Two genetic linkage maps of mungbean using RFLP and RAPD markers

Abstract

Two genetic linkage maps of mungbean derived from the cross Berken ACC 41 are reported. The F₂ map constructed from 67 individuals consisted of 110 markers (52 RFLP and 56 RAPD) that grouped into 12 linkage groups. The linked markers spanned a total map distance of 758.3 cM. A recombinant inbred (RI) population derived from the 67 F₂ individuals was used for the generation of an additional linkage map. The RI map, composed entirely of RAPD markers, consisted of 115 markers in 12 linkage groups. The linked markers spanned a total map distance of 691.7 cM. Using a framework set of RFLP markers, the F₂ map was compared with another F₂ mungbean map constructed in Minnesota. In general, the order of these markers was consistent between maps. Segregation distortion was observed for some markers. 14.5% (16/110) of mapped F₂ markers and 24% (28/115) of mapped RI markers segregated with distorted ratios. Segregation distortion occurred in each successive generation after the F₂ . The regions of distortion identified in the Australian maps did not coincide with regions of the Minnesota map.