Effect of pod burial, light, and temperature on seed softening in yellow serradella

Abstract

Studies were made on the preconditioning stage (which produces latent soft seeds) and the final stage of seed softening in newly ripened seeds of the GEH72-1A accession of yellow serradella (Ornithopus compressus L.). Pods grown at Yelbeni, Western Australia, in 1996 were collected in December and placed on the soil surface or buried at a depth of 0.5 cm at a site near Perth. Other pods were subjected to a gradual diurnal temperature fluctuation of 60/15°C in darkness in a laboratory chamber. Pod samples were taken from the field at intervals from January to June in 1997, and over 336 days from the 60/15°C treatment. Pods were broken into segments and the number of soft seeds determined. Numbers of latent soft seeds were then determined by subjecting residual hard seeds to 7 gradual diurnal temperature cycles of 48/15°C in darkness and retesting for permeability. In a second experiment, seeds preconditioned at the soil surface until 3 March were subjected to a range of light levels in the field in March before testing for permeability. The time taken for seeds to precondition under a range of constant temperatures between 30° and 70°C was determined in a third experiment.

Preconditioning commenced early in summer in both surface and buried seeds. All buried seeds that preconditioned completed the softening process to produce about 80% soft seeds, with most seeds softening in March when diurnal temperatures fluctuated between maxima of 45–50°C and minima of 10–20°C. Only 15% of the seeds at the soil surface softened so that relatively few preconditioned seeds completed the softening process. Preconditioning occurred more rapidly than did the completion of softening in the 60/15°C treatment, indicating that this temperature regime was above optimum for the final stage of softening. Reversal of the preconditioning process took place in the field as temperatures declined during May. Effects of reduced temperatures in causing this reversion were confirmed in the laboratory on seeds preconditioned at 60/15°C. The final stage of softening was inhibited in some seeds by light levels as low as 0.3% of daylight, and in all seeds at a light level between 5 and 25%. A close negative linear relation was obtained between the log of the time taken for 50% of seeds to precondition and the constant temperature treatment between 30°C and 70°C, with the rate of preconditioning doubling with every 5.2°C rise in temperature within this range.

Although many seeds preconditioned at the soil surface, the main constraint to completion of the seed softening process during autumn was the inhibitory effect of light.