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RESEARCH ARTICLE
Contents Vol 61(2)

Genetics of late maturity α-amylase in a doubled haploid wheat population

M. K. Tan A D E , A. P. Verbyla B E , B. R. Cullis C E , P. Martin C , A. W. Milgate C and J. R. Oliver C
+ Author Affiliations
- Author Affiliations

A Industry and Investment NSW, Elizabeth Macarthur Agricultural Institute, Private Bag 4008, Narellan, NSW 2567, Australia.

B School of Agriculture, Food and Wine, The University of Adelaide and Statistical Bioinformatics, Mathematical and Information Sciences, CSIRO, Private Mail Bag 1, Glen Osmond, SA 5064, Australia.

C Industry and Investment NSW, Wagga Wagga Agricultural Institute, Private Mail Bag, Wagga Wagga, NSW 2650, Australia.

D Corresponding author. Email: mui-keng.tan@industry.nsw.gov.au

E MKT, APV and BRC contributed almost equally to this work.

Crop and Pasture Science 61(2) 153-161 https://doi.org/10.1071/CP09239
Submitted: 14 August 2009  Accepted: 8 December 2009   Published: 8 February 2010

Abstract

Late maturity α-amylase (LMA) in wheat is a defect where high-isoelectric point (pI) α-amylase accumulates in the ripening grain. Wheat genotypes vary in expression from zero to high levels of α-amylase, the latter with detrimental consequences on their use for value-added end products. Expression in each genotype is characterised by varying numbers of grains affected and different levels in each grain. Analysis of a doubled haploid (DH) population (188 lines) from WW1842 × Whistler has identified significant QTL on chromosomes 2DL, 3A, 3B, 3D, 4B, 4D, 5DS and 5BL. The 4B LMA allele (P < 0.0001) from Whistler is closely linked to the QTL for the ‘tall’ allele (P < 0.0001) of the Rht-B1 gene. The 4D LMA QTL (P < 0.0001) in WW1842 co-locates with the QTL for the ‘tall’ allele (P < 0.0001) of the Rht-D1 gene. This study has shown for the first time that a DH cross between two semi-dwarf cultivars with low or no LMA produces ~25% of progeny lines of the ‘tall’ genotypes with a high frequency of LMA. This is attributed to the large additive positive effects from the combination of one recessive ‘tall’ Rht-B1 gene and one recessive ‘tall’ Rht-D1 gene. High-yielding semi-dwarf genotypes with different combinations of Rht-B1 and Rht-D1 alleles which have very low or non-existent LMA expression (e.g. WW1842 and Whistler) may meet industry criteria for registration as commercial wheat varieties. However, when they are used as breeding lines, the cross produces some progeny genotypes with severe levels of LMA. These LMA genotypes comprise the gibberellic acid-sensitive ‘tall’ progenies and a very small proportion of semi-dwarfs. Thus, it is of paramount importance to screen the defect in wheat breeding programs. The suite of QTL identified for LMA will enable the use of marker assisted selection in the pyramiding of the beneficial QTL to maximise yield and minimise (or eliminate) LMA in semi-dwarf genotypes.

Additional keywords: genetic mapping, gibberellic acid, Rht genes, yield, marker assisted selection, plant height, QTL, semi-dwarfs.


Acknowledgments

The authors gratefully acknowledge Renu Srivastava, Rui Jun Li, Jiai Chen, and Nilufa Sultana for their excellent technical support in the project, and Dr Neil Howes and Dr Zhao Xiaochun for invaluable assistance in glasshouse trials. We thank Value-Added Wheat CRC, Australia (now terminated), and GRDC for financial support.


References


Akbari M, Wenzl P, Caig V, Carling J, Xia L, Yang S, Uszynski G, Mohler V, Lehmensiek A, Kuchel H, Hayden MJ, Howes N, Sharp P, Vaughan P, Rathmell B, Huttner E, Kilian A (2006) Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theoretical and Applied Genetics 113, 1409–1420.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Börner A, Plaschke J, Korzun V, Worland AJ (1996) The relationships between the dwarfing genes of wheat and rye. Euphytica 89, 69–75.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Börner A, Röder M, Korzun V (1997) Comparative molecular mapping of GA insensitive Rht loci on chromosomes 4B and 4D of common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 95, 1133–1137.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Borojevic K, Borojevic K (2005) The transfer and history of “reduced height genes” (Rht) in wheat from Japan to Europe.  Journal of Heredity 96, 455–459.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Broman KW , Wu H , Churchill G , Yandell B (2006) qtl: Tools for analyzing QTL experiments . R package version 1.05-2.

Butler D, Tan MK, Cullis BR (2009) Improving the accuracy of selection for late maturity α-amylase in wheat using multi-phase designs. Crop & Pasture Science 60, 1202–1208.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Caldwell KS, Dvorak J, Lagudah ES, Akhunov E, Luo MC, Wolters P, Powell W (2004) Sequence polymorphism in polyploidy wheat and their D-genome diploid ancestor. Genetics 167, 941–947.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Ellis MH, Spielmeyer W, Gale KR, Rebetzke GJ, Richards RA (2002) “Perfect” markers for the Rht-B1b and Rht-D1b dwarfing genes in wheat. Theoretical and Applied Genetics 105, 1038–1042.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Farrell AD, Kettlewell PS (2008) The effect of temperature shock and grain morphology on alpha-amylase in developing wheat grain. Annals of Botany 102, 287–293.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Flintham JE, Angus WJ, Gale MD (1997a) Heterosis, overdominance for grain yield, and alpha-amylase activity in F-1 hybrids between near-isogenic Rht dwarf and tall wheats. Journal of Agricultural Science 129, 371–378.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Flintham JE, Börner A, Worland AJ, Gale MD (1997b) Optimizing wheat grain yield: Effects of Rht (gibberellin-insensitive) dwarfing genes. Journal of Agricultural Science 128, 11–25.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gale MD, Gregory RS (1977) A rapid method for early generation selection of dwarf genotypes in wheat. Euphytica 26, 733–738.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gale MD, Marshall GA (1975) The nature and genetic control of gibberellin insensitivity in dwarf wheat grain. Heredity 35, 55–65.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gale MD, Marshall GA, Rao MV (1981) A classification of the Norin 10 and Tom Thumb dwarfing genes in British, Mexican, Indian and other hexaploid bread wheat varieties. Euphytica 30, 355–361.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gale MD, Law CN, Chojecki AJ, Kempton RA (1983) Genetic control of α-amylase production in wheat. Theoretical and Applied Genetics 64, 309–316.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Gold CM , Duffus CM (1993) The effect of gibberellic acid-insensitive dwarfing genes on pre-maturity α-amylase and sucrose relationship during grain development in wheat. In ‘Preharvest sprouting in cereals 1992’. (Eds MK Walker-Simmons, JL Reid) pp. 171–177. (AACC: St Paul, MN)

Gold CM , Duffus CM (1996) Pre-Maturity alpha-amylase production in cereal grains. In ‘Preharvest sprouting in cereals 1995’. (Eds K Noda, DJ Mares) pp. 363–369. (Center for Academic Societies: Osaka, Japan)

Gooden J , Batey I , Miskelly D , Howes N , Wrigley C (2007) Screening for field expression of late-maturity α-amylase (LMA). Value Added Wheat CRC Report No. 70. http://ses.library.usyd.edu.au/handle/2123/2754

Hayden MJ, Stephenson P, Logojan AM, Khatkar D, Rogers C, Elsden J, Koebner RMD, Snape JW, Sharp PJ (2006) Development and genetic mapping of sequence-tagged microsatellites (STMs) in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 113, 1271–1281.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Law CN, Snape JW, Worland AJ (1978) The genetical relationship between height and yield in wheat. Heredity 40, 133–151.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lunn GD, Major BJ, Kettlewell PS, Scott RK (2001) Mechanisms leading to excess alpha-amylase activity in wheat (Triticum aestivum L.) grain in the UK. Journal of Cereal Science 33, 313–329.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Manly KF, Cudmore RH, Meer JM (2001) Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12, 930–932.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Mares DJ, Mrva K (2008) Late-maturity alpha-amylase: low falling number in wheat in the absence of preharvest sprouting. Journal of Cereal Science 47, 6–17.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

McNeil MD, Diepeveen D, Wilson R, Barclay I, McLean R, Chalhoub B, Appels R (2009) Haplotype analyses in wheat for complex traits: tracking the chromosome 3B and 7B regions associated with late maturity alpha amylase (LMA) in breeding programs. Crop & Pasture Science 60, 463–471.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Mrva K, Mares DJ (1996) Expression of late maturity alpha amylase in wheat containing gibberellic acid insensitivity genes. Euphytica 88, 69–76.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Mrva K, Mares DJ (2001) Quantitative trait locus analysis of late maturity α-amylase in wheat using the doubled haploid population Cranbrook × Halberd. Australian Journal of Agricultural Research 52, 1267–1273.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Mrva K , Mares DJ , Williams KJ , Cheong J (2004) Molecular markers associated with late maturity α-amylase (LMA) in wheat. In ‘Proceedings of the 53rd Australian Cereal Chemistry Conference’. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 150–151. (RACI Cereal Chemistry Division: North Melbourne, Vic.)

Norman HA, Black M, Chapman JM (1982) The induction of sensitivity to gibberellin in aleurone tissue of developing wheat grains. II. Evidence for temperature-dependent membrane transitions. Planta 154, 578–586.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

R-Development Core Team (2006) ‘R: a language and environment for statistical computing.’ (R Foundation for Statistical Computing: Vienna, Austria)

Röder MS, Korzun V, Wendehake K, Plaschke J, Tixier M, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |
open url image1

Smith AB, Lim P, Cullis BR (2006) The design and analysis of multi-phase plant breeding experiments.  Journal of Agricultural Science 144, 393–409.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Snape JW, Law CN, Worland AJ (1977) Whole chromosome analysis of height in wheat. Heredity 38, 25–36.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109, 1105–1114.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microstellite (SSR) markers in wheat. Theoretical and Applied Genetics 110, 550–560.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | open url image1

Tan MK, Medd RW (2002) Characterisation of the acetolactate synthase (ALS) gene of Raphanus raphanistrum L. and the molecular assay of mutations associated with herbicide resistance. Plant Science 163, 195–205.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1

Taylor JD , Diffey S , Verbyla AP , Cullis BR (2009) Wgaim: whole Genome Average Interval Mapping for QTL detection using mixed models . R package version 0.2–1.

Verbyla AP, Cullis BR, Thompson R (2007) The analysis of QTL by simultaneous use of the full linkage map. Theoretical and Applied Genetics 116, 95–111.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Verity JCK, Hac L, Skerritt JH (1999) Development of a field enzyme-linked immunosorbent assay (ELISA) for detection of alpha-amylase in preharvest-sprouted wheat. Cereal Chemistry 76, 673–681.
| Crossref | GoogleScholarGoogle Scholar | CAS | open url image1