Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > AR07094
RESEARCH ARTICLE
Contents Vol 58(12)

The importance of the type and time of inoculation and assessment in the determination of resistance in Brassia napus and B. juncea to Sclerotinia sclerotiorum

C. X. Li A K , Hua Li B , A. B. Siddique A , K. Sivasithamparam B , P. Salisbury C , S. S. Banga D , Shashi Banga D , C. Chattopadhyay E , A. Kumar E , R. Singh F , D. Singh F , A. Agnihotri G , S. Y. Liu H , Y. C. Li H , J. Tu I , T. D. Fu I , Y. F. Wang J and M. J. Barbetti A
+ Author Affiliations
- Author Affiliations

A School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

B School of Earth and Geographical Sciences, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.

C School of Agriculture and Food Systems, The University of Melbourne, Victoria 3010, Australia.

D Department of Plant Breeding, Genetics & Biotechnology, Punjab Agricultural University, Ludhiana 141004, India.

E National Research Centre on Rapeseed-Mustard, Sewar, Bharatpur 321303, India.

F Oilseeds Section, C.C.S. Haryana Agricultural University, Hisar 125004, India.

G The Energy and Resources Institute, Lodhi Rd, New Delhi 110003, India.

H Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, P.R. China.

I The National Key Laboratory of Crop Genetics and Improvement, Huazhong Agricultural University, Wuhan 430070, P. R. China.

J Institute of Industrial Crops, Urumqi, P. R. China.

K Corresponding author. Email: caixiali@plants.uwa.edu.au

Australian Journal of Agricultural Research 58(12) 1198-1203 https://doi.org/10.1071/AR07094
Submitted: 25 June 2007  Accepted: 3 August 2007   Published: 17 December 2007

Abstract

Sclerotinia stem rot (SSR) is a significant agricultural problem worldwide. Finding sources of resistance is crucial to the ongoing search for better management of this disease. Brassica germplasm from Australia, China and India was screened for resistance to SSR under Western Australian field conditions following stem inoculation, application of a spray of mycelial suspension, or as a consequence of myceliogenic germination originating from sclerotia resident in soil. Significant differences in response were observed among 53 genotypes using each of the three screening methods. There was a variable impact of the time of inoculation on the disease level depending upon time of assessment post-stem inoculation. However, this impact could be reduced to an insignificant level provided the assessment after stem inoculation was delayed until 3 weeks post-inoculation. The results of these studies indicate that the use of appropriate inoculation and assessment methods could significantly reduce variability in the responses commonly observed in screening for resistance in crop plants against Sclerotinia sclerotiorum.


Acknowledgments

Our special thanks to the Australian Centre for International Agricultural Research and to the Grains Research and Development Corporation for funding this research.


References


Bailey DJ (1987) Screening for resistance to Sclerotinia sclerotiorum in oilseed rape using detached leaves. Tests of Agrochemicals and Cultivars 8, 152–153. open url image1

Boland GJ, Hall R (1994) Index of plant hosts of Sclerotinia sclerotiorum. Canadian Journal of Plant Pathology 16, 93–108. open url image1

Bradley CA, Henson RA, Porter PM, LeGare DG, del Rio LE, Khot SD (2006) Response of canola cultivars to Sclerotinia sclerotiorum in controlled and field environment. Plant Disease 90, 215–219.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Buchwaldt L , Li R , Hegedus DD , Rimmer SR (2005) Pathogenesis of Sclerotinia sclerotiorum in relation to screening for resistance. In ‘Proceedings of the 13th International Sclerotinia Workshop’. Monterey, CA. p. 22. (University of California Cooperative Extension: Salinas, CA)

Buchwaldt L , Yu FQ , Rimmer R , Hegedus DD (2003) Resistance to Sclerotinia sclerotiorum in a Chinese Brassica napus cultivar. In ‘Proceedings of the 8th International Congress of Plant Pathology’. Christchurch, New Zealand.

Hind-Lanoiselet TL , Lewington F (2004) ‘Canola concepts: managing sclerotinia.’ Agnote DPI-490. (New South Wales Agriculture)

Li YC , Chen J , Bennett R , Kiddle G , Wallsgrove R , Huang YJ , He YH (1999) Breeding, inheritance, and biochemical studies on Brassica napus cv. Zhougyou 821: tolerance to Sclerotinia sclerotiorum (stem rot). In ‘Proceedings of the 10th International Rapeseed Congress’. Canberra, Australia. (Eds N Wratten, PA Salisbury) p. 61.Available at: www.regional.org.au/au/gcirc/

Li CX, Li H, Sivasithamparam K, Fu TD, Li YC, Liu SY, Barbetti MJ (2006) Expression of field resistance under Western Australian conditions to Sclerotinia sclerotiorum in Chinese and Australian Brassica napus and Brassica juncea germplasm and its relation with stem diameter. Australian Journal of Agricultural Research 57, 1131–1135.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Coutu C, Hegedus DD (2004a) Interaction of Sclerotinia sclerotiorum with a resistant Brassica napus cultivar: expressed sequence tag analysis identifies genes associated with fungal pathogenesis. Fungal Genetics and Biology 41, 735–753.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Li R, Rimmer R, Buchwaldt L, Sharpe AG, Seguin-Swartz G, Hegedus DD (2004b) Interaction of Sclerotinia sclerotiorum with Brassica napus: cloning and characterization of endo- and exo-polygalacturonases expressed during saprophytic and parasitic modes. Fungal Genetics and Biology 41, 754–765.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Liu S, Wang D, Zhang J, Fitt BDL, Xu Z, Evans N, Liu Y, Yang W, Guo X (2005) In vitro mutation and selection of doubled-haploid Brassica napus lines with improved resistance to Sclerotinia sclerotiorum. Plant Cell Reports 24, 133–144.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Pratt RG, Dabney SM, Mays DA (1988) New forage legume hosts of Sclerotinia trifoliorum and S. sclerotiorum in the southeastern United States. Plant Disease 72, 593–596.
| Crossref |
open url image1

Purdy LH (1979) Sclerotinia sclerotiorum: history, diseases and symptomatology, host range, geographic distribution and impact. Phytopathology 69, 875–880. open url image1

Zhao J, Meng J (2003) Genetic analysis of loci associated with partial resistance to Sclerotinia sclerotiorum in rapeseed (Brassica napus L.). Theoretical and Applied Genetics 106, 759–764.
| PubMed |
open url image1

Zhao J, Peltier AJ, Meng J, Osborn TC, Grau CR (2004) Evaluation of Sclerotinia stem rot resistance in oilseed Brassica napus using a petiole inoculation technique under greenhouse conditions. Plant Disease 88, 1033–1039.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Zhao J, Udall J, Quijada P, Grau C, Meng J, Osborn T (2006) Quantitative trait loci for resistance to Sclerotinia sclerotiorum and its association with a homeologous non-reciprocal transposition in Brassica napus L. Theoretical and Applied Genetics 112, 509–516.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1