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RESEARCH ARTICLE
Contents Vol 61(6)

Allelic diversity in a novel gene pool of canola-quality Brassica napus enriched with alleles from B. rapa and B. carinata

S. Chen A D E , J. Zou B D , W. A. Cowling A C and J. Meng B E
+ Author Affiliations
- Author Affiliations

A School of Plant Biology and International Centre for Plant Breeding Education and Research, The University of Western Australia, Crawley, WA 6009, Australia.

B National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China.

C Canola Breeders Western Australia Pty Ltd, Locked Bag 888, Como, WA 6952, Australia.

D These authors contributed equally to this paper.

E Corresponding authors. Email: chens@cyllene.uwa.edu.au; jmeng@mail.hzau.edu.cn

Crop and Pasture Science 61(6) 483-492 https://doi.org/10.1071/CP09327
Submitted: 18 November 2009  Accepted: 1 February 2010   Published: 1 June 2010

Abstract

Brassica napus is an amphidiploid with genome AACC and is relatively deficient in genetic diversity. The abundant genetic diversity in other A- and C-genome Brassica species is a valuable resource to expand the narrow gene pool of B. napus. Recently the Ar genomic components from Chinese B. rapa (ArAr) and Cc genomic components from Ethiopian mustard B. carinata (BBCcCc) were introgressed into B. napus through interspecific hybridisation, and the Ar/Cc components were enriched through two generations of molecular marker-assisted selection. In this study, the simple sequence repeat (SSR) allelic diversity of 29 of these new-type B. napus lines, 12 from the first generation and 17 from the second generation, was compared with 66 international B. napus varieties from Australia, China and other countries. Hierarchical clustering and two-dimensional multidimensional scaling revealed that second generation lines and a few first generation lines, all selected for high Ar/Cc components, formed a unique population that was distantly separated from international B. napus. This novel gene pool had significantly higher richness of private SSR alleles and more alleles per SSR marker than the international B. napus varieties. The new-type B. napus lines showed variation in agronomic traits beyond the canola-quality B. napus parent. Many of the lines had low erucic acid and low glucosinolates in the seed (canola quality), indicating that they could be utilised immediately in canola breeding programs.

Additional keywords: allelic distinctiveness, allelic diversity, canola, gene pool, interspecific introgression, molecular breeding.


Acknowledgments

This work was supported financially by the National Natural Science Foundation of China (project code: 30830073) and the UWA Research Development Award.


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