Field studies on the biofumigation of take-all by Brassica break crops

Abstract

Biofumigation refers to the suppression of soil-borne pathogens and pests by biocidal compounds released by Brassica crops when glucosinolates (GSL) in their residues decay in soil. We conducted field studies at 2 sites to investigate the hypothesis that biofumigation by Brassica break crops would reduce inoculum of the take-all fungus Gaeumannomyces graminis var. tritici (Ggt) to lower levels than non-Brassica break crops, and thereby reduce Ggt infection and associated yield loss in subsequent wheat crops. High and uniform levels of Ggt were established at the sites in the first year of the experiments by sowing wheat with sterilised ryegrass seed infested with Ggt. Ggt inoculum declined more rapidly under Brassica crops than under linola and this reduction coincided with the period of root decay and reduced root glucosinolate concentrations around crop maturity. There was no consistent difference in inoculum reduction between canola (Brassica napus) and Indian mustard (Brassica juncea), nor between cultivars with high and low root GSL within each species. Despite significant inoculum reduction attributable to biofumigation, there were no differences in the expression of disease and associated impacts on the yield of subsequent wheat crops across the sites. Seasonal conditions, in particular the distribution of rainfall in both the summer–autumn fallow following the break crops and during the subsequent wheat crop, influenced inoculum survival and subsequent disease development. In wet summers, inoculum declined to low levels following all break crops and no extra benefit from biofumigation was evident. In dry summers the lower inoculum levels following brassicas persisted until the following wheat crops were sown but subsequent development of the disease was influenced more by seasonal conditions than by initial inoculum levels. Significant extra benefits of biofumigation were observed in one experiment where wheat was sown within the break crops to simulate grass weed hosts of Ggt. Under these circumstances there was greater reduction in Ggt inoculum under canola than linseed and an associated decrease in disease development. For host-dependent pathogens such as Ggt, we hypothesise that the benefits of biofumigation to subsequent wheat crops will therefore be restricted to specific circumstances in which inoculum is preserved during and after the break crops (i.e. dry conditions, grass hosts present) and where conditions in the following wheat crop lead to significant disease development (early sowing, wet autumn and spring, dry periods during grain filling).