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Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Variation in grain β-glucan due to site, cultivar and nitrogen fertiliser in Western Australia

Blakely H. Paynter A C and Stefan E. Harasymow B
+ Author Affiliations
- Author Affiliations

A Department of Agriculture and Food Western Australia, Centre for Cropping Systems, PO Box 483, Northam, WA 6401, Australia.

B Department of Agriculture and Food Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.

C Corresponding author. Email: blakely.paynter@agric.wa.gov.au

Crop and Pasture Science 61(12) 1017-1026 https://doi.org/10.1071/CP10146
Submitted: 30 April 2010  Accepted: 4 November 2010   Published: 8 December 2010

Abstract

To adjust to projected changes in the future climate, maltsters are looking to reduce their water use. One option is to reduce the number of periods of water immersion during steeping from two to one. This might be possible if cultivars with very low grain β-glucan are used, as high β-glucan concentrations can restrict water penetration into the endosperm and the speed of germination. This study compared the grain β-glucan and various grain quality traits of nine two-row, Australian barley cultivars when grown at three sites with four rates of nitrogen (N) in Western Australia. Significant differences in grain β-glucan were found. Of the main factors, cultivar was found to have the largest effect on grain β-glucan followed by site, with N having the smallest effect. Grain β-glucan increased with increasing N application at two of the three sites. Not all cultivars responded similarly to N application. Grain with low β-glucan concentration generally had lower hectolitre weights, higher screenings, lower grain protein and was softer than grain with high grain β-glucan. There was no correlation between grain β-glucan and average grain weight, grain brightness or malt extract. The implication of these interactions and correlations in the breeding of very low grain β-glucan cultivars suited to single steep malting is discussed.

Additional keywords: (1→3),(1→4)-β-D-glucan, breeding, grain quality, malting.


References

Aastrup S (1979) The effect of rain on β-glucan content in barley grains. Carlsberg Research Communications 44, 381–393.
The effect of rain on β-glucan content in barley grains.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3cXkt1alsLg%3D&md5=63c0730b63d68f6a630a37e1ab936e12CAS |

Aastrup S (1983) Selection and characterization of low β-glucan mutants from barley. Carlsberg Research Communications 48, 307–316.
Selection and characterization of low β-glucan mutants from barley.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL2cXms1GgsQ%3D%3D&md5=da3c97d3ce469b4235b4cce50111697bCAS |

Åman P, Graham H (1987) Analysis of total and insoluble mixed-linked (1→3),(1→4)-β-D-glucans in barley and oats. Journal of Agricultural and Food Chemistry 35, 704–709.
Analysis of total and insoluble mixed-linked (1→3),(1→4)-β-D-glucans in barley and oats.Crossref | GoogleScholarGoogle Scholar |

Anker-Nilssen K, Sahlstrøm S, Knutsen SH, Holtekjølen AK, Uhlen AK (2008) Influence of growth temperature on content, viscosity and relative molecular weight of water-soluble β-glucans in barley (Hordeum vulgare L.). Journal of Cereal Science 48, 670–677.
Influence of growth temperature on content, viscosity and relative molecular weight of water-soluble β-glucans in barley (Hordeum vulgare L.).Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlagtbfO&md5=cc2696494f942e545be3cce4b1a10009CAS |

Bamforth CW (1982) Barley β-glucans – their role in malting and brewing. Brewers Digest 57, 22–28.

Bamforth CW (1985) Biochemical approaches to beer quality. Journal of the Institute of Brewing 91, 154–160.

Bamforth CW, Martin HL (1981) β-glucan and β-glucan solubilise in malting and brewing. Journal of the Institute of Brewing 87, 365–371.

Barber MG, Jackson EA, Smith DB (1994) Total and individual barley (1–3),(1–4)-D-β-glucanase activities in some green and kilned malts. Journal of the Institute of Brewing 100, 91–97.

Bendelow VM (1975) Determination of non-starch polysaccharides in barley breeding programmes. Journal of the Institute of Brewing 81, 127–130.

Bourne DT, Wheeler RE (1984) Environmental and varietal differences in total β-glucan contents of barley and the effectiveness of its breakdown under different malting conditions. Journal of the Institute of Brewing 90, 306–310.

Bureau of Meteorology (2010) Weather Station Data. Available at: www.bom.gov.au/climate/ (accessed March 2010).

Chandra GS, Proudlove MO, Baxter ED (1999) The structure of barley endosperm – an important determinant of malt modification. Journal of the Science of Food and Agriculture 79, 37–46.
The structure of barley endosperm – an important determinant of malt modification.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXhtlait7c%3D&md5=0e9ea6969262391678bb9fc1e8b156edCAS |

Coles GD, Jamieson PD, Haslemore RM (1991) Effect of moisture stress on malting quality in Triumph barley. Journal of Cereal Science 14, 161–177.
Effect of moisture stress on malting quality in Triumph barley.Crossref | GoogleScholarGoogle Scholar |

Ehrich E, Kneip EZ (1962) The nature of the malting process. In ‘Barley and malt’. (Ed. AH Cook) pp. 316–321. (Academic Press: London)

Ellis RP, Swanston JS, Rubio A, Pérez-Vendrell AM, Romagosa I, Molina-Cano JL (1997) The development of β-glucanase and degradation of β-glucan in barley grown in Scotland and Spain. Journal of Cereal Science 26, 75–82.
The development of β-glucanase and degradation of β-glucan in barley grown in Scotland and Spain.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK2sXkslCntrw%3D&md5=65106817fcf3988d35376fb45a9bfda1CAS |

Fastnaught CE, Berglund PT, Holm ET, Fox GJ (1996) Genetic and environmental variation in β-glucan content and quality parameters of barley for food. Crop Science 36, 941–946.
Genetic and environmental variation in β-glucan content and quality parameters of barley for food.Crossref | GoogleScholarGoogle Scholar |

Fincher GB (1975) Morphology and chemical composition of barley endosperm cell walls. Journal of the Institute of Brewing 81, 116–122.

Fincher GB (1993) Cell wall metabolism in barley. In ‘Barley: genetics, biochemistry, molecular biology and biotechnology’. (Ed. PR Shewry) pp. 413–437. (CAB International: Wallingford, UK)

Gamlath J, Aldred GP, Panozzo JF (2008) Barley (1→3;1→4)-b-glucan and arabinoxylan content are related to kernel hardness and water uptake. Journal of Cereal Science 47, 365–371.
Barley (1→3;1→4)-b-glucan and arabinoxylan content are related to kernel hardness and water uptake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXislaktrs%3D&md5=270b4b2e99722aefcb7d50b12cc36e13CAS |

Güler M (2003) Barley grain β-glucan content as affected by nitrogen and irrigation. Field Crops Research 84, 335–340.
Barley grain β-glucan content as affected by nitrogen and irrigation.Crossref | GoogleScholarGoogle Scholar |

Hang A, Obert D, Inez A, Gironella N, Burton CS (2007) Barley amylose and β-glucan: their relationships to protein, agronomic traits, and environmental factors. Crop Science 47, 1754–1760.
Barley amylose and β-glucan: their relationships to protein, agronomic traits, and environmental factors.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2sXpsF2qsrk%3D&md5=a228fbf8adc0f61da02905e1486ccbeeCAS |

Henry RJ (1985) A comparative study of the total-β-glucan contents of some Australian barleys. Australian Journal of Experimental Agriculture 25, 424–427.
A comparative study of the total-β-glucan contents of some Australian barleys.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28XjsVam&md5=6e0755c9b90798909c72d0f3bf62ab71CAS |

Henry RJ (1986) Genetic and environmental variation in the pentosan and β-glucan contents of barley, and their relation to malting quality. Journal of Cereal Science 4, 269–277.
Genetic and environmental variation in the pentosan and β-glucan contents of barley, and their relation to malting quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL28XkvFCmsbY%3D&md5=d9ef65fe03b76ac7b5670c5b185986f4CAS |

Henry RJ (1988) Changes in β-glucan and other carbohydrate components of barley during malting. Journal of the Science of Food and Agriculture 42, 333–341.
Changes in β-glucan and other carbohydrate components of barley during malting.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXhsVyrtrs%3D&md5=3b260bd346e31c6796aa0415851f4bc5CAS |

Henry RJ (1989) Factors influencing the rate of modification of barleys during malting. Journal of Cereal Science 10, 51–59.
Factors influencing the rate of modification of barleys during malting.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1MXlsFajt78%3D&md5=a801faae352e0e06cfe8d9bd02055f5dCAS |

Henry RJ, Cowe IA (1990) Factors influencing the hardness (milling energy) and malting quality of barley. Journal of the Institute of Brewing 96, 135–136.

Holtekjølen AK, Uhlen AK, Bråthen E, Sahlstrøm S, Knutsen SH (2006) Contents of starch and non-starch polysaccharides in barley varieties of different origin. Food Chemistry 94, 348–358.
Contents of starch and non-starch polysaccharides in barley varieties of different origin.Crossref | GoogleScholarGoogle Scholar |

Iwami A, Kajiwara Y, Omori T (2003) Estimating barley character for shochu using a Single Kernel Characterization System (SKCS). Journal of the Institute of Brewing 109, 129–134.

Jackson GD, Berg RK, Kushnak GD, Blake TK, Yarrow GI (1994) Nitrogen effects on yield, beta-glucan content and other quality factors for oat and waxy hulless barley. Communications in Soil Science and Plant Analysis 25, 3047–3055.
Nitrogen effects on yield, beta-glucan content and other quality factors for oat and waxy hulless barley.Crossref | GoogleScholarGoogle Scholar |

Lehtonen M, Aikasalo R (1987) β-glucan in two- and six-rowed barley. Cereal Chemistry 64, 191–192.

Li J, Baga M, Rossnagel BG, Legge WG, Chibbar RN (2008) Identification of quantitative trait loci for β-glucan concentration in barley grain. Journal of Cereal Science 48, 647–655.
Identification of quantitative trait loci for β-glucan concentration in barley grain.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXhtlagtbbK&md5=18eb969400d6d3115d249b445b8209e5CAS |

MacGregor AW (1991) The effect of barley structure and composition on malt quality. In ‘Proceedings of the 23rd Congress of European Brewery Convention’. Lisbon, Portugal. pp. 37–50. (IRL Press: Oxford, UK)

MacGregor AW, Fincher GB (1993) Carbohydrates of the barley grain. In ‘Barley: chemistry and technology’. (Eds AW MacGregor, RS Bhatty) pp. 73–130. (American Association of Cereal Chemists: St Paul, MN)

Molina-Cano JL, Francesch M, Pérez-Vendrell AM, Ramo T, Voltas J, Brufau J (1997) Genetic and environmental variation in malting and feed barley quality. Journal of Cereal Science 25, 37–47.
Genetic and environmental variation in malting and feed barley quality.Crossref | GoogleScholarGoogle Scholar |

Molina-Cano JL, Ramo T, Ellis RP, Swanston JS, Bain H, Uribe-Echeverria T, Pérez-Vendrell AM (1995) Effect of grain composition on water uptake by malting barley: a genetic and environmental study. Journal of the Institute of Brewing 101, 79–83.

Molina-Cano JL, Roca de Togores F, Royo C, Pérez A (1989) Fast germinating low β-glucan mutants induced in barley with improved malting quality. Theoretical and Applied Genetics 78, 748–754.
Fast germinating low β-glucan mutants induced in barley with improved malting quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK3cXpsFOgsw%3D%3D&md5=f7924d4200a718d7ffda17c76fadb616CAS |

Molina-Cano JL, Sopena A, Polo JP, Bergareche C, Moralejo MA, Swanston JS, Glidewell SM (2002) Relationships between barley hordeins and malting quality in a mutant of cv. Triumph. II. Genetic and environmental effects on water uptake. Journal of Cereal Science 36, 39–50.
Relationships between barley hordeins and malting quality in a mutant of cv. Triumph. II. Genetic and environmental effects on water uptake.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD38Xnt1agu7c%3D&md5=337da0163177e2d0b52ef04b60597791CAS |

Molina-Cano JL, Sopena A, Swanston JS, Casas AM, Moralejo MA, Ubieto A, Lara I, Pérez-Vendrell AM, Romagosa I (1999) A mutant induced in the malting barley cv. Triumph with reduced dormancy and ABA response. Theoretical and Applied Genetics 98, 347–355.
A mutant induced in the malting barley cv. Triumph with reduced dormancy and ABA response.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1MXivVCls7k%3D&md5=465550df36f9ce5a2b7baae0db3ac880CAS |

Morgan AG, Riggs TJ (1981) Effects of drought on yield and grain and malt characteristics in spring barley. Journal of the Science of Food and Agriculture 32, 339–346.
Effects of drought on yield and grain and malt characteristics in spring barley.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL3MXkvFeru7g%3D&md5=9bcb01f42cd1f9e50925a57c70858bd6CAS |

Narasimhalu P, Kong D, Choo TM, Ferguson T, Therrien MC, Ho KM, May KW, Jui P (1995) Effects of environmental and cultivar on total mixed-linkage β-glucan content in eastern and western Canadian barleys (Hordeum vulgare L.). Canadian Journal of Plant Science 75, 371–376.

Oscarsson M, Andersson R, Åman P, Olofsson S, Jonsson A (1998) Effects of cultivar, nitrogen fertilization rate and environment on yield and grain quality of barley. Journal of the Science of Food and Agriculture 78, 359–366.
Effects of cultivar, nitrogen fertilization rate and environment on yield and grain quality of barley.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaK1cXnvVaru70%3D&md5=53396ea663c842c56bfb58fe93b1e53cCAS |

Özkara R, Basman A, Koksel H, Celik S (1998) Effects of cultivars and environment on β-glucan content and malting quality of Turkish barleys. Journal of the Institute of Brewing 104, 217–220.

Payne RW, Harding SA, Murray DA, Soutar DM, Baird DB, Glaser AI, Channing IC, Welham SJ, Gilmour AR, Thompson R, Webster R (2008) ‘Genstat Reference Manual Release 11.’ (VSN International: Hemel Hempstead, UK)

Paynter BH, Portmann P (2005) Hardness and pearling characteristics of Hamelin and Baudin barley. In ‘Proceedings of the 12th Australian Barley Technical Symposium’. 10–13 Sept. 2005, Hobart, Tas. Available at: www.cdesign.com.au/proceedings_abts2005/posters%20(pdf)/poster_blakely4.pdf (accessed October 2010)

Pérez-Vendrell AM, Brufau J, Molina-Cano JL, Francesch M, Guasch J (1996) Effects of cultivar and environment on β-(1,3)-(1,4)-D-glucan content and acid extract viscosity of Spanish barleys. Journal of Cereal Science 23, 285–292.
Effects of cultivar and environment on β-(1,3)-(1,4)-D-glucan content and acid extract viscosity of Spanish barleys.Crossref | GoogleScholarGoogle Scholar |

Psota V, Vejražka K, Famĕra O, Hrčka M (2007) Relationship between grain hardness and malting quality of barley (Hordeum vulgare L.). Journal of the Institute of Brewing 109, 236–244.

Schoknecht N (2002) Soil groups of Western Australia. A simple guide to the main soils of Western Australia. Resource Management Technical Report 246. Department of Agriculture, Western Australia, South Perth.

Stewart D (2007) Barley breeders and maltsters – working together to shift the goal posts – an industry discussion paper. In ‘Proceedings of the 13th Australian Barley Technical Symposium’. 26–30 August 2007, Fremantle, W. Aust. pp. 79–82. (Promaco Conventions Pty Ltd: Canning Bridge, WA)

Stuart IM, Loi L, Fincher GB (1988) Varietal and environmental variation in (1→3,1→4)-β-glucan levels and (1→3,1→4)-β-glucanase potential in barley: relationships to malting quality. Journal of Cereal Science 7, 61–71.
Varietal and environmental variation in (1→3,1→4)-β-glucan levels and (1→3,1→4)-β-glucanase potential in barley: relationships to malting quality.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DyaL1cXhs1KjtrY%3D&md5=ed8d179cc7d5792e90f50a9c8efba798CAS |

Tiwari U, Cummins E (2008) A predictive model of the effects of genotypic, pre- and postharvest stages on barley levels. Journal of the Science of Food and Agriculture 88, 2277–2287.
A predictive model of the effects of genotypic, pre- and postharvest stages on barley levels.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD1cXht1Ciu7rO&md5=d0562838483d6f66fc473468b1fe9ec7CAS |

Tohno-oka T, Kawada N, Yoshioka T (2004) Relationship between grain hardness and endosperm cell wall polysaccharides in barley. In ‘Proceedings of the 9th International Barley Genetics Symposium’. 20–26 June 2004, Brno, Czech Republic. (Eds IJ Spunar, J Janikova) pp. 595–600. (Agricultural Research Institute Kromeriz Ltd: Brno, Czech Republic)

Wallwork MAB, Logue SJ, MacLeod LC, Jenner CE (1998) Effects of a period of high temperature during grain filling on the grain growth characteristics and malting quality of three Australian malting barleys. Australian Journal of Agricultural Research 49, 1287–1296.
Effects of a period of high temperature during grain filling on the grain growth characteristics and malting quality of three Australian malting barleys.Crossref | GoogleScholarGoogle Scholar |

Wang J, Zhang G, Chen J, Wu F (2004) The changes of β-glucan content and β-glucanase activity in barley before and after malting and their relationships to malt qualities. Food Chemistry 86, 223–228.
The changes of β-glucan content and β-glucanase activity in barley before and after malting and their relationships to malt qualities.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD2cXhs1Cru7o%3D&md5=4c91509c0974e4f62261be54f8b66493CAS |

Zhang G, Chen J, Wang J, Ding S (2001) Cultivar and environmental effects on (1→3,1→4)-β-D-glucan and protein content in malting barley. Journal of Cereal Science 34, 295–301.
Cultivar and environmental effects on (1→3,1→4)-β-D-glucan and protein content in malting barley.Crossref | GoogleScholarGoogle Scholar | 1:CAS:528:DC%2BD3MXpt12rsbY%3D&md5=0c30c42f9f513eb17bc656722feb0029CAS |