Genetic mapping and QTL analysis of disease resistance traits in the barley population Baudin × AC Metcalfe
Mehmet Cakir A H , Sanjiv Gupta A B , Chengdao Li B , Matthew Hayden C , Diane E. Mather D , Gary A. Ablett E , Greg J. Platz F , Sue Broughton B , Ken J. Chalmers D , Robert Loughman B , Michael G. K. Jones A and Reg C. M. Lance G
+ Author Affiliations
- Author Affiliations
A WA State Agricultural Biotechnology Centre, Faculty of Science, Engineering and Sustainability, Murdoch University, Murdoch, WA 6150, Australia.
B Department of Agriculture and Food Western Australia, South Perth, WA 6151, Australia.
C Department of Primary Industries, Victorian AgriBiosciences Center, Bundoora, Vic. 3083, Australia.
D School of Agriculture, Food and Wine, University of Adelaide, Glen Osmond, SA 5064, Australia.
E Centre for Plant Conservation Genetics, Southern Cross University, Lismore, NSW 2480, Australia.
F Queensland Department of Employment, Economic Development and Innovation, Hermitage Research Station, Warwick, Qld 4370, Australia.
G InterGrain Wheat Breeding Company, South Perth, WA 6151, Australia.
H Corresponding author. Email: m.cakir@murdoch.edu.au
Crop and Pasture Science 62(2) 152-161 https://doi.org/10.1071/CP10154
Submitted: 7 May 2010 Accepted: 23 December 2010 Published: 17 February 2011
Abstract
A genetic map of barley (Hordeum vulgare L.) with 163 amplified fragment length polymorphism and 69 simple sequence repeat (SSR) markers was constructed using a population of 178 doubled haploid lines from a cross between the varieties ‘Baudin’ and ‘AC Metcalfe’. Linkage groups were assigned to barley chromosomes using published map locations of SSR markers as reference points. The total length of the map was 1307.2 cM with an average interval length of 5.6 cM between markers. The genetic map was used to locate quantitative trait loci (QTLs) for several disease resistance traits. The population was tested for spot type net blotch, caused by Pyrenophora teres f. maculata, and net type net blotch, caused by Pyrenophora teres f. teres, in greenhouse experiments and in a range of field environments in Western Australia and Queensland. The response of the lines to leaf rust (caused by Puccinia hordei L.) at adult plant growth stages was evaluated in Western Australia. Significant marker–trait associations were found for seedling resistance and symptom severity in adult plants to these diseases. A new locus conferring resistance to P. teres f. maculata at both seedling and adult plant stages was detected on the short arm of chromosome 6H. From the seedling testing against P. teres f. teres, five highly repeatable QTLs were detected, on chromosomes 2HS, 2HL, 3HS, 4HL, and 6HS. For leaf rust, one highly significant QTL was detected on chromosome 2HL. The markers within these QTL regions present an opportunity for marker-assisted selection for these traits in barley-breeding programs.
Additional keywords: AFLP, barley, leaf rust, net type net blotch, spot type net blotch, SSR.
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