Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > AR07227
RESEARCH ARTICLE
Contents Vol 59(5)

Mining synthetic hexaploids for multiple disease resistance to improve bread wheat

F. C. Ogbonnaya A E F G , M. Imtiaz A , H. S. Bariana B , M. McLean A , M. M. Shankar C , G. J. Hollaway A , R. M. Trethowan B , E. S. Lagudah D and M. van Ginkel A E
+ Author Affiliations
- Author Affiliations

A Primary Industries Research Victoria (PIRVic), Department of Primary Industries, Private Bag 260, Horsham, Vic. 3401, Australia.

B University of Sydney Plant Breeding Institute-Cobbitty, PMB11, Camden, NSW 2570, Australia.

C Department of Agriculture, South Perth, WA 6151, Australia.

D CSIRO Division of Plant Industry, Canberra, ACT 2601, Australia.

E Molecular Plant Breeding Cooperative Research Centre, Bundoora, Vic. 3083, Australia.

F Current address: International Centre for Agricultural Research in the Dry Areas (ICARDA), PO Box 5466, Aleppo, Syria.

G Corresponding author. Email: F.Ogbonnaya@cgiar.org

Australian Journal of Agricultural Research 59(5) 421-431 https://doi.org/10.1071/AR07227
Submitted: 14 June 2007  Accepted: 14 November 2007   Published: 12 May 2008

Abstract

A collection of 253 synthetic hexaploid wheats (SHWs) produced from 192 Aegilops tauschii accessions and 39 elite durum varieties were studied to identify, characterise, and evaluate potentially untapped diversity of disease resistance in wheat. The diseases for which resistance was sought included cereal cyst nematode (CCN), root lesion nematode (RLN), Stagonospora nodorum blotch (SNB), Septoria tritici blotch (STB), and the 3 rusts, leaf rust, stem rust, and stripe rust, all important diseases of bread wheat worldwide, which can severely reduce wheat yield and quality. The SHWs exhibited a wide spectrum of resistance to the 8 pathogens. The frequency of disease-resistant SHWs ranged from 1% for one species of RLN (Pratylenchus neglectus), 3% and 10% for Septoria nodorum leaf and glume blotch, 10% for seedling resistance to yellow leaf spot, 16% for CCN, 21% for the second species of RLN (Pratylenchus thornei), 73% for Septoria tritici blotch, and 15%, 40%, and 24% for leaf rust, stem rust, and stripe rust, respectively. Five SHWs, Aus26860, Aus30258, Aus30294, Aus30301, and Aus30304, exhibited high levels of resistance to CCN, YLP, STB, LR, and SR, while 56 SHWs showed resistance to either 3 or 4 diseases. The genetics of resistance to CCN in some of the SHWs revealed that some of the accessions carry the same CCN gene(s) against pathotype Ha13, while others may carry different resistance gene(s). Additional studies were carried out to understand the relationship between the resistances identified in SHWs and the ones already present in common wheat, in particular the resistance genes Cre1 and Cre3 against CCN. The use of perfect markers associated with Cre1 and Cre3 suggested that some SHWs may carry a new CCN resistance gene(s), which could be deployed in breeding programs to increase the diversity of available resistance. The identification of SHWs with resistance to a range of diseases provides an opportunity to generate genetic knowledge and resistant germplasm to be used in future variety development.

Additional keywords: genetic diversity, synthetic hexaploid wheat, Aegilops tauschii, durum, Triticum aestivum.


Acknowledgments

The authors acknowledge financial support from the Grains Research and Development Corporation, the Department of Primary Industries Victoria, the Molecular Plant Breeding CRC, and the International Maize and Wheat Improvement Centre. They thank J. Wilson, M. S. McLean, S. P. Taylor, J. P. Thompson, and A. Milgate for their assistance with the biological assays.


References


Arraiano LS, Brown JKM (2006) Identification of isolate-specific and partial resistance to septoria tritici blotch in 238 European wheat varieties and breeding lines. Plant Pathology 55, 726–738.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ballantyne BJ (1983) Resistance to speckled leaf blotch of wheat in southern New South Wales. In ‘Septoria of Cereals: Proceedings Workshop’. (Ed. AL Scharen) pp. 31–32. (Montana State University: Bozeman, MT)

Bariana HS , Kaur K , Gill MB , Willey N (2004) Diversity of resistance to rust diseases in synthetic hexaploids. In ‘Proceedings of the 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders Assembly’. Canberra, ACT. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 13–15. (Royal Australian Chemical Institute: North Melbourne, Vic.)

Cheong J, Wallwork H, Williams KJ (2004) Identification of a major QTL for yellow leaf spot resistance in the wheat varieties Brookton and Cranbrook. Australian Journal of Agricultural Research 55, 315–319.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Eastwood RF, Lagudah ES, Appel R, Hannah M, Kollmorgen JE (1991) Triticum tauschii: a novel source of resistance to cereal cyst nematode (Heterodera avenae). Australian Journal of Agricultural Research 42, 69–77.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Eyal Z , Scharen AL , Prescott JM , van Ginkel M (1987) ‘The Septoria diseases of wheat: concepts and methods of disease management.’ (CIMMYT: Mexico)

Faris JD, Anderson JA, Francl LJ, Jordahl JG (1996) Chromosomal location of a gene conditioning insensitivity in wheat to a necrosis-inducing culture filtrate from Pyrenophora tritici-repentis. Phytopathology 86, 459–463.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Faris JD, Anderson JA, Francl LJ, Jordahl JG (1997) RFLP mapping of resistance to chlorosis induction by Pyrenophora tritici-repentis in wheat. Theoretical and Applied Genetics 94, 98–103.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Feldman M (2001) Origin of cultivated wheat. In ‘The world wheat book; a history of wheat breeding’. (Eds AP Bonjean, WJ Angus) (Lavoisier Publishing: Paris)

Fried PM (1989) Improved method to produce large quantities of Septoria nodorum inoculum. In ‘Septoria of cereals. Proceedings of the 3rd International Workshop on Septoria Diseases of Cereals’. (Ed. PM Fried) (Swiss Federal Research Station for Agronomy: Zurich-Reckenholz)

Hean KM, Lu H, Friesen TL, Faris JD (2004) Genomic targeting and high resolution mapping of the TSn1 gene in wheat. Crop Science 44, 951–962. open url image1

James WC (1971) An illustrated series of assessment keys for plant diseases, their preparation and usage. Canadian Plant Disease Survey 51, 39–65. open url image1

McIntosh RA , Wellings CR , Park RF (1995) ‘Wheat rusts: an atlas of resistance genes.’ p. 199. (CSIRO Publishing: Melbourne, Vic.)

McIntosh RA , Yamazaki Y , Devos KM , Dubcovsky J , Rogers WJ , Appels R (2003) Catalogue of gene symbols for wheat, Vol. 4. In ‘Proceedings of the 10th International Wheat Genetics Symposium’. Paestum, Italy. (S.I.M.I, Via N. Nisco 3/A-00179: Roma, Italy)

Mujeeb-Kazi A, Devila F, Villareal RL, Cortes A, Roases V, Delgado R (2001a) Registration of 10 synthetic hexaploid wheat and six bread wheat germplasm resistant to karnal bunt. Crop Science 41, 1652–1653. open url image1

Mujeeb-Kazi A, Cano S, Roases V, Cortes A, Delgado R (2001b) Registration of five synthetic hexaploid wheat and seven bread wheat germplasm resistant to wheat spot blotch. Crop Science 41, 1653–1654. open url image1

Ogbonnaya FC , Dreccer MF , Wilson J , Trethowan R , Borgognone G , Winter B , Eastwood RF , Milgate A , Shanker M , Mclean M , Hollaway G , Lagudah ES (2004) Spectrum of disease resistance in synthetic hexaploids. In ‘Proceedings of the 11th Wheat Breeding Assembly, ‘Seeding the Future’ Conference’. Canberra, 21–24 September 2004. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 155–158. (Royal Australian Chemical Institute: North Melbourne, Vic.)

Ogbonnaya FC , Halloran GM , Lagudah ES (2005) D genome of wheat—60 years on from Kihara, Sears and McFadden. In ‘Frontiers of wheat bioscience’. (Ed. Tsunewaki Koichiro) (Kihara Memorial Yokohama Foundation for the Advancement of Life Sciences: Yokohama, Japan)

Ogbonnaya FC, Seah S, Delibes A, Jahier J, López-Brana I, Eastwood RF, Lagudah ES (2001) Molecular-genetic characterisation of a new nematode resistance gene in wheat. Theoretical and Applied Genetics 102, 623–629.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Siedler H, Obst A, Hsam SLK, Zeller FJ (1994) Evaluation for resistance to Pyrenophora tritici-repentis in Aegilops tauschii Coss. and synthetic hexaploid amphiploids. Genetic Resources and Crop Evolution 41, 27–34.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Singh PK, Mergoum M, Ali S, Adhikari TB, Elias EM, Hughes GR (2006) Identification of new sources of resistance to tan spot, stagonospora nodorum blotch, and septoria tritici blotch of Wheat. Crop Science 46, 2047–2053.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Tadesse W, Hsam SLK, Zeller FJ (2006a) Evaluation of common wheat varieties for tan spot resistance and chromosomal location of resistance gene in the cultivar “Salamouni”. Plant Breeding 125, 318–322.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Tadesse W, Hsam SLK, Wenzel G, Zeller FJ (2006b) Identification and monosomic analysis of tan spot resistance genes in synthetic wheat lines (Triticum turgidum L, × Aegilops tauschii Coss.). Crop Science 46, 1212–1217.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Tekauz A (1985) A numerical scale to classify reactions of barley to Pyrenophora teres. Canadian Journal of Plant Pathology 7, 181–183. open url image1

Thompson JP, Haak MI (1997) Resistance to root-lesion nematode (Pratylenchus thornei) in Aegilops tauschii Coss., the D-genome donor to wheat. Australian Journal of Agricultural Research 48, 553–559.
| Crossref | GoogleScholarGoogle Scholar | open url image1

van Ginkel M, Ogbonnaya F (2007) Novel genetic diversity from synthetic wheats in breeding varieties for changing production conditions. Field Crops Research 104, 86–94.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Williams K, Taylor S, Bogacki P, Pallotta M, Bariana H, Wallwork H (2002) Mapping of the root lesion nematode (Pratylenchus neglectus) resistance gene Rlnn1 in wheat. Theoretical and Applied Genetics 104, 874–879.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Xu SS, Friesen TL, Mujeeb-Kazi A (2004) Seedling resistance to tap spot and Stagonospora nodorum blotch in synthetic hexaploid wheats. Crop Science 44, 2238–2245. open url image1

Yong-Bi F, Peterson GW, Richards KW, Sommers D, DePauw RM, Clarke JM (2005) Allelic reduction and genetic shift in the Canadian hard red spring wheat germplasm released from 1845 to 2004. Theoretical and Applied Genetics 100, 1505–1516.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Zwart RS, Thompson JP, Godwin ID (2005) Identification of quantitative trait loci for resistance to two species of root-lesion nematode (Pratylenchus thornei and P. neglectus) in wheat. Australian Journal of Agricultural Research 56, 345–352.
| Crossref | GoogleScholarGoogle Scholar | open url image1










Appendix 1.  Summary of disease ratings
Click to zoom