Register      Login
Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
Shopping Cart: ( empty )
You are here: Home > Journals > CP > AR04085
RESEARCH ARTICLE
Contents Vol 55(12)

Identification of microsatellite markers associated with the cereal cyst nematode resistance gene Cre3 in wheat

E. M. Martin A C D , R. F. Eastwood B C and F. C. Ogbonnaya A C
+ Author Affiliations
- Author Affiliations

A Department of Primary Industries (Primary Industries Research Victoria), Private Bag 260, Horsham, Vic. 3401, Australia.

B Australian Grain Technologies, Private Bag 260, Horsham, Vic. 3401, Australia.

C Molecular Plant Breeding Cooperative Research Centre, Suite 21, 2 Park Drive, Bundoora, Vic. 3083, Australia.

D Corresponding author. Email: erica.martin@dpi.vic.gov.au

Australian Journal of Agricultural Research 55(12) 1205-1211 https://doi.org/10.1071/AR04085
Submitted: 13 April 2004  Accepted: 22 October 2004   Published: 21 December 2004

Abstract

Cereal cyst nematode (CCN) is a root disease caused by the pathogen Heterodera avenae Woll. that significantly reduces wheat (Triticum aestivum L.) grain yields in temperate countries. The Cre3 gene, located on chromosome 2DL, provides high levels of resistance to the Australian pathotype and isolates from Syria and Algeria, and has become available to wheat breeders. Selection for lines carrying the Cre3 gene in Australian wheat breeding programs is currently based on a dominant PCR marker (Cre3spf/r) diagnostic for the Cre3 gene. However, this marker has limitations that increase the cost and reduce selection efficiency in screening early-generation breeding lines. Such limitations would be minimised by the identification of a microsatellite marker linked to the Cre3 gene. We have constructed 2 genetic linkage maps of wheat chromosome 2DL and identified microsatellite markers mapping closely to the diagnostic Cre3spf/r marker. These closely linked markers were validated in a diverse range of germplasm, and one microsatellite marker, Xgwm301, which mapped 4 cM from Cre3spf/r, was shown to be highly associated with the presence of the Cre3 gene. Amplification conditions for the Xgwm301 locus were optimised, and its use in marker-assisted selection to identify Cre3 CCN-resistant wheat in the Australian Grain Technologies breeding program is demonstrated.

Additional keywords: Aegilops tauschii, MAS, molecular marker, pyramiding, SSR.


Acknowledgments

The authors would like to acknowledge the financial support of the Grains Research and Development Cooperation and the support of the CRC for Molecular Plant Breeding. They would like to thank Dr Howard Eagles for his valuable comments on the manuscript, Dr Livinus Emebiri for his assistance with the mapping work and Jayne Wilson for her contributions with the biological assay.


References


Blair MW, McCouch SR (1997) Microsatellite and sequence-tagged site markers diagnostic for the rice bacterial leaf blight resistance gene xa-5. Theoretical and Applied Genetics 95, 174–184.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Bougot Y, Lemoine J, Pavoine MT, Barloy D, Doussinault G (2002) Identification of a microsatellite marker associated with Pm3 resistance alleles to powdery mildew in wheat. Plant Breeding 121, 325–329.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Brennan JP, Murray GM (1988) Australian wheat diseases: assessing their economic importance. Agricultural Science 1, 26–35. open url image1

Cakir M, Galway N, Ablett G, Poulsen D, Appels R, Wellings C, Vivar H (2002) Mapping QTLs for resistance to stripe rust in barley (Hordeum vulgare L.). ‘Plant breeding for the millennium. Proceedings of the 12th Australasian Plant Breeding Conference’. Perth, W. Aust. (Ed. JA McComb ) pp. 376–378. (Australasian Plant Breeding Association Inc.)


Chalmers KJ, Campbell AW, Kretschmer J, Karakousis A, Henschke PH , et al. (2001) Construction of three linkage maps in bread wheat, Triticum aestivum. Australian Journal of Agricultural Research 52, 1089–1119.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Cordeiro GM, Christopher MJ, Henry RJ, Reinke RF (2002) Identification of microsatellite markers for fragrance in rice by analysis of the rice genome sequence. Molecular Breeding 9, 245–250.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Eagles HA, Bariana HS, Ogbonnaya FC, Rebetzke GJ, Hollamby GJ, Henry RJ, Henschke PH, Carter M (2001) Implementation of markers in Australian wheat breeding. Australian Journal of Agricultural Research 52, 1349–1356.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Eastwood RF (1995) Genetics of resistance to Heterodera avenae in Triticum tauschii and its transfer to bread wheat. PhD thesis, University of Melbourne.

Eastwood RF, Lagudah ES, Appels R (1994) A directed search for DNA sequences tightly linked to cereal cyst nematode resistance genes in Triticum tauschii. Genome 37, 311–319. open url image1

Eastwood RF, Lagudah ES, Apples R, Hannah M, Kollmorgen JF (1991) Triticum tauschii: a novel source of resistance to cereal cyst nematode (Heterodera avenae). Australian Journal of Agricultural Research 42, 69–77. open url image1

Gororo NN, Eagles HA, Eastwood RF, Nicolas ME, Flood RG (2002) Use of Triticum tauschii to improve yield of wheat in low-yielding environments. Euphytica 123, 241–254.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Gupta PK, Balyan HS, Edwards KJ, Isaac P, Korzun V , et al. (2002) Genetic mapping of 66 new microsatellite (SSR) loci in bread wheat. Theoretical and Applied Genetics 105, 413–422.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Harker N, Rampling LR, Shariflou MR, Hayden MJ, Holton TA, Morell MK, Sharp PJ, Henry RJ, Edwards KJ (2001) Microsatellites as markers for Australian wheat improvement. Australian Journal of Agricultural Research 52, 1121–1130.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Huang XQ, Coster H, Ganal MW, Roder MS (2003a) Advanced backcross QTL analysis for the identification of quantitative trait loci alleles from wild relatives of wheat (Triticum aestivum L.). Theoretical and Applied Genetics 106, 1379–1389.
| PubMed |
open url image1

Huang XQ, Wang LX, Xu MX, Roder MS (2003b) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theoretical and Applied Genetics 106, 858–865.
| PubMed |
open url image1

Karakousis A, Chalmers K, Barr A, Langridge P (2000) Identification of SSR markers for use in the Australian barley breeding program. ‘Proceeding from the 8th International Barley Genetics Symposium’. Adelaide, S. Aust..


Korzun V, Roder MS, Ganal MW, Worland AJ, Law CN (1998) Genetic analysis of the dwarfing gene (Rht8) in wheat. Part I. Molecular mapping of Rht8 on the short arm of chromosome 2D of bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 96, 1104–1109.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Lagudah ES, Moullet O, Appels R (1997) Map-based cloning of a gene sequence encoding a nucleotide-binding domain and a leucine-rich region at the Cre3 nematode resistance locus of wheat. Genome 40, 659–665.
| PubMed |
open url image1

Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newburg L (1987) MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1, 174–181.
| Crossref | PubMed |
open url image1

Langridge P, Lagudah ES, Holton TA, Appels R, Sharp PJ, Chalmers KJ (2001) Trends in genetic and genome analyses in wheat: a review. Australian Journal of Agricultural Research 52, 1043–1077.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Liu XM, Smith CM, Gill BS, Tolmay V (2001) Microsatellite markers linked to six Russian wheat aphid resistance genes in wheat. Theoretical and Applied Genetics 102, 504–510.
| Crossref | GoogleScholarGoogle Scholar | open url image1

de Majnik J, Ogbonnaya FC, Moullet O, Lagudah ES (2003) The Cre1 and Cre3 nematode resistance genes are located at homeologous loci in the wheat genome. Molecular Plant-Microbe Interactions 16, 1129–1134.
| PubMed |
open url image1

Ogbonnaya FC, Seah S, Delibes A, Jahier J, Lopez-Brana I, Eastwood RF, Lagudah ES (2001a) Molecular-genetic characterisation of a new nematode resistance gene in wheat. Theoretical and Applied Genetics 102, 623–629.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Ogbonnaya FC, Subrahmanyam NC, Moullet O, de Majnik J, Eagles HA, Brown JS, Eastwood RF, Kollmorgen J, Appels R, Lagudah ES (2001b) Diagnostic DNA markers for cereal cyst nematode resistance in bread wheat. Australian Journal of Agricultural Research 52, 1367–1374.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Paull JG, Chalmers KJ, Karakousis A, Kretschmer JM, Manning S, Langridge P (1998) Genetic diversity in Australian wheat varieties and breeding material based on RFLP data. Theoretical and Applied Genetics 96, 435–466.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Pestsova E, Ganal MW, Roder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43, 689–697.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Prasad M, Varshney RK, Kumar A, Balyan HS, Sharma PC, Edwards KJ, Singh H, Dhaliwal HS, Roy JK, Gupta PK (1999) A microsatellite marker associated with a QTL for grain protein content on chromosome arm 2DL of bread wheat. Theoretical and Applied Genetics , 341–345. open url image1

Raman H, Moroni JS, Sato K, Read BJ, Scott BJ (2002) Identification of AFLP and microsatellite markers linked with an aluminium tolerance gene in barley (Hordeum vulgare L.). Theoretical and Applied Genetics 105, 458–464.
| Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Rivoal R, Bekal S, Valette S, Gauthier J-P, Bel Hadj Fradj M, Mokabli A, Jahier J, Nicol J, Yahyaoui A (2001) Variation in reproductive capacity and virulence on different genotypes and resistance genes of Triticeae, in the cereal cyst nematode species complex. Nematology 3, 581–592.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier M-H, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149, 2007–2023.
| PubMed |
open url image1

Romero MD, Montes MJ, Sin E, Lopez-Brana I, Duce A, Martin-Sanchez JA, Andres MF, Delibes A (1998) A cereal cyst nematode (Heterodera avenae Woll.) resistance gene transferred from Aegilops triuncialis to hexaploid wheat. Theoretical and Applied Genetics 96, 1135–1140.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Slootmaker LAJ, Lange W, Jochemsen G, Schepers J (1974) Monosomic analysis in bread wheat of resistance to cereal root eelworm. Euphytica 23, 497–503.
| Crossref |
open url image1

Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad-spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Science 43, 333–336. open url image1

Warner P, Karakousis A, Schiemann A, Eglinton J, Langridge P, Barr A (2001) An investigation of rapid DNA extraction methods for routine MAS in the SA barley improvement program. ‘Proceedings of the 10th Barley Technical Symposium’. Canberra, ACT. (2001 Australian Barley Technical Symposium Inc.)


Williams KJ, Fisher JM, Langridge P (1994) Identification of RFLP markers linked to the cereal cyst nematode resistance gene (Cre) in wheat. Theoretical and Applied Genetics 89, 927–930.
| Crossref | GoogleScholarGoogle Scholar | open url image1

Xu Y (2004) Developing marker-assisted selection strategies for breeding hybrid rice. ‘Plant breeding reviews Vol 23’. (Ed. J Janick) pp. 73–174. (John Wiley and Sons, Inc: Hoboken, NJ)