Effect of recent red leaf virus infection on the distribution of ⁶⁵Zn, ⁴⁵Ca, and ⁵⁴Mn in subterranean clover

Abstract

Subterranean clover (Trifolium subterraneurn L. cv. Yarloop) was grown in nutrient solutions which were supplied with ⁴⁵Ca, ⁶⁵Zn, or ⁵⁴Mn either before (series A) or at the same time as (series B) the plants \+ere inoculated with subterranean clover red leaf virus (SCRLV) by means of the aphid vector Acyrthosiphon solani Kltb.

SCRLV infection affected the distribution of each isotope, but less in series A than in series B plants. In both series, movement of the isotopes from the petiole (P) into the lamina (L) was retarded. In series B this resulted in a significant decrease in the concentration of ⁴⁵Ca, ⁶⁵Zn, or ⁵⁴Mn in the lamina. The L/P ratios of concentration and the total contents of ⁶⁵Zn and ⁵⁴Mn in the leaves of SCRLV-infected plants from both series were also reduced. In addition SCRLV infection reduced the 54Mn concentration in the distal third more than in the remainder of the lamina.

SCRLV infection increased the dry weight of the lamina and/or the LIP ratio of dry weight of the younger leaves of the series B plants.