Male fertility restoration of wheat in Hordeum chilense cytoplasm is associated with 6HchS chromosome addition
A. C. Martín A B , S. G. Atienza A , M. C. Ramírez A , F. Barro A and A. Martín AA Departamento de Mejora Genética Vegetal, Instituto de Agricultura Sostenible (C.S.I.C.),Apdo. 4084, E-14080 Córdoba, Spain.
B Corresponding author. Email: a62maraa@uco.es
Australian Journal of Agricultural Research 59(3) 206-213 https://doi.org/10.1071/AR07239
Submitted: 18 June 2007 Accepted: 7 December 2007 Published: 11 March 2008
Abstract
We report a new cytoplasmic male sterility (CMS) source in bread wheat (Triticum aestivum L.) designated as msH1. CMS has been identified during the process of obtaining alloplasmic bread wheat in different Hordeum chilense Roem. Schultz. cytoplasms. It was observed that when using the H. chilense H1 accession, the corresponding alloplasmic line was male sterile. This alloplasmic wheat is stable under different environmental conditions and it does not exhibit developmental or floral abnormalities, showing only slightly reduced height and some delay in heading. On examining microsporogenesis in the alloplasmic line, it was found that different stages of meiosis were completed normally, but abnormal development occurred at the uninucleate-pollen stage at the first mitosis, resulting in failure of anther exertion and pollen abortion. Fertility restoration of the CMS phenotype caused by the H. chilense cytoplasm was associated with the addition of chromosome 6HchS from H. chilense accession H1. Thus, some fertility restoration genes appear to be located in this chromosome arm. Considering the features displayed by the msH1 system, we consider that it has a great potential for the development of viable technology for hybrid wheat production.
Additional keywords: cytoplasmic male sterility, fertility restoration, Hordeum chilense, wheat.
Acknowledgments
We thank Prof. T. R. Endo for information on the localisation of the EST marker used in this work on barley chromosome 6HS. A. C. Martín acknowledges the CSIC for a pre-doctoral fellowship. S. G. Atienza acknowledges financial support from the ‘Ramón y Cajal’ program of the Spanish Ministry of Education (MEC). This research was financed by project AGL2004-03361-CO2-02 and AGL2005-01381.
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