RAMP based fingerprinting and assessment of relationships among Australian narrow-leafed lupin (Lupinus angustifolius L.) cultivars
H. Yuan A , G. Yan B E , K. H. M. Siddique C and H. Yang C DA Department of Biotechnology, Zhanjiang Ocean University, Guangdong, Zhanjiang, 524088, China.
B School of Plant Biology, Faculty of Natural and Agricultural Sciences, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
C Centre for Legumes in Mediterranean Agriculture, The University of Western Australia, 35 Stirling Highway, Crawley, WA 6009, Australia.
D Department of Agriculture Western Australia, 3 Baron-Hay Court, South Perth, WA 6151, Australia.
E Corresponding author. Email: gyan@plants.uwa.edu.au
Australian Journal of Agricultural Research 56(12) 1339-1346 https://doi.org/10.1071/AR05188
Submitted: 30 May 2005 Accepted: 12 August 2005 Published: 15 December 2005
Abstract
Narrow-leafed lupin is a major winter grain legume crop in the Australian farming system and a number of commercial cultivars are currently available to growers. A significant level of polymorphism was detected in narrow-leafed lupin cultivars by the randomly amplified microsatellite polymorphism (RAMP) approach, suggesting that cultivars harbour considerable DNA variation. Seventy-seven cultivar-specific markers were found among the 23 lupin cultivars examined and a dichotomous fingerprinting key was developed for the molecular identification of lupin cultivars. Cluster analysis of pairwise distance matrix computed from RAMP profiles grouped the 23 cultivars into 4–5 clusters, which generally agreed with their pedigree relationships.
Additional keywords: RAMP, lupin cultivars, dichotomous molecular identification key.
Acknowledgments
H. Yuan acknowledges the financial support by CLIMA and the School of Plant Biology during her visit to Western Australia in 2004/2005.
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