Segregation of glutenins in wheat × maize-derived doubled haploid wheat populations

Abstract

The segregation of both high and low molecular weight glutenin subunits across 7 F₁ wheat (Triticum aestivum L.) × maize (Zea mays L.) derived doubled haploid populations was examined. The F₁ wheats used in each population were produced from parents of interest to Australian wheat breeding programs. The parents varied by up to 5 glutenin subunit loci. Examination of subunits individually within each population using a chi-square analysis revealed that all but 2 of the 26 pairs of alleles analysed fitted the expected 1 : 1 segregation ratio. Glutenin profiles were examined for each cross individually and all but one (Sonalika/Hartog) fitted the expected Mendelian segregation pattern. The analysis of allele distribution of the 6 glutenin loci across all 7 crosses showed all falling well within expected segregation ratios. Closer examination of parental lines and populations revealed irregularities which conflict with original assumptions and provide a valid explanation for the few segregation distortions observed. It is concluded that wheat × maize-derived doubled haploid populations represent a unbiased assortment of parental gametes on both arms of Group 1 chromosomes.