AFLP and SSR analysis of genetic diversity among landraces of bread wheat (Triticum aestivum L. em. Thell) from different geographic regions
B. J. Stodart A , M. Mackay B and H. Raman A CA NSW Department of Primary Industries, Wagga Wagga Agricultural Institute, Wagga Wagga, NSW 2650, Australia.
B Australian Winter Cereals Collection, Tamworth Agricultural Institute, Tamworth, NSW 2340, Australia.
C Corresponding author. Email: harsh.raman@agric.nsw.gov.au
Australian Journal of Agricultural Research 56(7) 691-697 https://doi.org/10.1071/AR05015
Submitted: 18 January 2005 Accepted: 26 April 2005 Published: 22 July 2005
Abstract
A set of 44 bread wheat landraces was used to determine the efficacy of 16 amplifed fragment length polymorphism (AFLP) primers and 63 wheat simple sequence repeat (SSR) markers in identifying polymorphisms between accessions. The SSR markers detected approximately 10 alleles per locus with a mean gene diversity (Hz) of 0.63, whereas AFLP primers identified approximately 147 fragments per primer with a mean gene diversity of 0.25. A set of 54 SSR markers and 11 AFLP primers was identified as highly polymorphic (polymorphic information content (PIC) ≥ 0.5 and 0.3 for SSR and AFLP, respectively), and suitable for molecular characterisation of germplasm. Principle coordinate analysis suggested that the AFLP and SSR loci could be used to discriminate among accessions collected from North Africa and southern Europe from those collected from the Middle East. Both marker types indicate that accessions from North Africa and southern Europe, the Middle East, and southern and eastern Asia are genetically diverse. The results indicate the usefulness of the molecular markers to assess genetic diversity present within germplasm collections.
Additional keywords: molecular diversity, principal coordinates analysis.
Acknowledgments
The authors acknowledge the financial support given under the Preservation of Biological Assets program by the BioFirst initiative of the New South Wales Government, Australia.
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