Diversity of seed protein among the Australian narrow-leafed lupin (Lupinus angustifolius L.) cultivars
Shahidul Islam A , Wujun Ma B C , Junhong Ma B , Bevan J. Buirchell B , Rudi Appels C and Guijun Yan A D
+ Author Affiliations
- Author Affiliations
A School of Plant Biology, Faculty of Natural and Agricultural Sciences and The UWA Institute of Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
B Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
C Centre for Comparative Genomics, Murdoch University, 90 South Street, Murdoch, WA 6150, Australia.
D Corresponding author. Email: guijun.yan@uwa.edu.au
Crop and Pasture Science 62(9) 765-775 https://doi.org/10.1071/CP11046
Submitted: 24 February 2011 Accepted: 24 September 2011 Published: 10 November 2011
Abstract
Narrow-leafed lupin (NLL) is one of the major legume crops in Australian farming systems which is largely used as animal feed. Several modern cultivars have been developed through breeding making NLL feasible for use as human food. Significant health benefits have been recognised for NLL. The current study characterised protein polymorphism among 25 Australian cultivars through mass spectrometry (MALDI-TOF) with the aim of developing molecular breeding strategies to improve protein quality and content. A total of 364 seed protein mass peaks were clearly identified by MALDI-TOF and 50 protein mass peaks were cultivar specific. In addition, 9 protein mass peaks were found present in all cultivars and 61 protein mass peaks present in 2–3 cultivars only. Phylogenic analysis based on the protein profile categorised the cultivars into 2 major groups, which are broadly supported by pedigree information. The low proportion (2.4%) of common protein mass peaks among the cultivars suggested a high level of diversity in seed protein of NLL.
Additional keywords: breeding, diversity, MALDI-TOF, phylogeny, protein profiling.
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