 447–462. (Kluwer Academic Publisher: Dordrecht, the Netherlands)
Purdy LH, Liu LJ, Dean JL (1983) Sugarcane rust: A newly important disease. Plant Disease 67, 1292–1295.
| Sugarcane rust: A newly important disease.Crossref | GoogleScholarGoogle Scholar |
Raboin LM, Selvi A, Nibouche S, Miranda Oliveira K, Pauquet J, Calatayud C, Zapater M, Garsmeur O, Telismart H, Dintinger J, Hoarau JH, Costet L, Carlier J, D’Hont A (2006) Genetic of sugarcane resistance to smut (Ustilago scitaminea): characterisation of pathogen diversity, QTL mapping in a bi-parental progeny and associations study among modern cultivars. In ‘Vth ISSCT Molecular Biology Workshop’. Réduit, Mauritius, 3–7 April 2006. http://issct.intnet.mu/past-workshops/molbiolabstract06.html#b
Raboin LM, Oliveira KM, Lecunff L, Telismart H, Roques D, Butterfield M, Hoarau JY, D’Hont A (2006b) Genetic mapping in sugarcane, a high polyploid, using bi-parental progeny: identification of a gene controlling stalk colour and a new rust resistance gene. Theoretical and Applied Genetics 112, 1382–1391.
| Genetic mapping in sugarcane, a high polyploid, using bi-parental progeny: identification of a gene controlling stalk colour and a new rust resistance gene.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD28XjtFamtb4%3D&md5=e99930ef19d1a484d80c9a0b5a2800d7CAS |
Raboin LM, Pauquet J, Butterfield M, D’Hont A, Glaszmann JC (2008) Analysis of genome-wide linkage disequilibrium in the highly polyploid sugarcane. Theoretical and Applied Genetics 116, 701–714.
| Analysis of genome-wide linkage disequilibrium in the highly polyploid sugarcane.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXisFCqtb4%3D&md5=9b78bef0c67059c943ee5d631ad68e01CAS |
Raid RN, Comstock JC (2006) Sugarcane rust disease. In ‘Florida sugarcane handbook’. (Ed. RA Gilbert) University of Florida Extension IFAS. Available at: http://edis.ifas.ufl.edu/SC007
Ramdoyal K, Sullivan S, Lim Shin Chong LCY, Badaloo GH, Saumtally S, Domaingue R (2000) The genetics of rust resistance in sugar cane seedling populations. Theoretical and Applied Genetics 100, 557–563.
Robert-Seilaniantz A, Navarro L, Bari R, Jones JDG (2007) Pathological hormone imbalances. Current Opinion in Plant Biology 10, 372–379.
| Pathological hormone imbalances.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXosFGrsbw%3D&md5=3fa65ba27b4530649a8f9ef6173d0b75CAS |
Rohlf FJ (2000) ‘NTSYS-PC Numerical Taxonomy and Multivariate Analysis System. Version 2.1.’ (Exeter Software: New York)
Ryan CC, Egan BT (1989) Rust. In ‘Diseases of sugarcane: major diseases’. (Eds C Ricaud, BT Egan, AGJ Gillaspie, CG Hughes) pp. 189–210. (Elsevier: Amsterdam, the Netherlands)
Siddiqui SH, Javed MA (1982) Mutation breeding in sugarcane (Saccharum sp. hybrid) by gamma irradiation of cuttings and tissue culture. In ‘Induced mutations in vegetatively propagated plants. II. Joint FAO/IAEA Div. of Isotope and Radiation Applications of Atomic Energy for Food and Agricultural Development’. Vienna, Austria. pp. 155–166. (IAEA)
Singh G, Sandhu SK, Meeta M, Singh K, Gill R, Gosal SS (2008) In vitro induction and characterization of somaclonal variation for red rot and other agronomic traits in sugarcane. Euphytica 160, 35–47.
| In vitro induction and characterization of somaclonal variation for red rot and other agronomic traits in sugarcane.Crossref | GoogleScholarGoogle Scholar |
Sreenivasan TV, Jalaja NC (1998) Induced mutations and somaclonal variation in sugarcane. In ‘Somaclonal variation and induced mutations in crop improvement’. (Eds S Mohan, DS Brar, S Ahloowalia) pp. 421–446. (Kluwer Academic Publisher: Dordrecht, the Netherlands)
Sun T (2008) Gibberellin metabolism, perception and signaling pathways in Arabidopsis. ‘The Arabidopsis Book 6’. e0103.2008 (The American Society of Plant Biologists: Rockville, MD) www.aspb.org/publications/arabidopsis/
Tai PYP, Miller JD, Dean JL (1981) Inheritance of resistance to rust in sugar cane. Field Crops Research 4, 261–268.
| Inheritance of resistance to rust in sugar cane.Crossref | GoogleScholarGoogle Scholar |
Van Dillewijn C (1952) ‘Botany of sugarcane.’ (The Chronica Botanica Co.: Waltham, MA)
Vos P, Hogers R, Bleeker M, Reijans M, Van de Lee T, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Research 23, 4407–4414.
| AFLP: a new technique for DNA fingerprinting.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2MXpslensbo%3D&md5=18c34dfd6f0203874baf7d5e81b0610bCAS |
Williams ND, Miller JD, Klindworth DL (1992) Induced mutations of a genetic suppressor of resistance to wheat stem rust. Crop Science 32, 612–616.
| Induced mutations of a genetic suppressor of resistance to wheat stem rust.Crossref | GoogleScholarGoogle Scholar |
Zambrano AY, Demey JR, Fuchs M, Gonzalez V, Rea R, De Sousa O, Gutierrez Z (2003) Selection of sugarcane mutants resistant to SCMV. Plant Science 165, 221–225.
| Selection of sugarcane mutants resistant to SCMV.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3sXks1Wlt70%3D&md5=2a2313aba20ff730c308ba51bfa81f15CAS |
